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Journals / Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi / 2019 / Cilt: 3 - Sayı: 2

Investigation of Cytotoxic Effect of Monoterpenes Beta-Citronellol and (-)-Menthone in Human Breast Cancer (MCF-7) Cell Line

Beta-Sitronellol ve (-)-Menton Monoterpenlerinin İnsan Meme Kanseri (MCF-7) Hücre Hattında Sitotoksik Etkilerinin İncelenmesi

Sevcan MAMUR
Pages
111–119
Publication Date
2019-05-27
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further studies should be conducted to determination cytotoxicity using different cell lines.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Monoterpenes are the primary components of plant essential oils which can use in medicines, food or cosmetic industries.
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Key Findings & Data:However no enough data are found about cytotoxicity of these compounds on human breast cancer (MCF-7) cells.
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Conclusion & Impact:Further studies should be conducted to determination cytotoxicity using different cell lines.
🔬Design:Research Article
📍Scope:2000 Verileri
⚙️Variables:Beta-Sitronellol · (-)-Menton · MCF-7

Abstract

Objective: Monoterpenes are the primary components of plant essential oils which can use in medicines, food or cosmetic industries. Many terpenoids exhibit a various pharmacological activities such as antiallergic, antiviral, anti-inflammatory and anticancer. However no enough data are found about cytotoxicity of these compounds on human breast cancer (MCF-7) cells. The aim of this study was to evaluate the potential cytotoxic effects of monoterpenes Beta-Citronellol (BC) and (-)-Menthone (MNT) using 3-(4,5-dimethylthiazolyl-2)-2,5 diphenyl tetrazolium bromide (MTT) assay in MCF-7 cell line.Methods: In this study, the cytotoxic effects of monoterpenes BC and MNT were analyzed by MTT assay at 24 and 48 hour treatment times in the MCF-7 cell line. MCF-7 cells were treated with different concentrations of BC (2, 10, 50, 250, 1250 μg/mL) and MNT (16, 80, 400, 2000, 10.000 μg/mL). Results: BC and MNT were significantly decreased cell viability (%) at all concentrations at 24-h treatment period. Furthermore, BC (250, 1250 μg/mL) and MNT (16, 400, 2000, 10.000 μg/mL) were significantly reduced the cell viability at 48-h treatment. Conclusion: As a result, BC and MNT showed cytotoxic effect on MCF-7 cells in both treatment times. Further studies should be conducted to determination cytotoxicity using different cell lines.

Özet

Amaç: Monoterpenler, ilaç, gıda veya kozmetik endüstrisinde kullanılan, bitki esans yağlarının ana bileşenleridir. Birçok terpenoid, antialerjik, antiviral, anti-inflamatuar ve antikanser gibi çeşitli farmakolojik aktiviteler sergiler. Bununla birlikte, bu bileşiklerin insan meme kanseri (MCF-7) hücreleri üzerine sitotoksik etkileri ile ilgili yeterli çalışma bulunmamaktadır. Bu çalışmanın amacı, Beta-Sitronellol (BS) and (-)-Menton (MNT) monoterpenlerinin potansiyel sitotoksik etkilerini MCF-7 hücre hattında 3-(4,5-dimethylthiazolyl-2)-2,5 diphenyl tetrazolyum bromid (MTT) testi ile incelemektir. Yöntem: Bu çalışmada BS ve MNT monoterpenlerinin sitotoksik etkileri, MCF-7 hücre hattında 24 ve 48 saat muamele sürelerinde MTT testi ile analiz edilmiştir. Bunun için hücrelere BS (2, 10, 50, 250, 1250 μg/mL) ve MNT (16, 80, 400, 2000, 10.000 μg/mL)’nin farklı konsantrasyonları uygulanmıştır.Bulgular: BS ve MNT, 24 saatlik uygulama süresinde tüm konsantrasyonlarda, (%) hücre canlılığını önemli düzeyde azaltmıştır. Ayrıca 48 saatlik uygulamada, BS (250, 1250 μg/mL) ve MNT (16, 400, 2000, 10.000 μg/mL), (%) hücre canlılığını anlamlı oranda düşürmüştür. Sonuç: Sonuç olarak; BS ve MNT, her iki muamele süresinde de MCF-7 hücreleri üzerinde sitotoksik etki göstermiştir. Ancak sitotoksisitenin belirlenmesine yönelik farklı hücre hatlarında daha fazla çalışma yapılmalıdır.

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Cite This Article

MAMUR, S. (2019). Investigation of Cytotoxic Effect of Monoterpenes Beta-Citronellol and (-)-Menthone in Human Breast Cancer (MCF-7) Cell Line. Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi, Cilt: 3 - Sayı: 2, 111–119. https://ajindex.com/articles/728490

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