Journals / Türk Tarım - Gıda Bilim ve Teknoloji dergisi / 2018 / Cilt: 6 - Sayı: 10

Analysis of Polymorphisms on GH-MspI and IGF1-SnaBI Loci in Five Turkish Native Cattle Breeds

Beş Yerli Türk Sığır Irkında GH-MspI and IGF1-SnaBI Lokuslarındaki Polimorfizmlerin Analizi

Pages
1353–1356
Publication Date
2022-11-04
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

While amonginvestigated population only EAR population was at Hardy-Weinberg equilibrium forIGF1-SnaBI locus, for GH-MspI only, in SAR population no deviation from Hardy-Weinberg equilibrium.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Growth Hormone (GH) and Insulin like Growth Factor-I (IGF1) are members ofsomototrophine axis pathway.
📊
Key Findings & Data:In this study five nativecattle breeds (Native Southern Yellow (NSY), South Anatolian Red (SAR), AnatolianGrey (AG), Native Black (NB), East Anatolian Red (EAR) were investigated by PCRRFLPmethod for GH-MspI and IGF1-SnaBI loci. 198 and 194 samples were analyzedfor GH-MspI and IGF1-SnaBI loci, respectively.
💡
Conclusion & Impact:While amonginvestigated population only EAR population was at Hardy-Weinberg equilibrium forIGF1-SnaBI locus, for GH-MspI only, in SAR population no deviation from Hardy-Weinberg equilibrium.
🔬Design:Research Article
📍Scope:194 samples
⚙️Variables:Domain Parameters

Abstract

Growth Hormone (GH) and Insulin like Growth Factor-I (IGF1) are members ofsomototrophine axis pathway. They play a role in key on several mechanisms such aspostnatal growth, cell differentiation and metabolism. Due to their vital importance,polymorphisms on the genes coding are worth to be understood. In this study five nativecattle breeds (Native Southern Yellow (NSY), South Anatolian Red (SAR), AnatolianGrey (AG), Native Black (NB), East Anatolian Red (EAR) were investigated by PCRRFLPmethod for GH-MspI and IGF1-SnaBI loci. 198 and 194 samples were analyzedfor GH-MspI and IGF1-SnaBI loci, respectively. In both two loci two alleles and threegenotypes were observed. Predominant alleles were A and B for GH-MspI locus IGF1-SnaBI loci, respectively. Frequencies of A and B alleles were calculated between 0.400-0.875 and 0.846-0.903 for GH-MspI locus IGF1-SnaBI loci, respectively. While amonginvestigated population only EAR population was at Hardy-Weinberg equilibrium forIGF1-SnaBI locus, for GH-MspI only, in SAR population no deviation from Hardy-Weinberg equilibrium.

Özet

Büyüme Hormonu (GH) ve İnsülin Benzeri Büyüme Faktörü-I (IGF-I) somatotropin axis yolağının üyeleridirler. Doğum sonrası gelişim, hücre farklılaşması ve metabolizmada gibi mekanizmalarda anahtar rol oynarlar. Hayati önemlerinden dolayı bunları kodlayan genlerin anlaşılmaları da gereklidir. Bu çalışmada beş yerli sığır ırkı (Yerli Güney Sarısı (YGS), Güneydoğu Anadolu Kırmızısı (GAK), Boz Irk (BI), Yerli Kara (YK) ve Doğu Anadolu Kırmızısı (DAK) GH-MspI ve IGF1-SnaBI locusları bakımından PCR-RFLP yöntemi ile incelenmiştir. GH-MspI lokusu için 198, IGF1-SnaBI lokusu için 194 örnek analiz edilmiştir. Her iki lokusta iki allel ve üç genotip gözlenmştir. GH-MspI ve IGF1- SnaBI lokusları için predominant alleler sırasıyla A ve B olarak bulunmuştur. GH-MspI ve IGF1-SnaBI locusları için A ve B allelerinin frekansları sırasıyla 0,400-0,875 ve 0,846-0,903 arasında hesaplanmıştır. İncelenen populasyonlardan sadece DAK IGF1- SnaBI bakımından Hardy-Weinberg dengesindeyken, GH-MspI lokusu için sadece GAK populasyonunda Hardy-Weinberg dengesinden sapma gözlenmiştir.

🔖

Cite This Article

ATA, N., YILMAZ, O., ÖNER, Y., KONCAGÜL, S., ERİŞ, C., ÜNAL, C. (2022). Analysis of Polymorphisms on GH-MspI and IGF1-SnaBI Loci in Five Turkish Native Cattle Breeds. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, Cilt: 6 - Sayı: 10, 1353–1356. https://ajindex.com/articles/643015

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (7)

  1. Akış I, Oztabak K, Gonulalp I, Mengi A, Ün C. 2010. IGF-1 and IGF-1R Gene Polymorphisms in East Anatolian Red and South Anatolian Red Cattle Breeds. Genetika., 46(4):497- 501. Arango J, Echeverri JJ, López A. 2014. Association between a polymorphism in intron 3 of the bovine growth hormone gene and growth traits in Holstein heifers in Antioquia. Genet. Mol. Res., 13: 6191-6199.
  2. Curi RA, Oliveira HND, Silveira AC, Lopes CR. 2005. Effects of polymorphic microsatellites in the regulatory region of IGF1 and GHR on growth and carcass traits in beef cattle. Anim. Genet., 36: 58-62. DOI: 10.1111/j.1365- 2052.2004.01226.x Dybus A. 2002. Associations of growth hormone (GH) and prolactin (PRL) genes polymorphisms with milk production traits in Polish Black-andWhite cattle. Anim. Sci. Pap. Rep., 20: 203-212.
  3. Ge W, Davis ME, Hines HC.1997. Two SSCP alleles detected in the 5’-flanking region of bovine IGF1 gene. Anim. Genet., 28: 155-156.
  4. Gorbani A, Torshizi RV, Bonyadi M, Amirinia C. 2009. Restriction fragment length polymorphism of bovine growth hormone gene intron 3 and its association with testis biometry traits in Iranian Holstein bull. Afr. J. Microbiol. Res., 3 (11): 809-814. Hernández N, Martínez-González JC, Parra-Bracamonte GM, Sifuentes-Rincón AM López-Villalobos N, Morris ST, Briones-Encinia F, Ortega-Rivas E, Pacheco-Contreras VI, Meza-García LA. 2016. Association of polymorphisms in growth hormone and leptin candidate genes with live weight traits of Brahman cattle. Genet. Mol. Res., 15 (3): gmr.15038449 Hoj S, Fredholm M, Larsen NJ, Nielsen VH. 1993. Growth hormone gene polymorphism associated with selection for milk fat production in lines of cattle. Anim. Genet., 24: 91- 96. Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol. Biol. Evol., 33(7): 1870–1874.
  5. Lagziel A, Lipkin E, Ezra E, Soller M, Weller JI: An MspI polymorphism at the bovine growth hormone (bGH) gene is linked to a locus affecting milk protein percentage. Anim Genet, 30 (4): 296-299, 1999.
  6. Lagziel A, Denise S, Hanotte O, Dhara S, Glazko V, Broadhead A, Davoli R, Russo V, Soller M. 2000. Geographic and breed distribution of an MspI PCR-RFLP in the bovine growth hormone (bGH) gene. Anim. Genet., 31:210-213.
  7. Mehmannavaz Y, Amirinia C, Bonyadi M, Torshizi RV. 2010. Association of IGF-1 gene polymorphism with milk production traits and paternal genetic trends in Iranian Holstein bulls. Afr. J. Microbiol. Res., 4(1): 110-114