SavedAdd your journal

Journals / International Journal of Agriculture Environment and Food Sciences / 2022 / Cilt: 6 - Sayı: 2

Profiling of non-pathogenic bacterial population by MALDI-TOF mass spectrometry in stone fruits

Serkan BAYMAN
Hamit KAVAK
Pages
220–225
Publication Date
2022-06-15
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was concluded that the most concentrated bacteria in the stone fruits was Stenotrophomonas rhizophila with 13 isolates, followed by respectively Bacillus megaterium with 9 isolates, Pantoea agglomerans with 7 isolates, Bacillus pumilus with 6 isolates, Xanthomonas hortorum with 5 isolates, Bacillus mojavensis and Rahnella aquatilis with 3 isolates

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The study was carried out to investigate the status of non-pathogenic bacteria isolated from infected plant tissues in stone fruit orchards including almonds, apricots, cherries, mahaleb, olives and plums in Adıyaman, Diyarbakır and Mardin provinces of Turkey.
📊
Key Findings & Data:Surveys were performed in the mentioned provinces between March and August in 2019-2021.
💡
Conclusion & Impact:It was concluded that the most concentrated bacteria in the stone fruits was Stenotrophomonas rhizophila with 13 isolates, followed by respectively Bacillus megaterium with 9 isolates, Pantoea agglomerans with 7 isolates, Bacillus pumilus with 6 isolates, Xanthomonas hortorum with 5 isolates, Bacillus mojavensis and Rahnella aquatilis with 3 isolates
🔬Design:Survey & Empirical Study
📍Scope:2019-2021 · 87 samples
⚙️Variables:Drupe · MALDI-TOF · Non-pathogenic bacteria

Abstract

The study was carried out to investigate the status of non-pathogenic bacteria isolated from infected plant tissues in stone fruit orchards including almonds, apricots, cherries, mahaleb, olives and plums in Adıyaman, Diyarbakır and Mardin provinces of Turkey. Surveys were performed in the mentioned provinces between March and August in 2019-2021. Survey studies showed that, 87 samples with typical bacterial disease symptoms were collected from 34 different stone fruit orchards. Hypersensitivity (HR) and host pathogenicity tests were performed following isolation from diseased plant tissues in the samples. A total of 70 isolates, which were found to be non-pathogenic with negative HR and host pathogenicity tests, were definitively diagnosed by MALDI-TOF analysis method. Finally, it was specified that bacteria of Bacillus and Pseudomonas genera were more densely colonized in different tissues of stone fruits. It was concluded that the most concentrated bacteria in the stone fruits was Stenotrophomonas rhizophila with 13 isolates, followed by respectively Bacillus megaterium with 9 isolates, Pantoea agglomerans with 7 isolates, Bacillus pumilus with 6 isolates, Xanthomonas hortorum with 5 isolates, Bacillus mojavensis and Rahnella aquatilis with 3 isolates

🔖

Cite This Article

BAYMAN, S., KAVAK, H. (2022). Profiling of non-pathogenic bacterial population by MALDI-TOF mass spectrometry in stone fruits. International Journal of Agriculture Environment and Food Sciences, Cilt: 6 - Sayı: 2, 220–225. https://ajindex.com/articles/765140

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (8)

  1. Ahmad, F., Siddiqui, M. A., Babalola, O. O., Wu, H. F. (2012). Biofunctionalization of nanoparticle assisted mass spectrometry as biosensors for rapid detection of plant associated bacteria. Biosensors and Bioelectronics, 35(1), 235-242.
  2. Aktan, Z. C. C., Soylu, S. (2020). Diyarbakır ilinde yetişen badem ağaçlarından endofit ve epifit bakteri türlerinin izolasyonu ve bitki gelişimini teşvik eden mekanizmalarının karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(3), 641-654. (in Turkish) Alavi, P., Starcher, M., Zachow, C., Müller, H., Berg, G. (2013). Root-microbe systems: the effect and mode of interaction of stress protecting agent (SPA) Stenotrophomonas rhizophila DSM14405T. Frontiers in Plant Science, 4, 141. Barash, I., Manulis-Sasson, S. (2007). Virulence mechanisms and host specificity of gall-forming Pantoea agglomerans. TRENDS in Microbiology, 15(12), 538-545.
  3. Berg, G., Knaape, C., Ballin, G., Seidel, D. (1994). Biological control of Verticillium dahliae Kleb. by natural occurring rhizosphere bacteria. Archives of Phytopathology and Plant Protection, 29(3), 249-262.
  4. Bremer, H. (1954). Türkiye Fitopatolojisi. Zirai Vekalet ve Haberleşme Müdürlüğü. Ankara. S. 295. (in Turkish) Bülbül, M., Mirik, M. (2014). Prevalence, isolation and identification of bacterial cancer pathogens on sweet cherry trees in Tekirdağ. The Journal of Turkish Phytopathology, 43: (1-2-3) 15-24.
  5. Costa, J., Pothier, J. F., Boch, J., Stefani, E., Jacques, M. A., Catara, V., Koebnik, R. (2021). Integrating science on Xanthomonadaceae for sustainable plant disease management in Europe. Molecular Plant Pathology, 22(12), 1461. Dunne, C., Moënne‐Loccoz, Y., McCarthy, J., Higgins, P., Powell, J., Dowling, D. N., O'gara, F. (1998). Combining proteolytic and phloroglucinol‐producing bacteria for improved biocontrol of Pythium‐mediated damping‐off of sugar beet. Plant pathology, 47(3), 299-307.
  6. Ernst, M., Silva, D. B., Silva, R., Monge, M., Semir, J., Vencio, R. Z., Lopes, N. P. (2015). A metabolomic protocol for plant systematics by matrix-assisted laser-desorption/ionization time-of flight mass spectrometry. Analytica chimica acta, 859, 46-58. FAO, (2020). Crops and livestock products. Erişim: [https://www.fao.org/faostat/en/#data/QCL]. Erişim Tarihi: 17.01.2022
  7. Gnanamanickam, S. S., Immanuel, J. E. (2007). Epiphytic bacteria, their ecology and functions. Plant-associated bacteria, 131-153.
  8. Gormez, A., Sahin, F. (2012). Determination of the pathogenic and