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Journals / Gazi Medical Journal / 2018 / Cilt: 29 - Sayı: 2

Ratlarda Miyokardiyal İskemi-Reperfüzyon Hasarı Sırasında Levosimendan ve Nigella Sativa'nın Eritrosit Deformabilitesine Etkisi

Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats

Levent OKTAR
Metin ALKAN
Ayşegül KÜÇÜK
Faruk METİN COMU
Abdullah ÖZER
Yusuf ÜNAL
Barış MARDİN
Yiğit KILIÇ
Mustafa ARSLAN
Pages
113–115
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results: As a result compared to control group, IR increased the relative resistance, a marker of erythrocyte deformability, significantly (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Ischaemia-reperfusion (IR) injury is a chain of events put in place by tissue ischaemia.
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Key Findings & Data:Myocardial IR was performed by ligating the left anterior descending coronary artery for 30 min, followed by 2 h of reperfusion in the IR (IR), IR-levosimendan (24µg/kg) (IRL) group and IR-nigella sativa (0.2 mL/kg) (IRNS) group.
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Conclusion & Impact:Results: As a result compared to control group, IR increased the relative resistance, a marker of erythrocyte deformability, significantly (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Ischaemia-reperfusion (IR) injury is a chain of events put in place by tissue ischaemia. Reperfusion following the cellular damage causes an active inflammatory response. Erythrocyte deformability and plasma viscosity play a critical role in tissue and organ perfusion. In this study we aimed to evaluate the effect of levosimendan and nigella sativa on erythrocyte deformability during myocardial IR injury in rats. Methods: Twenty-four Wistar albino rats were included in the study. The animals were randomly divided into four experimental groups. The coronary arteries of rats in Group C (control group) were not occluded or reperfused. Myocardial IR was performed by ligating the left anterior descending coronary artery for 30 min, followed by 2 h of reperfusion in the IR (IR), IR-levosimendan (24µg/kg) (IRL) group and IR-nigella sativa (0.2 mL/kg) (IRNS) group. Erythrocyte suspensions formed with a PBS buffer solution containing 5% htc were used to measure the deformability. Results: As a result compared to control group, IR increased the relative resistance, a marker of erythrocyte deformability, significantly (p

Özet

Amaç: İskemi-reperfüzyon (İR) hasarı, doku iskemisi sonucu gerçekleşen bir olaylar zinciridir. Hücresel hasarı takiben reperfüzyon, aktif bir inflamatuar yanıta neden olur. Eritrosit deformabilitesi ve plazma viskozitesi doku ve organ perfüzyonunda kritik bir rol oynar. Bu çalışmada, ratlarda miyokardiyal İR hasarı sırasında levosimendan ve nigella sativa'nın eritrosit deformabilitesi üzerine etkisini araştırmayı amaçladık. Yöntem: Yirmi dört adet Wistar albino rat çalışmaya dahil edildi. Hayvanlar rastgele dört deney grubuna ayrıldı. Grup K'daki (kontrol grubu) ratların koroner arterlerine oklüzyon veya reperfüzyon yapılmadı. Miyokardiyal İR, sol ön inen koroner arterin 30 dakika süreyle bağlanması, ardından İR (İR), İR-levosimendan (24 µg/kg) (İRL) ve İR-nigella sativa (0.2 mL/kg) (İRNS) gruplarında 2 saat reperfüzyon ile gerçekleştirildi. Deformabiliteyi ölçmek için% 5 htc içeren bir PBS tampon çözeltisi ile oluşturulan eritrosit süspansiyonları kullanıldı. Bulgular: Sonuç olarak, kontrol grubuna kıyasla İR, eritrosit deformabilitesinin bir belirteci olan nispi direnci artırdı (p

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Cite This Article

OKTAR, L., ALKAN, M., KÜÇÜK, A., COMU, F. M., ÖZER, A., ÜNAL, Y., MARDİN, B., KILIÇ, Y., ARSLAN, M. (2022). Ratlarda Miyokardiyal İskemi-Reperfüzyon Hasarı Sırasında Levosimendan ve Nigella Sativa'nın Eritrosit Deformabilitesine Etkisi. Gazi Medical Journal, Cilt: 29 - Sayı: 2, 113–115. https://ajindex.com/articles/650784

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References (14)

  1. Robertshaw HJ, Hall GM. Diabetes mellitus: anaesthetic management. Anaesthesia 2006;61:1187-90
  2. McAnulty GR, Robertshaw HJ, Hall GM. Anaesthetic management of patients with diabetes mellitus. Br J Anesth 2000;85:80-90
  3. Peto K, Nemeth N, Brath E, Takacs IE, Baskurt OK, Meiselman HJ, et al. The effects of renal ischemia-reperfusion on hemorheological factors: preventive role of allopurinol. Clin Hemorheol Microcirc 2007;37:347-58
  4. Baskurt OK, Meiselman HJ. Blood rheology and hemodynamics. Semin Thromb Hemostas 2003;29:435-50 5. Brath E, Nemeth N, Kiss F, Sajtos E, Hever T, Matyas L, et al. Changes of local and systemic hemorheological properties in intestinal ischemia-reperfusion injury in the rat model. Microsurgery 2010;30:321-6
  5. Rognoni A, Lupi A, Lazzero M, Bongo AS, Rognoni G. Levosimendan: from basic science to clinical trials. Recent Pat Cardiovasc Drug Discov 2011;6:9-15
  6. Toller WG, Stranz C. Levosimendan, a new inotropic and vasodilatory agent. Anesthesiology 2006;104:556-69
  7. Erdei N, Papp Z, Pollesello P, Edes I, Bagi Z. The levosimendan metabolite OR1986 elicits vasodilation by activating the K(ATP) and BK(Ca) channels in rat isolated arterioles. Br J Pharmacol 2006;148:696-702
  8. Kaheinen P, Pollesello P, Levijoki J, Haikala H. Levosimendan increases diastolic coronary flow in isolated guinea pig heart by opening ATP-sensitive potassium channels. J Cardiovasc Pharmacol 2001;37:367-74
  9. Katircioglu SF, Seren M, Parlar AI, Turan NN, Manavbasi Y, Aydog G, et al. Levosimendan effect on spinal cord ischemia-reperfusion injury following aortic clamping. J Card Surg 2008;23:44-8
  10. Yasa H, Yakut N, Emrecan B, Ergunes K, Ortac R, Karahan N, et al. Protective effects of levosimendan and iloprost on lung injury induced by limb ischemiareperfusion: A rabbit model. J Surg Res 2008;147:138-42
  11. Yakut N, Yasa H, Bahriye Lafci B, Ortac R, Tulukoglu e, Aksun M, et al. The influence of levosimendan and iloprost on renal ischemia-reperfusion: An experimental study. Interact Cardiovasc Thorac Surg 2008;7:235-9
  12. Gali-Muhtasib H, Roessner A, Schneider-Stock R. Thymoquinone: a promising anti-cancer drug from natural sources. Int J Biochem Cell Biol 2006;38:1249–53
  13. El-Dakhakhny M. Studies on the chemical constitution of Egyptian N. sativa L. seeds. Planta Med 1963;11:465–70
  14. Awad AS, Kamel R, Sherief MA. Effect of thymoquinone on hepatorenal dysfunction and alteration of CYP3A1 and spermidine/spermine N-1-acetyltransferase gen