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Journals / Türk Yoğun Bakım Dergisi / 2017 / Cilt: 15 - Sayı: 1

Comparing Effects of Intraventricular Hypertonic Saline and Magnesium Sulfate Application on Diffuse Brain Injury in Rats

Ratlardaki Diffüz Beyin Travmasında Hipertonik Salin ve Magnezyum Sülfatın İntraventriküler Uygulamayla Etkilerinin Karşılaştırılması

Nurcan KIZILCIK
Bayram YILMAZ
Taha KELEŞTEMUR
Ayçiçek ÇEÇEN
Özge KÖNER
Sevgi BİLGEN
Erhan ÇELİKOĞLU
Merih İŞ
Pages
34–38
Publication Date
2022-11-05
DOI
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However, the number of damaged neurons did not showsignificant difference between hypertonic saline and control groups, it was significantly lower inmagnesium group (p

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Objective & Hypothesis:Objective: Diffuse brain injury is one of the most common issues encountered in,patients with trauma and it leads to morbidity and mortality via increased intracranial pressure.The aim of the study was to compare the effects of magnesium sulfate and hypertonic saline ondiffuse brain injury in rats.Materials and Methods: In this study 18 male Sprague-Dawley rats weighing 250-300 g were used.The rats were randomly divided into trauma (control), trauma+magnesium, and trauma+hypertonicsaline groups.
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Key Findings & Data:Asingle dose of 10 µL isotonic saline, magnesium sulphate and hypertonic saline were appliedintraventricularly to the control, magnesium, and hypertonic saline groups, respectively.
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Conclusion & Impact:However, the number of damaged neurons did not showsignificant difference between hypertonic saline and control groups, it was significantly lower inmagnesium group (p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Diffuse brain injury is one of the most common issues encountered in,patients with trauma and it leads to morbidity and mortality via increased intracranial pressure.The aim of the study was to compare the effects of magnesium sulfate and hypertonic saline ondiffuse brain injury in rats.Materials and Methods: In this study 18 male Sprague-Dawley rats weighing 250-300 g were used.The rats were randomly divided into trauma (control), trauma+magnesium, and trauma+hypertonicsaline groups. Traumatic brain injury was induced by modified Feeney head trauma model. Asingle dose of 10 µL isotonic saline, magnesium sulphate and hypertonic saline were appliedintraventricularly to the control, magnesium, and hypertonic saline groups, respectively. Rats weredecapitated 24 hours after the head trauma. Their brains were dissected immediately and storedwith dry ice at -80 °C for histopathological experiments.Results: The number of damaged neurons were significantly higher in both control and hypertonicsaline groups (p=0.001, p=0.008). However, the number of damaged neurons did not showsignificant difference between hypertonic saline and control groups, it was significantly lower inmagnesium group (p

Özet

Amaç: Diffüz beyin hasarı travmada en yaygın karşılaşılan sorunlardan biridir ve artmış kafa içi basıncı vasıtasıyla morbidite ve mortalite yol açar. Çalışmamızın amacı, sıçanlarda magnezyum sülfat ve hipertonik salinin diffüz beyin hasarı üzerine etkisini karşılaştırmaktır. Gereç ve Yöntem: Çalışmamızda 250-300 gr ağırlığında 18 erkek Sprague-Dawley sıçan kullanıldı. Sıçanlar randomize olarak travma (kontrol), travma+magnezyum ve travma+hipertonik salin olmak üzere üç gruba ayrıldı. Travmatik beyin hasarı modifiye Feeney kafa travması modeli ile oluşturuldu. Sırasıyla gruplara 10 mcL izotonik, magnezyum sülfat ve hipertonik salin tek doz, intraventriküler uygulanmıştır. Sıçanlar travmadan 24 saat sonra dekapite edildi. Beyinleri hemen diseke edildi ve histopatolojik deneyler için -80 °C kuru buz ile saklandı. Bulgular: Hasarlı nöron sayısı hem kontrol hem de hipertonik salin grubunda anlamlı olarak yüksekti (p=0,001, p=0,008). Ancak, hipertonik salin ve kontrol grupları arasında anlamlı bir fark yoktu, magnezyum grubunda anlamlı olarak daha düşüktü (p

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Cite This Article

KIZILCIK, N., YILMAZ, B., KELEŞTEMUR, T., ÇEÇEN, A., KÖNER, Ö., BİLGEN, S., ÇELİKOĞLU, E., İŞ, M. (2022). Comparing Effects of Intraventricular Hypertonic Saline and Magnesium Sulfate Application on Diffuse Brain Injury in Rats. Türk Yoğun Bakım Dergisi, Cilt: 15 - Sayı: 1, 34–38. https://ajindex.com/articles/643443

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