SavedAdd your journal

Journals / Pamukkale Tıp Dergisi / 2022 / Cilt: 15 - Sayı: 4

Comparison of serum and lesional miRNA-1291 expressions in patients with bullous pemphigoid

Büllöz pemfigoidli hastalarda serum ve lezyonel miRNA-1291 ekspresyonlarının karşılaştırılması

Şule SUBAŞI GÖKŞİN
Buket ER URGANCI
Işıl Göğem İMREN
İbrahim AÇIKBAŞ
Pages
836–844
Publication Date
2022-10-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As miRNA-1291 correlates with other BP biomarkers, it may serve as an important biomarker for BP.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Purpose: Bullous pemphigoid (BP) is an autoimmune disease increasing by age. miRNA-1291 is a microRNA that plays major role in many immune-mediated cutaneous diseases.
📊
Key Findings & Data:We aimed to investigate the role of miRNA-1291 in BP by comparing its expression levels in serum and lesional skin and its correlation with main BP biomarkers (anti-BP180, and anti-BP230) and CCL17Materials and methods: Twenty three patients with BP and 23 healthy control patients were included in the study.
💡
Conclusion & Impact:As miRNA-1291 correlates with other BP biomarkers, it may serve as an important biomarker for BP.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Büllöz pemfigoid · biyobelirteç · miRNA-1291

Abstract

Purpose: Bullous pemphigoid (BP) is an autoimmune disease increasing by age. miRNA-1291 is a microRNA that plays major role in many immune-mediated cutaneous diseases. We aimed to investigate the role of miRNA-1291 in BP by comparing its expression levels in serum and lesional skin and its correlation with main BP biomarkers (anti-BP180, and anti-BP230) and CCL17Materials and methods: Twenty three patients with BP and 23 healthy control patients were included in the study. The miRNeasy Mini Kits was used for RNA isolation from serum and biopsy samples. Qiagen-miRCURY LNA RT Kit was used for conversion of cDNA. miRNA-1291 expression was performed by Real-Time PCR with the original primer and SYBR Green master mix. Serum CCL17, anti-BP180, and anti-BP230 levels were measured with use of ELISA. SPSS 25.0 (IBM SPSS Statistics 25 software) was used for statistical evaluation.Results: The expression of miRNA-1291 in serum and biopsy samples were 7.02 and 4.77-fold significantly upregulated than that of control group respectively (p=0.001, p=0.007). The serum levels of anti-BP-180, anti-BP-230 and CCL17 in BP patients were significantly higher compared to control group (p=0.0049, p=0.0071, p=0.001). Anti-BP180, anti-BP230, CCL17 levels and serum miRNA-1291 expression were correlated positively in BP patients. Additionally, serum miRNA-1291 expression were correlated well with lesional miRNA-1291 expression.Conclusion: The increase in miRNA-1291 levels in serum and lesional tissue compared to non-lesional tissue suggests that miRNA-1291 may play a role in the pathogenesis of BP. As miRNA-1291 correlates with other BP biomarkers, it may serve as an important biomarker for BP.

Özet

Amaç: Büllöz pemfigoid (BP), yaşla birlikte artan otoimmün bir hastalıktır. miRNA-1291, birçok immün aracılı kutanöz hastalıkta önemli rol oynayan bir mikroRNA'dır. Serum ve lezyonlu derideki ekspresyon seviyelerini ve ana BP biyobelirteçleri (anti-BP180 ve anti-BP230) ve CCL17 ile korelasyonunu karşılaştırarak miRNA-1291'in BP'deki rolünü araştırmayı amaçladık.Gereç ve yöntem: BP'li 23 hasta ve 23 sağlıklı kontrol hastası çalışmaya dahil edildi. Serum ve biyopsi örneklerinden RNA izolasyonu için miRNeasy Mini Kitleri kullanıldı. cDNA'nın dönüştürülmesi için Qiagen-miRCURY LNA RT Kiti kullanıldı. miRNA-1291 ekspresyonu, orijinal primer ve SYBR Green ana karışımı ile Real-Time PCR ile gerçekleştirildi. Serum CCL17, anti-BP180 ve anti-BP230 seviyeleri ELISA kullanılarak ölçüldü. İstatistiksel değerlendirme için SPSS 25.0 (IBM SPSS Statistics 25 yazılımı) kullanıldı.Bulgular: Serum ve biyopsi örneklerinde miRNA-1291 ekspresyonu, kontrol grubuna göre sırasıyla 7.02 ve 4.77 kat anlamlı şekilde up-regüleydi (p=0,001, p=0.007). BP ve CCL17 hastalarında anti-BP-180, anti-BP-230 serum düzeyleri kontrol grubuna göre anlamlı derecede yüksekti (p=0,0049, p=0,0071, p=0,001). BP hastalarında anti-BP180, anti-BP230, CCL17 seviyeleri, serum miRNA-1291 ekspresyonu ile pozitif korelasyon gösterdi. Ek olarak, serum miRNA-1291 ekspresyonu, lezyonel miRNA-1291 ekspresyonu ile iyi korele idi.Sonuç: Serum ve lezyonlu dokuda lezyonlu olmayan dokuya göre miRNA-1291 düzeylerinin artması, miRNA-1291'in BP'nin patogenezinde rol oynayabileceğini düşündürmektedir. miRNA-1291 diğer BP biyobelirteçleri ile korelasyon gösterdiğinden, BP için önemli bir biyobelirteç olarak hizmet edebilir.

🔖

Cite This Article

GÖKŞİN, Ş. S., URGANCI, B. E., İMREN, I. G., AÇIKBAŞ, İ. (2022). Comparison of serum and lesional miRNA-1291 expressions in patients with bullous pemphigoid. Pamukkale Tıp Dergisi, Cilt: 15 - Sayı: 4, 836–844. https://ajindex.com/articles/696354

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Zhao CY, Murrell DF. Outcome measures for autoimmune blistering diseases. J Dermatol 2015;42:31-36.
  2. Sonkoly E, Ståhle M, Pivarcsi A. MicroRNAs: novel regulators in skin inflammation. Clin Exp Dermatol 2008;33:312-315.
  3. Iorio MV, Croce CM. MicroRNA dysregulation in cancer: Diagnostics, monitoring and therapeutics. A comprehensive review. EMBO Mol Med 2012;4:143-159. https://doi.org/10.1002/emmm.201100209. Epub 2012 Feb 20. Erratum in: EMBO Mol Med 2017;9:852
  4. Chen JQ, Papp G, Szodoray P, Zeher M. The role of microRNAs in the pathogenesis of autoimmune diseases. Autoimmun Rev 2016;15:1171-1180.
  5. Genovese G, Di Zenzo G, Cozzani E, Berti E, Cugno M, Marzano AV. New ınsights into the pathogenesis of bullous pemphigoid: 2019 Update. Front Immunol 2019;10:1506.
  6. Chiorean RM, Baican A, Mustafa MB. et al. Complement-activating capacity of autoantibodies correlates with disease activity in bullous pemphigoid patients. Front Immunol 2018;9:2687.
  7. Amber KT, Murrell DF, Schmidt E, Joly P, Borradori L. Autoimmune subepidermal bullous diseases of the skin and mucosae: Clinical features, diagnosis, and management. Clin Rev Allergy Immunol 2018;54:26-51.
  8. Liu Y, Li L, Xia Y. BP180 is critical in the autoimmunity of bullous pemphigoid. Front Immunol 2017;8:1752.
  9. Feliciani C, Caldarola G, Kneisel A. et al. IgG autoantibody reactivity against bullous pemphigoid (BP) 180 and BP230 in elderly patients with pruritic dermatoses. Br J Dermatol 2009;161:306-312.
  10. Qiu L, Zhang L, Qi R, Gao X, Chen H, Xiao T. miR-1291 Functions as a potential serum biomarker for bullous pemphigoid. Dis Markers 2020;2020:9505312.
  11. Marazza G, Pham HC, Schärer L. et al. Autoimmune bullous disease Swiss study group. Incidence of bullous pemphigoid and pemphigus in Switzerland: a 2-year prospective study. Br J Dermatol 2009;161:861-868.
  12. Bakay ÖSK. Kronik spontan ürtiker ile microrna-221, microrna-155 ve IL-31 ilişkisinin araştırılması - Investigation of relationship between chronic spontaneous urticaria with microrna-221, micorna-155 and IL-31. htt