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Journals / Turkish Journal of Biology / 2018 / Cilt: 42 - Sayı: 2

Recombinant outer membrane protein Q and putative lipoprotein from <i>Bordetella pertussis</i> inducing strong humoral response were not protective alone in the murine lung colonization model

Çiğdem YILMAZ
Erkan ÖZCENGİZ
Gülay ÖZCENGİZ
Pages
123–131
Publication Date
2018-04-27
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

However, there were no significant differences between test and control groups regarding interferon-gamma (IFN-γ) levels, and the mice lung colonization experiments indicated that neither rOmpQ nor rLpp could confer protection alone against B . pertussis challenge.

📌 3 Key Takeaways

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Objective & Hypothesis:Despite high vaccination coverage after introduction of whole cell (wP) and acellular pertussis (aP) vaccines, pertussis resurgence has been reported in many countries. aP vaccines are commonly preferred due to side effects of wP vaccines and formulated with aluminum hydroxide (Alum), which is not an effective adjuvant to eliminate Bordetella pertussis.
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Key Findings & Data:Both recombinant OmpQ and putative Lpp induced a significant increase in immunoglobulin G1 (IgG1) and immunoglobulin G2a (IgG2a) responses compared to the control group.
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Conclusion & Impact:However, there were no significant differences between test and control groups regarding interferon-gamma (IFN-γ) levels, and the mice lung colonization experiments indicated that neither rOmpQ nor rLpp could confer protection alone against B . pertussis challenge.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Adjuvants · Bordetella pertussis · pertussis vaccines

Abstract

Despite high vaccination coverage after introduction of whole cell (wP) and acellular pertussis (aP) vaccines, pertussis resurgence has been reported in many countries. aP vaccines are commonly preferred due to side effects of wP vaccines and formulated with aluminum hydroxide (Alum), which is not an effective adjuvant to eliminate Bordetella pertussis. Low efficiency of current aP vaccines is thought to be the main reason for the resurgence for which newer generation aP vaccines are needed. In the present study, immunogenicity and protective efficacy of outer membrane protein Q (OmpQ) and a putative lipoprotein (Lpp) from B. pertussis were investigated in mice by using two different adjuvants, monophosphoryl lipid A (MPLA) or Alum. OmpQ and putative Lpp were cloned, expressed, and purified from Escherichia coli. The proteins were formulated to immunize mice. Both recombinant OmpQ and putative Lpp induced a significant increase in immunoglobulin G1 (IgG1) and immunoglobulin G2a (IgG2a) responses compared to the control group. Moreover, MPLA-adjuvanted formulations resulted in higher IgG2a levels than Alum-adjuvanted ones. However, there were no significant differences between test and control groups regarding interferon-gamma (IFN-γ) levels, and the mice lung colonization experiments indicated that neither rOmpQ nor rLpp could confer protection alone against B . pertussis challenge.

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Cite This Article

YILMAZ, Ç., ÖZCENGİZ, E., ÖZCENGİZ, G. (2018). Recombinant outer membrane protein Q and putative lipoprotein from <i>Bordetella pertussis</i> inducing strong humoral response were not protective alone in the murine lung colonization model. Turkish Journal of Biology, Cilt: 42 - Sayı: 2, 123–131. https://ajindex.com/articles/722871

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