SavedAdd your journal

Journals / Tarım Bilimleri Dergisi / 2022 / Cilt: 28 - Sayı: 4

Genoprotective Role of Purslane Methanol Extract Against Somatic Mutations Induced by Bifenthrin, a Third Generation Prethyroid Insecticide

Halit KIZILET
Handan UYSAL
Pages
583–591
Publication Date
2022-10-17
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Some of the components found in the highest ratio in this extract are γ-sitosterol (21.86%), 13-docosenamide, (13.30%), palmitic acid (12.85%), stigmasteol (6.64%), campesterol (5.69%), linoleicacid (5.46%) and 2-methyl-1-hexadecanol (3.88%).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, in vitro and in vivo genotoxic effects of Bifenthrin (BIF), an important insecticide used in agricultural production, storage and processing, were investigated.
📊
Key Findings & Data:Later, another experiment was conducted and it was determined that the genotoxic effects of BIF were reduced with POmet (1:1 v/v).
💡
Conclusion & Impact:Some of the components found in the highest ratio in this extract are γ-sitosterol (21.86%), 13-docosenamide, (13.30%), palmitic acid (12.85%), stigmasteol (6.64%), campesterol (5.69%), linoleicacid (5.46%) and 2-methyl-1-hexadecanol (3.88%).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Drosophila melanogaster · Portulaca oleracea · Household insecticides

Abstract

In this study, in vitro and in vivo genotoxic effects of Bifenthrin (BIF), an important insecticide used in agricultural production, storage and processing, were investigated. The genoprotective properties of purslane (Portulaca oleracea L.) against the genotoxic effects of BIF were also determined by using the methanol (POmet) extract of this plant. In vivo experiments were performed with somatic mutation and recombination test (SMART) in Drosophila melanogaster. In in vitro studies, human peripheral blood cultures were prepared and different concentrations of BIF were applied to lymphocyte cells in accordance with the procedure of both the micronucleus (MN) and sister chromatid exchange (SCE) assay. The results obtained from all applied tests showed that BIF is genotoxic and induces chromosomal mutations. Later, another experiment was conducted and it was determined that the genotoxic effects of BIF were reduced with POmet (1:1 v/v). This result, which was observed in all in vivo and in vitro tests, shows that purslane plant is a potent radical scavenger. Due to the healing properties of POmet, gas chromatography-mass spectrometry (GS-MS) method was used to determine the components in its content. Some of the components found in the highest ratio in this extract are γ-sitosterol (21.86%), 13-docosenamide, (13.30%), palmitic acid (12.85%), stigmasteol (6.64%), campesterol (5.69%), linoleicacid (5.46%) and 2-methyl-1-hexadecanol (3.88%).

🔖

Cite This Article

KIZILET, H., UYSAL, H. (2022). Genoprotective Role of Purslane Methanol Extract Against Somatic Mutations Induced by Bifenthrin, a Third Generation Prethyroid Insecticide. Tarım Bilimleri Dergisi, Cilt: 28 - Sayı: 4, 583–591. https://ajindex.com/articles/702543

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Arora A, Sairam R K & Srivastava G C (2002). Oxidative stress and antioxidative systems in plants. Current Science 82: 1227-1238
  2. Aydın S (2009). The identifıcation of antioxidant, antimicrobial effects and fatty acids composition of Terfezia boudieri chatin and Lactarius vellereus (Fr.). Master thesisi, Selçuk University, TR Balamurugan R, Duraipandiyan V & Ignacimuthu S (2011). Antidiabeticactivity of γ-sitosterol isolated from Lippia nodiflora L. in streptozotocin induced diabetic rats. European Journal of Pharmacology 667(1-3): 410-418
  3. Behravan J, Mosafa F, Soudmand N, Taghiabadi E, Razavi B M & Karimi G (2011). Protectiv eeffects of aqueous and ethanolic extracts of Portulaca oleracea L. Aerial parts on H2O2- induced DNA damage in Lymphocytes by comet assay. Journal of Acupuncture and Meridian Studies 4: 193-197
  4. Bhuvaneswari V, Phil M, Abraham S K & Nagini S (2005). Combinatorial antigenotoxic and anticarcinogenic effects of tomato and garlic through modulation of xenobiotic-metabolizing enzymes during hamster buccal pouch carcinogenesis. Nutrition 21: 726-731 Birch‐Machin M A & Bowman A (2016). Oxidative stres and ageing. British Journal of Dermatology 175: 26-29
  5. Cantelli-Forti G, Paolini M & Hrelial P (1993). Multiple end point procedure to evaluate risk from pesticides. Environmental Health Perspectives 101: 15-20
  6. Costa L G (2015). The neurotoxicity of organochlorine and pyrethroid pesticides. Handbook of Clinical Neurology 131: 135-48
  7. Demirci M, Hiller K A, Boslb C, Gallerb K, Schmalzb G & Schweiklb H (2008). The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives. Dental Materials 24: 362-371
  8. Demsia G, Vlastosa D, Goumenoub M & Matthopoulos D P (2007). Assessment of the genotoxicity of imidacloprid and metalaxyl in cultured human lymphocytes and rat bone-marrow. Mutation Research/Genetic Toxicology and Environmental 634: 32-39
  9. Dev S (2017). Insecticides of natural origin.1st Edition. Available on Taylor & Francis eBooks, Routledge, London. Durmuşoğlu E, Tiryaki O & Canhilal R (2010).
  10. Türkiye'de Pestisit Kullanımı, Kalıntı ve Dayanıklılık Sorunları, In: VII. Türkiye Ziraat Mühendisliği Teknik Kongresi, 11-15
  11. Ocak, Ankara, Bildiriler Kitabı 2: pp. 589-607
  12. El-Nekeety A A, Abdel-Wahhab K G, Abdel-Aziem S H, Mannaa F A, Hassan N S & Abdel-Wahhab M A (2017). Papaya fruits extracts enhance the antioxidant capacity and modulate the genotoxicity and oxidative stress in the kidney of rats fed ochratoxin A-contaminated diet. Jou