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Journals / Middle East Journal of Science / 2016 / Cilt: 2 - Sayı: 1

THE PREPARATION OF A NEW PROTEIN BASED HPLC COLUMN PACKING MATERIAL AND ENANTIOMERIC RESOLUTION OF SOME PHARMACEUTICAL RELATED COMPOUNDS VIA THIS COLUMN

Ömer ERDOĞAN
Giray TOPAL
Reşit ÇAKMAK
Murat SÜNKÜR
Mutlu CANPOLAT
Pages
77–92
Publication Date
2016-06-29
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Chromatographic parameters such as Rs and α were calculated for each resoluted compounds.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, BSA was bounded to 3-chloropropyl functionalized silica gel via ethylenediamine and glutaric dialdehyde.
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Key Findings & Data:The prepared CSP was packed into 150X4.6mm HPLC column according to slurry method.
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Conclusion & Impact:Chromatographic parameters such as Rs and α were calculated for each resoluted compounds.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Enantiomeric separation · hplc · bovine serum albumin

Abstract

In this study, BSA was bounded to 3-chloropropyl functionalized silica gel via ethylenediamine and glutaric dialdehyde. The prepared CSP was packed into 150X4.6mm HPLC column according to slurry method. The enantiomeric resolution of some pharmaceutical related compounds such as rac-mandelic acid, rac-methyl mandelate, rac-epinephrine, rac-propranolol, rac-α-phenylethylamine and rac-3-hydroxybutyric acid was employed in 1mL/rpm, 1,5mL/rpm, 2mL/rpm, 2,5mL/rpm, 3mL/rpm flow rate in various pHs such pH=6, pH=7, pH=8 0,1M phosphate buffer. Chromatographic parameters such as Rs and α were calculated for each resoluted compounds.

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Cite This Article

ERDOĞAN, Ö., TOPAL, G., ÇAKMAK, R., SÜNKÜR, M., CANPOLAT, M. (2016). THE PREPARATION OF A NEW PROTEIN BASED HPLC COLUMN PACKING MATERIAL AND ENANTIOMERIC RESOLUTION OF SOME PHARMACEUTICAL RELATED COMPOUNDS VIA THIS COLUMN. Middle East Journal of Science, Cilt: 2 - Sayı: 1, 77–92. https://ajindex.com/articles/691592

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