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Journals / Turkish Journal of Biology / 2016 / Cilt: 40 - Sayı: 6

Cloning of two basic helix-loop-helix transcription factor genes from Tartary buckwheat (Fagopyrum tataricum) and their expression under abiotic stress

Xiao-Peng LUO
Hai-Xia ZHAO
Sang-Un PARK
Hui CHEN
Cheng-Lei LI
Jun XUE
Qi WU
Pages
1192–1201
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Additionally, FtbHLH2 showed a more intense response to jasmonic acid treatment than FtbHLH1.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Basic helix-loop-helix (bHLH) transcription factors are one of the most important transcription factors involved in abiotic stress tolerance in Tartary buckwheat.
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Key Findings & Data:In this study, we successfully isolated two bHLH transcription factor genes, designated as FtbHLH1 and FtbHLH2, from Tartary buckwheat.
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Conclusion & Impact:Additionally, FtbHLH2 showed a more intense response to jasmonic acid treatment than FtbHLH1.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Basic helix-loop-helix (bHLH) transcription factors are one of the most important transcription factors involved in abiotic stress tolerance in Tartary buckwheat. In this study, we successfully isolated two bHLH transcription factor genes, designated as FtbHLH1 and FtbHLH2, from Tartary buckwheat. Their encoding proteins were found to have a motif to interact with R2R3-MYB at the N-terminus and a typical bHLH motif at the C-terminus. Yeast one-hybrid assay proved that the two bHLH proteins exhibited obvious transcriptional activation activities. The abiotic stresses indicated that FtbHLH1 expression would be mainly affected by drought and UV-B, whereas FtbHLH2 expression would present a sensitive response to cold and UV-B. Additionally, FtbHLH2 showed a more intense response to jasmonic acid treatment than FtbHLH1.

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Cite This Article

LUO, X., ZHAO, H., PARK, S., CHEN, H., LI, C., XUE, J., WU, Q. (2022). Cloning of two basic helix-loop-helix transcription factor genes from Tartary buckwheat (Fagopyrum tataricum) and their expression under abiotic stress. Turkish Journal of Biology, Cilt: 40 - Sayı: 6, 1192–1201. https://ajindex.com/articles/654446

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References (11)

  1. Bai YC, Li CL, Zhang JW, Li SJ, Luo XP, Yao HP, Chen H, Zhao HX, Park SU, Wu Q (2014). Characterization of two tartary buckwheat R2R3‐MYB transcription factors and their regulation of proanthocyanidin biosynthesis. Physiol Plantarum 152: 431-440.
  2. Baloğlu MC, Zakharov FN, Öktem HA, Yücel AM (2011). Molecular cloning, characterization, and expression analysis of a gene encoding a Ran binding protein (RanBP) in Cucumis melo L. Turk J Biol 35: 387-397.
  3. Barcelo J, Poschenrieder C (2002). Fast root growth responses, root exudates, and internal detoxification as clues to the mechanisms of aluminium toxicity and resistance: a review. Environ Exp Bot 48: 75-92.
  4. Carretero-Paulet L, Galstyan A, Roig-Villanova I, Martínez-García JF, Bilbao-Castro JR, Robertson DL (2010). Genome-wide classification and evolutionary analysis of the bHLH family of transcription factors in Arabidopsis, poplar, rice, moss, and algae. Plant Physiol 153: 1398-1412.
  5. Castilhos G, Lazzarotto F, Spagnolo-Fonini L, Bodanese-Zanettini MH, Margis-Pinheiro M (2014). Possible roles of basic helix-loop-helix transcription factors in adaptation to drought. Plant Sci 223: 1-7.
  6. Chalker-Scott L (1999). Environmental significance of anthocyanins in plant stress responses. Photochem Photobiol 70: 1-9.
  7. Dinis TC, Madeira VM, Almeida LM (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315: 161-169.
  8. Dong Y, Wang C, Han X, Tang S, Liu S, Xia X, Yin W (2014). A novel bHLH transcription factor PebHLH35 from Populus euphraticaconfers drought tolerance through regulating stomatal development, photosynthesis and growth in Arabidopsis. Biochem Bioph Res Co 450: 453-458.
  9. Fabjan N, Rode J, Kosir IJ, Wang Z, Zhang Z, Kreft I (2003). Tartary buckwheat (Fagopyrum tataricum Gaertn.) as a source of dietary rutin and quercitrin. J Agr Food Chem 51: 6452-6455.
  10. Falcone Ferreyra ML, Rius SP, Casati P (2012). Flavonoids: biosynthesis, biological functions, and biotechnological applications. Front Plant Sci 3: 222. Fini A, Brunetti C, Di Ferdinando M, Ferrini F, Tattini M (2011). Stress-induced flavonoid biosynthesis and the antioxidant machinery of plants. Plant Signal Behav 6: 709-711.
  11. Heim MA, Jakoby M, Werber M, Martin C, Weisshaar B, Bailey PC (2003). The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol