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Journals / Selçuk Üniversitesi Fen Fakültesi Fen Dergisi / 2010 / Cilt: 2 - Sayı: 35

Determination of Genetic Diversity among Phaseolus vulgaris (Leguminosae) Populations Collected from Konya Region by SDS-PAGE

Konya Bölgesinden Toplanan Phaseolus vulgaris (Leguminosae) Populasyonları Arasındaki Genetik Çeşitliliğin SDS-PAGE Yöntemi ile Belirlenmesi

Elif GÜLBAHÇE
Buğra ÖZKUBAT
Emine ARSLAN
Pages
59–64
Publication Date
2010-12-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

While the highest similarity was found between Kanada (Çumra) and Sarnıç (Altınekin) genotypes, the most distant genotype was Sarhoş (Washington) (Derbent).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study aimed to determine the genetic diversity among 17 Phaseolus vulgaris populations collected from districts of Konya by SDS-PAGE.
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Key Findings & Data:Seed storage protein profiles were scored visually as present (1) or absent (0).
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Conclusion & Impact:While the highest similarity was found between Kanada (Çumra) and Sarnıç (Altınekin) genotypes, the most distant genotype was Sarhoş (Washington) (Derbent).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Phaseolus vulgaris · Genetik çeşitlilik · SDS-PAGE

Abstract

This study aimed to determine the genetic diversity among 17 Phaseolus vulgaris populations collected from districts of Konya by SDS-PAGE. Seed storage protein profiles were scored visually as present (1) or absent (0). In all, 37 polypeptide bands of different sizes ranging from 14.4 to ~135 kDa were observed in the 17 Phaseolus vulgaris populations. Cluster analysis was performed using the UPGMA in Bio 1 D ++ computer program. Similarity among genotypes was estimated basing on Nei’s homology and a dendrogram was obtained. According to dendrogram, 17 populations belonging to Phaseolus vulgaris were similar in rates ranging from 68 to 100 %. While the highest similarity was found between Kanada (Çumra) and Sarnıç (Altınekin) genotypes, the most distant genotype was Sarhoş (Washington) (Derbent).

Özet

Bu çalışmada Konya’nın ilçelerinden toplanan 17 Phaseolus vulgaris populasyonu arasındaki genetik çeşitliliğin SDS-PAGE ile belirlenmesi amaçlanmıştır. Tohum depo protein profilleri görsel şekilde var (1) ya da yok (0) olarak skorlanmıştır. On yedi Phaseolus vulgaris populasyonunda, 14.4 ve ~135 kDa arasında değişen farklı büyüklüklerde toplamda 37 polipeptit bant gözlenmiştir. Bio1D++ bilgisayar programında UPGMA kullanılarak kümeleme analizi yapılmıştır. Genotipler arasındaki benzerlik Nei’nin homolojisine dayanarak tahmin edilmiş ve bir dendrogram elde edilmiştir. Dendrograma göre Phaseolus vulgaris’e ait 17 populasyon %68-100 arasında değişen oranlarda benzer bulunmuştur. En yüksek benzerlik Kanada (Çumra) ve Sarnıç (Altınekin) genotipleri arasında bulunurken, en uzak genotip Sarhoş (Washington) (Derbent) olmuştur.

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Cite This Article

GÜLBAHÇE, E., ÖZKUBAT, B., ARSLAN, E. (2010). Determination of Genetic Diversity among Phaseolus vulgaris (Leguminosae) Populations Collected from Konya Region by SDS-PAGE. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, Cilt: 2 - Sayı: 35, 59–64. https://ajindex.com/articles/800421

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References (10)

  1. Babaoğlu, S., Açık, L., Çelebi, A., Adıgüzel, N. Molecular Analysıs Of Turkısh Alyssum L. (Brassıcaceae) Specıes By Rapd-Pcr And Sds-Page Methods. G.U. Journal of Science 17(3):25-33, (2004).
  2. Beyazbenli, Ş., Dural, H., Arslan, E., Ertuğrul, K. Konya Bölgesindeki bazı Vicia L. (Leguminosae) Türlerinin Tohum Protein Profillerinin SDS-PAGE Yöntemiyle İle Belirlenmesi. S.Ü. Fen Edebiyat Fak. Fen Dergisi, 28: 117-122, ( 2006).
  3. Chen L.F.O., Cheng M.C., Chen S.C.G. Similarity and diveristy of seed proteins in rice varieties. Botanical Bulletin of Academia Sinica, 28: 169–183, (1987).
  4. Fujiwara, T., Nambarab E., Yamagishib K., Gotoc D. B., Naitoc, S. Storage Proteins. American Society of Plant Biologists, 1-12, (2002).
  5. Ghafoor, A, Ahmad, Z, Qureshi, A.S, Bashir, M. Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE. Euphytica, 123:367-378, (2000).
  6. Ladizinsky G.W., Hymowitz T. Seed protein electrophoresis in taxonomic and evolutionary studies. Theoretical and Applied Genetics, 54: 680–685, (1979).
  7. Sözen, Ö. Artvin ili yerel fasulye (Phaseolus vulgaris L.) populasyonlarının toplanması, tanımlanması ve morfolojik varyabilitesinin belirlenmesi. Yüksek lisans tezi, Ondokuz Mayıs Üniversitesi,
  8. Fen Bilimleri Enstitüsü, Samsun, sayfa 1, (2006).
  9. Liang, X.Q., Luo, M., Holbrook, C.C., Guo, B.Z. Storage protein profiles in Spanish and runner market type peanuts and potential markers. BMC Plant Biology, 6:24, (2006).
  10. Singh S.P., Nodari R., Gepts P. Genetic diversity in cultivated common bean I. allozymes. Crop Science, 31:19-23, (1991). 0] Yupsanis T.M., Moustakes M., Karakoli S. Seed protein electrophoresis for varietal identification in rice (Oryza sativa L.), Journal of Agronomy and Crop Science, 168:95–99, (1992). 1] Przybylska J., Zimniak-Przybylska Z., Krajewski P. Diversity of seed albumins in the grass pea (Lathyrus sativus): an electrophoretic study, Genetic Resources and Crop Evolution, 45: 423–431, (1998). 2] Ertugrul, K., Arslan, E., Tugay, O. Characterization of Consolida S.F. Gray (Ranunculaceae) taxa in Turkey by seed storage protein electrophoresis. Turkish Journal of Biochemistry, 35(2);97-102, (2010). 3] Tamkoc, A., Arslan, E. Comparison of Agronomic Characters, Total Seed Storage Proteins and Their Use For Genotypes Discrimination in the Kentucky Bluegrass (Poa pratensis L.), Biotechnology and Biotechnological Equipment, 24(1):1573-1576, (2010). 4] Parisi, E., Agulari D., Methods Differen