SavedAdd your journal

Journals / Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online) / 2022 / Cilt: 6 - Sayı: 3

Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation

Hayat Topçu
Pages
205–217
Publication Date
2022-11-11
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The survival rate of plants transferred to ex vitro conditions was 100%.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Rubus spp. is a shrub-form plant known for its fruits called blackberries.
📊
Key Findings & Data:Proliferation from axillary buds was made by adding BAP (6- Benzylaminopurine), NAA (Naphthalinacetic acid) and GA3 (Gibberellic acid) in various combinations and concentrations to the MS medium.
💡
Conclusion & Impact:The survival rate of plants transferred to ex vitro conditions was 100%.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Rubus spp. is a shrub-form plant known for its fruits called blackberries. Blackberries are plants with high commercial value, delicious taste, nice aroma, and high nutritional value. Turkey has wealthy genetic origins of Rubus species. Conventionally, the trading propagation of Rubus plants is done as vegetatively, utilizing truncation, rooting, or stratuming. However, these traditional methods are time-consuming and inefficient in virus-free plant production. Cloning of plant grown in the tissue culture also enables to obtain virus-free plants and to provide fast replicating high standard plants. Rubus obtained by micropropagation is used for the formation of commercial fruit plantations as well as source plant formation. In this work, the aim is the development of in vitro micropropagation process of the wild Rubus in the Trakya Region. Proliferation from axillary buds was made by adding BAP (6- Benzylaminopurine), NAA (Naphthalinacetic acid) and GA3 (Gibberellic acid) in various combinations and concentrations to the MS medium. Rooting was successfully realized with 83.3% rooted plants in 1 IBA medium. No roots were seen in 0 MS. The survival rate of plants transferred to ex vitro conditions was 100%.

🔖

Cite This Article

Topçu, H. (2022). Optimal Propagation and Rooting Mediums in Rubus spp. by in Vitro Micropropagation. Uluslararası tarım araştırmalarında yenilikçi yaklaşımlar dergisi (Online), Cilt: 6 - Sayı: 3, 205–217. https://ajindex.com/articles/655504

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Aguilera-Arango, G.A., Gómez-López, E.D. & González-Mejia. A. (2019). Callogénesisen cultivares híbridos de Cocos nucifera L. mediante cultivo in vitro de inflorescencias in maduras. Biotecnología Vegeta, l 19(4):277-284.
  2. Akin, M., Eyduran, S.P., Ercisli, S., Toteva, V.K. & Eyduran, E. (2016). Phytochemical profiles of wild blackberries, black and white mulberries fr.om southern Bulgaria. Biotechnology & Biotechnological Equipment, 30:899-906.
  3. Andrade, A., Gómez, L., Torres, Y. & Aguilera-Arango., G. (2021). Evaluation Of Growing Media for the In Vitro Establishment, Multiplication and Rooting of Blackberry (Rubus glaucusBenth.). Chilean J. Agric. Anim. Sci., ex Agro-Ciencia, 37(2):117-127.
  4. Bite, A. & Petrevica, L. (2002). The influence of in vitro propagation on the field behaviour of red raspberry variety ‘Norna’. Acta Hortic., 585, 615–619.
  5. Bobrowski, V. L., Mello-Farias, P. & Peters, C.P. (1996). Micropropagation of blackberries (Rubus sp.) cultivars. Revista Brasileira de Agrociencia 2:17-20 (in Italian). Borodulina, I.D., Plaksina, T.V., Panasenko, V.N. & Sokolova, G.G. (2019). Оptimization of blackberry clonal micropropagation. Ukrainian Journal of Ecology, 9(3):339-345.
  6. Boxus, P., Damiano, C. & Brasseur, E. (1989). Strawberry. In: Ammirato P, Evans d, Sharp W, Yamada Y (Eds). Handbook of plant cell culture. New York, Macmillan pp 453-486.
  7. Broome, O.C. & Zimmerman, R.H. (1978). Invitro propagation of blackberry. HortScience, 13:151-153.
  8. Caldwell, J.D. (1984). Blackberry propagation. HortScience, 2:193-195.
  9. Cancino-Escalante, G.O., Sánchez-Montaño, L.R., Quevedo-García, E. & Díaz-Carvajal, C. (2011). Caracterización fenotípica de accesiones de especies de Rubus L. de los municipios de Pamplona y Chitagá, región Nororiental de Colombia. Universitas Scientiarum 16(3):219-233.
  10. Cao, X &, Hammerschlag, F.A. (2000). Improved shoot organogenesis from leaf explants of highbush blueberry. HortScience, 35:945-947.
  11. Cousineau, J.C. & Donnelly, D.J. (1991). Adventitious shoot regeneration from leaf explants of tissue cultured and greenhouse-grown raspberry. Plant Cell Tissue Organ Culture, 27:249-255.
  12. De Oliveira, R.P. & Nino, A.F.P. (2009). In vıtro multiplication rate of raspberry cultıvars. Rev. Bras. Frutic., Jaboticabal - SP, 31,1, 280-284 (in Portuguese). Debnath, S.C. (2004). Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation. AGRIS. 43(2):179-186. ISSN: 0167-6903.
  13. Demenko, V.I., Shestibratov, K.A. &