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Journals / Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi / 2021 / Cilt: 12 - Sayı: 2

Investigation of The Stability of 177LuPSMA-I&T Prostate-specific Membrane Antigen İnhibitor Used in The Treatment of Castration Resistant Prostate Cancer

Kastrasyona Dirençli Prostat Kanseri Tedavisinde Kullanılan 177LuPSMA-I&T’nin İnvitro Stabilitesinin İncelenmesi

Ayşe UĞUR
Aziz GÜLTEKİN
Pages
227–232
Publication Date
2021-08-20
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusions: With the easy and fast labeled 177LuPSMA-I&T radiopharmaceutical is safe to use in treatment due to its stability in the first 6 hours.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Internalization of the prostate-specific membrane antigen (PSMA) by ligand binding through clathrin-coated vesicle followed by endocytosis provides it an excellent target in prostate cancer imaging Gallium-68 (68Ga) and treatment Lutetium-177 (177Lu).
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Key Findings & Data:The purpose of this research is to examine the in-vitro stability of the radiopharmaceutical (177LuPSMA-I&T) labeled with 177Lu of PSMA-I&T in saline.Material-Method: Labeling of PSMA-I&T with 177Lu was carried out in the automated synthesis module.
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Conclusion & Impact:Conclusions: With the easy and fast labeled 177LuPSMA-I&T radiopharmaceutical is safe to use in treatment due to its stability in the first 6 hours.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Prostata spesifik membran antijeni · PSMA · 177LuPSMA-I&T

Abstract

Objective: Internalization of the prostate-specific membrane antigen (PSMA) by ligand binding through clathrin-coated vesicle followed by endocytosis provides it an excellent target in prostate cancer imaging Gallium-68 (68Ga) and treatment Lutetium-177 (177Lu). The purpose of this research is to examine the in-vitro stability of the radiopharmaceutical (177LuPSMA-I&T) labeled with 177Lu of PSMA-I&T in saline.Material-Method: Labeling of PSMA-I&T with 177Lu was carried out in the automated synthesis module. The labeled 200mCi (7.4 GBq) 177LuPSMA-I&T was incubated at 37oC for 72 hours. Samples taken at regular intervals up to 72 hours were analyzed by radio-high performance liquid chromatography (RP-HPLC). Results: The radiochemical efficiency of 177LuPSMA-I&T was > 99%. We determined that 177LuPSMA-I&T prepared with PSMA-I&T remained stable up to 6 hours, and deteriorated by 3.1% at the 24th hour. Conclusions: With the easy and fast labeled 177LuPSMA-I&T radiopharmaceutical is safe to use in treatment due to its stability in the first 6 hours.

Özet

Giriş: Prostat spesifik membran antijeni (PSMA), klatrin kaplı veziküller yoluyla hücreye bağlanır ve ardından endositoz yoluyla hücre içine girer. Bu özelliği nedeniyle, PSMA prostat kanseri görüntülemede Galyum-68 (68Ga) ve tedavide Lutesyum-177 (177Lu) ile işaretlenebilen mükemmel bir moleküldür. Çalışmanın amacı, PSMA-I&T'nin 177Lu ile işaretlenmiş radyofarmasötiğinin (177LuPSMA-I&T) serum fizyolojik içerisinde in-vitro stabilitesini incelemektir. Materyal-Metod: PSMA-I&T'nin 177Lu ile işaretlemesi otomatik sentez modulünde gerçekleştirildi. İşaretlenmiş 200mCi (7,4 GBq) 177LuPSMA-I&T 37oC'de 72 saat inkübasyona bırakıldı. 72. saate kadar belirli aralıklarla alınan örnekler radyo-yüksek performanslı sıvı kromatografisi (RP-HPLC) ile analiz edildi. Bulgular: 177LuPSMA-I&T'nin radyokimyasal verimi >% 99 bulundu. PSMA-I&T ile hazırlanan 177LuPSMA-I&T'nin ilk 6 saate kadar stabil kaldığı, 24. saatte ise stabilitesinde %3,3 oranında bozunumun başladığı, 48. saatten sonra ise kullanıma uygun olmadığı belirlendi. Sonuç: 177LuPSMA-I&T ile tedavide radyofarmasötiğin ilk 6.saatteki stabilitesi nedeniyle görüntüleme ve tedavide kullanımının daha güvenli olduğu, gerektiğinde etiketlemeden sonraki 48 saate kadar kullanılabileceği sonucuna varılmıştır.

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Cite This Article

UĞUR, A., GÜLTEKİN, A. (2021). Investigation of The Stability of 177LuPSMA-I&T Prostate-specific Membrane Antigen İnhibitor Used in The Treatment of Castration Resistant Prostate Cancer. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi, Cilt: 12 - Sayı: 2, 227–232. https://ajindex.com/articles/702267

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