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Journals / Türk Onkoloji Dergisi / 2020 / Cilt: 35 - Sayı: 3

Assessment of the Therapeutic Potential of Epigallocatechin Gallate and/or Metformin on Oral Squamous Cell Carcinoma

Nermeen ABUBAKR
Dina SABRY
Eman AHMED
Walaa IBRAHIM
Nagla ADLI
Amany NASR
Nehal Abd-El HAMEED
Pages
289–297
Publication Date
2022-11-06
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Reactive oxygen species (ROS) and vascular endothelialgrowth factor were assessed by ELISA.RESULTSResults showed that cells treated with metformin showed a notable rise in caspase 3 and a significantdecrease in all parameters.

📌 3 Key Takeaways

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Objective & Hypothesis:OBJECTIVESquamous cell carcinoma is a prevalent tumor in the head and neck region.
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Key Findings & Data:Current treatments havelow survival rates.
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Conclusion & Impact:Reactive oxygen species (ROS) and vascular endothelialgrowth factor were assessed by ELISA.RESULTSResults showed that cells treated with metformin showed a notable rise in caspase 3 and a significantdecrease in all parameters.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVESquamous cell carcinoma is a prevalent tumor in the head and neck region. Current treatments havelow survival rates. Therefore, physicians are seeking alternative therapies. This work compared the effectsof Epigallocatechin-3-gallate (EGCG), metformin and a combination of both on oral squamouscell carcinoma.METHODSThis study was performed on human epithelial type 2 (Hep2) cells. Cell viability was estimated by MTTassay. Gene expression of platelet-derived growth factor, interleukin 6, caspase 3 and survivin was evaluatedby a real-time polymerase chain reaction. Reactive oxygen species (ROS) and vascular endothelialgrowth factor were assessed by ELISA.RESULTSResults showed that cells treated with metformin showed a notable rise in caspase 3 and a significantdecrease in all parameters. EGCG had an insignificant change in all parameters tested except a significantdecrease in ROS. A combination of both showed a remarkable elevation in caspase 3 and a morepronounced decrease in all parameters.CONCLUSIONIt was concluded that EGCG exhibited a synergistic anti-proliferative, anti-angiogenic, pro-apoptoticand antioxidant activity on the Hep2 cell line when combined with metformin.

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Cite This Article

ABUBAKR, N., SABRY, D., AHMED, E., IBRAHIM, W., ADLI, N., NASR, A., HAMEED, N. A. (2022). Assessment of the Therapeutic Potential of Epigallocatechin Gallate and/or Metformin on Oral Squamous Cell Carcinoma. Türk Onkoloji Dergisi, Cilt: 35 - Sayı: 3, 289–297. https://ajindex.com/articles/639165

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