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Journals / Türk Onkoloji Dergisi / 2019 / Cilt: 34 - Sayı: 4

Mutational Screening of miR-17-92 Cluster and miR-34a by DNA Sequencing in Childhood High-Grade Brain Tumors

Aylar POYRAZ
Avni SARAÇ
Kemali BAYKANER
Ferda ALPASLAN PINARLI
İrem DOĞAN
Aynur OĞUZ
Ceyda KARADENİZ
Abdullah EKMEKÇİ
Arzu OKUR
Faruk GÜÇLÜ PINARLI
Pages
215–222
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further studies are needed to elucidate extremely complicated mechanisms underlying oncogenesis in high-grade central nervous system tumors.

📌 3 Key Takeaways

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Objective & Hypothesis:OBJECTIVEThe miR-17-92 cluster and miR-34a are short non-coding RNAs, which are important in tumorigenesisas they regulate numerous oncogenes and tumor suppressor genes.
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Key Findings & Data:This study aimed to investigate theassociations of mutations/polymorphisms of the miR-17-92 cluster and miR-34a coding sequences withhigh-grade central nervous system malignancies in pediatric patients.METHODSThis study included 53 children with central nervous system malignancies and age- and gender-matched27 healthy volunteers.
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Conclusion & Impact:Further studies are needed to elucidate extremely complicated mechanisms underlying oncogenesis in high-grade central nervous system tumors.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVEThe miR-17-92 cluster and miR-34a are short non-coding RNAs, which are important in tumorigenesisas they regulate numerous oncogenes and tumor suppressor genes. This study aimed to investigate theassociations of mutations/polymorphisms of the miR-17-92 cluster and miR-34a coding sequences withhigh-grade central nervous system malignancies in pediatric patients.METHODSThis study included 53 children with central nervous system malignancies and age- and gender-matched27 healthy volunteers. Genomic DNAs were extracted from the paraffin-embedded tumor tissues (n=53)and peripheral blood samples (n=15) in the patient group and from the peripheral blood samples in thecontrol group and were analyzed for mutations/polymorphisms of the miR-17-92 cluster and miR-34acoding sequences by DNA sequencing method.RESULTSThere were no copy number alterations, amplifications, deletions, insertions, duplications, rearrangements, single nucleotide polymorphisms or mutations in the miR-17-92 cluster and miR-34a coding sequences of tumor tissue or blood samples in the patient group and of blood samples in the control group.CONCLUSIONIn children with high-grade brain tumors, no mutation was detected, leading to failures in regulations ofmiRNA coding DNA sequences of miR-17-92 and miR-34a. Further studies are needed to elucidate extremely complicated mechanisms underlying oncogenesis in high-grade central nervous system tumors.

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Cite This Article

POYRAZ, A., SARAÇ, A., BAYKANER, K., PINARLI, F. A., DOĞAN, İ., OĞUZ, A., KARADENİZ, C., EKMEKÇİ, A., OKUR, A., PINARLI, F. G. (2022). Mutational Screening of miR-17-92 Cluster and miR-34a by DNA Sequencing in Childhood High-Grade Brain Tumors. Türk Onkoloji Dergisi, Cilt: 34 - Sayı: 4, 215–222. https://ajindex.com/articles/639214

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