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Journals / Turkish Journal of Urology / 2017 / Cilt: 43 - Sayı: 4

Protective role of resveratrol on testicular germ cells in mice with testicular toxicity

Resveratrolün testis toksisitesi oluşturulmuş farelerde testiküler germ hücreleri üzerine koruyucu etkisi

Fikriye Yasemin ÖZATİK
Kevser EROL
Orhan ÖZATİK
Çiğdem Çengelli ÜNEL
Semra YİĞİTASLAN
Pages
444–450
Publication Date
2022-11-04
DOI
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Serum testosterone, LH and FSH levels were measured by a commerciallyavailable ELISA kit.Results: Resveratrol caused a dose-dependent increase in TW/BW and decrease in TDW/TWW (p

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Objective & Hypothesis:Objective: The aim of the present study was to investigate the possible beneficial effects of resveratrol in micesubjected to vinyl cyclohexene dieposide (VCD) -induced testicular toxicity.Material and methods: A total of thirty- six Swiss albino male mice aged 28-days were used in the presentstudy.
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Key Findings & Data:The study was composed of two stages where mice which received or did not receive VCD (320 mg/kg/day) were administered resveratrol.
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Conclusion & Impact:Serum testosterone, LH and FSH levels were measured by a commerciallyavailable ELISA kit.Results: Resveratrol caused a dose-dependent increase in TW/BW and decrease in TDW/TWW (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of the present study was to investigate the possible beneficial effects of resveratrol in micesubjected to vinyl cyclohexene dieposide (VCD) -induced testicular toxicity.Material and methods: A total of thirty- six Swiss albino male mice aged 28-days were used in the presentstudy. The study was composed of two stages where mice which received or did not receive VCD (320 mg/kg/day) were administered resveratrol. The animals were assigned into control and resveratrol-treated groups inthe first stage and into groups of VCD- and VCD+resveratrol-treated groups in the second stage. At the end ofthe experiments, relative testicular weight (TW/BW) and dry/wet weight of testis (TDW/TWW) were calculated.Histological analysis by hematoxylin and eosin (H&E) staining and immunohistochemical staining byBAX and Bcl-2 were performed. Serum testosterone, LH and FSH levels were measured by a commerciallyavailable ELISA kit.Results: Resveratrol caused a dose-dependent increase in TW/BW and decrease in TDW/TWW (p

Özet

Amaç: Bu çalışmada amaç vinil sikloheksen diepoksid (VCD) ile testiküler toksisite oluşturulmuş farelerde resveratrolün olası faydalı etkilerini incelemektir. Gereç ve yöntemler: Çalışmada 28 günlük toplam otuz altı adet Swiss Albino erkek fare kullanıldı. Çalışma 320 mg/kg/gün VCD ile ve VCD kullanılmadan resveratrol verilerek iki aşamada oluşturuldu. İlk aşamada gruplar kontrol ve resveratrol tedavi grubu, ikinci aşamada ise VCD ve VCD+ resveratrol grupları olarak belirlendi. Tedavinin sonunda testis ağırlığı/ vücut ağırlığı oranları (TA/VA), testis yaş/kuru ağırlığı oranları (TKA/TYA) hesaplandı. BAX ve Bcl2 uygulanarak immunohistokimyasal boyama ve Hematoksilen ve Eozin (H&E) boyama uygulanarak histolojik analiz yapıldı. Serum testesteron, FSH ve LH seviyeleri uygun ticari ELİSA kit ile ölçüldü. Bulgular: Resveratrol doz bağımlı olarak TA/VA oranlarını artırmış, TKA/TYA oranlarını azaltmıştır (p

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Cite This Article

ÖZATİK, F. Y., EROL, K., ÖZATİK, O., ÜNEL, Ç. Ç., YİĞİTASLAN, S. (2022). Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. Turkish Journal of Urology, Cilt: 43 - Sayı: 4, 444–450. https://ajindex.com/articles/654597

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