Journals / Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2011 / Cilt: 15 - Sayı: 1

Determination of Glycoconjugates in Helix lucorum’s Salivary Gland by Lectin Histochemistry

Helix lucorum'un Tükürük Bezindeki Glikokonjugatların Lektin Histokimyası ile Belirlenmesi

Pages
46–50
Publication Date
2014-07-17
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Type 3, 4 ve 5 cells did not showed reaction aganist the any of the applied lectins.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study taking into acconut the size and morphological features, the five types of cells were determinated in salivary glands of Helix lucorum.
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Key Findings & Data:These cells were called as type 1, 2, 3, 4 and 5.
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Conclusion & Impact:Type 3, 4 ve 5 cells did not showed reaction aganist the any of the applied lectins.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Helix lucorum · Tükürük Bezi · Glikokonjugat

Abstract

In this study taking into acconut the size and morphological features, the five types of cells were determinated in salivary glands of Helix lucorum. These cells were called as type 1, 2, 3, 4 and 5. As a result of the counts it was idendified that type 1 cell was the highest number and type 3 cell was the lowest number. As a result of the measuring the diameters of the cells it was detected that the largest cell was type 1 and the smallest cell was type 3. Type 1 and 2 cells showed the pozitive reaksiyon aganist the all of the applied lectins that Dolichus biflorus (DBA), Ulex europaeus (UEA-1), Arachis hypogaea (PNA), Canavalia ensiformis (Con A), Bandeiraea simplicifolia (BSA I-B4), Triticum vulgare (WGA). While it was observed that these two types cells were given the weak reaction aganist the BSA I-B4, the strong reaction aganist PNA and DBA. Type 3, 4 ve 5 cells did not showed reaction aganist the any of the applied lectins.

Özet

Bu çalışmada Helix lucorum'un tükürük bezinde büyüklük ve morfolojik özellikleri dikkate alınarak beş hücre tipi tespit edildi. Bu hücreler Tip 1, 2, 3, 4 ve 5 olarak isimlendirildi. Yapılan sayımlar sonucunda tükürük bezinde en fazla Tip 1, en az Tip 3 hücrelerinin bulunduğu belirlendi. Hücrelerin çaplarının ölçülmesi sonucu en büyük hücrenin Tip 1, en küçük hücrenin Tip 3 olduğu saptandı. Tip 1 ve 2 hücrelerinin uygulanan Dolichos biflorus (DBA), Ulex europaeus (UEA-1), Arachis hypogaea (PNA), Canavalia ensiformis (Con A), Bandeiraea simplicifolia (BSA I-B4), Triticum vulgare (WGA) lektinlerinin hepsine karşı reaksiyon verdiği belirlendi. Bu iki hücre tipinin BSA I-B4 ile zayıf reaksiyon verdiği gözlenirken, PNA ve DBA ile kuvvetli reaksiyon verdiği saptandı. Tip 3, 4 ve 5 hücrelerinin ise uygulanan hiçbir lektine karşı reaksiyon göstermediği belirlendi.

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Cite This Article

ZORLU, S., DEMİRBAĞ, E., ÇINAR, K. (2014). Determination of Glycoconjugates in Helix lucorum’s Salivary Gland by Lectin Histochemistry. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 15 - Sayı: 1, 46–50. https://ajindex.com/articles/701527

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