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Journals / The European Research Journal / 2022 / Cilt: 8 - Sayı: 6

Regulatory effects of laminin derived peptide on microtissue formation for tissue engineered scaffold-free constructs

Ziyşan Buse YARALI ÇEVİK
Ayşe ÖRDEK
Ozan KARAMAN
Pages
859–868
Publication Date
2022-11-04
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Thus, 1.5 mM YIGSR has been found as the most promising peptide concentration for increasing vascularization.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: Vascularization is an important stage for tissues and organs.
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Key Findings & Data:It is known that laminin-derived Tyr-Ile-Gly-Ser-Arg (YIGSR) peptide contributes to endothelial microtissue formation by promoting increased adhesion and proliferation of endothelial cells.
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Conclusion & Impact:Thus, 1.5 mM YIGSR has been found as the most promising peptide concentration for increasing vascularization.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Scaffold free microtissues · HUVEC microtissue formation · YIGSR peptide synthesis

Abstract

Objectives: Vascularization is an important stage for tissues and organs. The vascular network is succeeded by the attachment, spreading, proliferation of endothelial cells, and the completion of endothelialization. Endothelization can be mediated by laminin-derived peptides on microtissues. It is known that laminin-derived Tyr-Ile-Gly-Ser-Arg (YIGSR) peptide contributes to endothelial microtissue formation by promoting increased adhesion and proliferation of endothelial cells. This study aims to determine the efficacy of the laminin-derived YIGSR peptide in Human Umbilicial Vein Endothelial Cell (HUVEC) scaffold free microtissues (SFMs). Methods: After solid phase synthesis of YIGSR, microtissues were formed as SFMs. SFMs were cultured with 0 mM (control group), 1.5 mM and 3 mM YIGSR peptide. Diameters and viability analysis of HUVEC SFMs were performed on the 1st, 4th and 7th days. Results: The diameters of control SFMs group decreased day by day. Diameters of 3 mM YIGSR SFMs increased on the 1st and 4th days but significantly decreased on the 7th day. On the other hand, 1.5 mM YIGSR had a tendency on tissue formation because of increased diameter. As a result of the viability, YIGSR peptide increased cell viability. Conclusions: It has been determined that 1.5 mM YIGSR is the optimum amount for enlargement and viability of HUVEC SFMs. The concentration has contributed to proliferation and viability of endothelial SFMs. Thus, 1.5 mM YIGSR has been found as the most promising peptide concentration for increasing vascularization.

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Cite This Article

ÇEVİK, Z. B. Y., ÖRDEK, A., KARAMAN, O. (2022). Regulatory effects of laminin derived peptide on microtissue formation for tissue engineered scaffold-free constructs. The European Research Journal, Cilt: 8 - Sayı: 6, 859–868. https://ajindex.com/articles/703923

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References (15)

  1. Atala A, Bauer SB, Soker S, Yoo JJ, Retik AB. Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet 2006;367:1241-6
  2. Chen Y, Ma M, Teng Y, Cao H, Yang Y, Wang Y, et al. Efficient manufacturing of tissue engineered cartilage in vitro by a multiplexed 3D cultured method. J Mat Chem B 2020;8:2082-95
  3. Ali S, Saik JE, Gould DJ, Dickinson ME, West JL. Immobilization of cell-adhesive laminin peptides in degradable PEGDA hydrogels influences endothelial cell tubulogenesis. BioRes Open Access 2013;2:241-9
  4. Rouwkema J, Khademhosseini A. Vascularization and angiogenesis in tissue engineering: beyond creating static networks. Trends Biotechnol 2016;34:733-45
  5. Naito H, Iba T, Takakura N. Mechanisms of new blood-vessel formation and proliferative heterogeneity of endothelial cells. Inter Immunol 2020;32:295-305
  6. Park HJ, Zhang Y, Georgescu SP, Johnson KL, Kong D, Galper JB. Human umbilical vein endothelial cells and human dermal microvascular endothelial cells offer new insights into the relationship between lipid metabolism and angiogenesis. Stem Cell Rev 2006;2:93-101
  7. Kale S, Hanai JI, Chan B, Karihaloo A, Grotendorst G, Cantley LG, et al. Microarray analysis of in vitro pericyte differentiation reveals an angiogenic program of gene expression. FASEB J 2005;19:270-1
  8. Laranjeira M, Fernandes M, Monteiro F. Reciprocal induction of human dermal microvascular endothelial cells and human mesenchymal stem cells: time‐dependent profile in a co‐culture system. Cell Prolif 2012;45:320-34
  9. Duval K, Grover H, Han L-H, Mou Y, Pegoraro AF, Fredberg J, et al. Modeling physiological events in 2D vs. 3D cell culture. Physiology (Bethesta) 2017;32:266-77
  10. Fang X, Sittadjody S, Gyabaah K, Opara EC, Balaji KC. Novel 3D co-culture model for epithelial-stromal cells interaction in prostate cancer. PloS One 2013;8:e75187
  11. Baker BM, Chen CS. Deconstructing the third dimension-how 3D culture microenvironments alter cellular cues. J Cell Sci 2012;125:3015-24
  12. Antoni D, Burckel H, Josset E, Noel G. Three-dimensional cell culture: a breakthrough in vivo. Int J Mol Sci 2015;16:5517-27
  13. Zhang W, Zhuang A, Gu P, Zhou H, Fan X. A review of the three-dimensional cell culture technique: approaches, advantages and applications. Curr Stem Cell Res Ther 2016;11:370-80
  14. Iorio V, Troughton LD, Hamill KJ. Laminins: roles and utility in wound repair. Adv Wound Care (New Rochelle) 2015;4:250-63
  15. Walker C, Mojares E, Del Río Hernánde