Journals / Journal of Anatolian Environmental and Animal Sciences / 2016 / Cilt: 1 - Sayı: 1

Vibrio Infection and Treatment on the Juvenile Rainbow Trout (Oncorhynchus mykiss) Transferred Seawater

Pages
13–20
Publication Date
2016-10-30
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Results of the testing susceptibility to antibiotics showed that V. anguillarum isolates were resistant to sulphamethoxazole, ampicillin, erythromycin andoxytetracyclin, but all strains were found susceptible to oxolinic acid, enrofloxacin and florfenicol.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study is to determinethe antibiotic profile, serotyping and identification of isolated bacteria from disease outbreaks in cultured rainbow trout juveniles transferred to seawater at 8°Cfloating net cages in the Eastern Black Sea Region of Turkey.
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Key Findings & Data:The kidney and spleen of diseased rainbow trout that showed typical disease symptoms was streakedon the surface of T-TSA (added with 1.5% sodium chloride) and TCBS, and was incubated in the cooled incubator at 20±1°C for 48 hours.
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Conclusion & Impact:Results of the testing susceptibility to antibiotics showed that V. anguillarum isolates were resistant to sulphamethoxazole, ampicillin, erythromycin andoxytetracyclin, but all strains were found susceptible to oxolinic acid, enrofloxacin and florfenicol.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Rainbow trout · V. anguillarum · API 20E

Abstract

The aim of this study is to determinethe antibiotic profile, serotyping and identification of isolated bacteria from disease outbreaks in cultured rainbow trout juveniles transferred to seawater at 8°Cfloating net cages in the Eastern Black Sea Region of Turkey. The kidney and spleen of diseased rainbow trout that showed typical disease symptoms was streakedon the surface of T-TSA (added with 1.5% sodium chloride) and TCBS, and was incubated in the cooled incubator at 20±1°C for 48 hours. To identify of isolatedbacteria was carried out by using slide agglutination, API 20E, PCR and conventional biochemical tests. V. anguillarum isolates were detected serotype O1, andsequenced PCR products were confirmed to be similar to V. anguillarum strain at the rate of 99% (with accession numbers: LK021130) compered in GenBankdatabase. Results of the testing susceptibility to antibiotics showed that V. anguillarum isolates were resistant to sulphamethoxazole, ampicillin, erythromycin andoxytetracyclin, but all strains were found susceptible to oxolinic acid, enrofloxacin and florfenicol.

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Cite This Article

BALTA, F., BALTA, Z. D. (2016). Vibrio Infection and Treatment on the Juvenile Rainbow Trout (Oncorhynchus mykiss) Transferred Seawater. Journal of Anatolian Environmental and Animal Sciences, Cilt: 1 - Sayı: 1, 13–20. https://ajindex.com/articles/773878

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References (2)

  1. Akaylı T., Ürkü Ç. ve Özgür Ç., (2013). Kültür gökkuşağı alabalıklar (Oncorhynchus mykiss, Walbaum 1792)’ından izole edilen Gram-negatif bakterilerin antibiyotik duyarlılığı. Biyoloji Bilimleri Araştırma Dergisi, 6, 11-15.
  2. Akinbowale OL., Peng H. and Barton MD., (2006). Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. J. Appl. Microbiol., 100, 1103-1113.