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Journals / Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2018 / Cilt: 22 Sayı: Özel

Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles

Dilek ODACI DEMIRKOL
Kadri GULEC
Pages
421–426
Publication Date
2018-10-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Finally, cell analysis was carried out in real samples.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aptamers are selective molecules against to various sizes of targets from small molecules to mammalian cells.
📊
Key Findings & Data:Here, we reported QDs containing electrochemical aptasensor for the detection of E. coli O157:H7.
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Conclusion & Impact:Finally, cell analysis was carried out in real samples.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:E. coli O157:H7 sensing · Electrochemical detection · Fluorescence imaging

Özet

Aptamers are selective molecules against to various sizes of targets from small molecules to mammalian cells. Here, we reported QDs containing electrochemical aptasensor for the detection of E. coli O157:H7. The electrode surfaces were modified by cysteamine (CysN), which has amine and thiol groups, via self-assembled monolayer formation. The carboxyl-functionalized quantum dots (QD) and aptamers (Apt) were conjugated to cysteamine modified gold electrodes. Linear range for E. coli O157:H7 was from 1 to 102 CFU/mL after incubation on CysN/QD/Apt modified Au surfaces. QDs provide fluorescence surface, so that adhesion of cells was followed using fluorescence microscope. Adhered cells were also imaged by scanning electron microscopy. Finally, cell analysis was carried out in real samples.

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Cite This Article

DEMIRKOL, D. O., GULEC, K. (2018). Electrochemical sensing and fluorescence imaging of E. coli O157:H7 based on aptamer-conjugated semiconducting nanoparticles. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 22 Sayı: Özel, 421–426. https://ajindex.com/articles/701136

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