SavedAdd your journal

Journals / Türkiye Parazitoloji Dergisi / 2002 / Cilt: 26 - Sayı: 2

Investigation of the validity of Western blotting for the serological diagnosis of fasciolasis

Fasciolasisin serolojik tanısında Western blotting yönteminin geçerliliğinin araştırılması

M. Ziya ALKAN
Nilüfer DAYANGAÇ
Aylin BABAOĞLU
Nermin ŞAKRU
Metin KORKMAZ
Derya DİRİM
Pages
161–165
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

There was specificity to at least one of the low molecular weight fractions of 5-12, 17, 25-29, 32, 40-43, 58 kDa, showing the efficiency of Western blotting as a confirmation test, especially in samples found to be positive by other serological tests.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the sera of patients, who had been parasitologically or radiologically diagnosed as early or late stages of fasciolasis, were examined by Western blotting.
📊
Key Findings & Data:The results were evaluated by determining the polypeptide fractions, reacting to serum antibodies.
💡
Conclusion & Impact:There was specificity to at least one of the low molecular weight fractions of 5-12, 17, 25-29, 32, 40-43, 58 kDa, showing the efficiency of Western blotting as a confirmation test, especially in samples found to be positive by other serological tests.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, the sera of patients, who had been parasitologically or radiologically diagnosed as early or late stages of fasciolasis, were examined by Western blotting. The results were evaluated by determining the polypeptide fractions, reacting to serum antibodies. There was specificity to at least one of the low molecular weight fractions of 5-12, 17, 25-29, 32, 40-43, 58 kDa, showing the efficiency of Western blotting as a confirmation test, especially in samples found to be positive by other serological tests.

Özet

Bu çalışmada, parazitolojik ve radyolojik olarak erken ve geç dönem fasciolasis tanısı konmuş hastalara ait serum örnekleri. Western blotting (WB) yöntemi ile incelenmiş ve serum örnekleri tarafından tanınan polipeptid fraksiyonları değerlendirilmiştir. Sonuçlar, hasta serum antikorlarının özellikle düşük moleküler ağırlıklı fraksiyonlardan 5-12, 17, 25-29, 32, 40-43, 58 kDa'luk antijenlerden en az birine yüksek spesilîte gösterdiğini, diğer serolojik yöntemlerle elde edilecek sonuçların doğrulanmasında, WB yönteminin etkin ve güvenli bir test olduğunu ortaya koymuştur.

🔖

Cite This Article

ALKAN, M. Z., DAYANGAÇ, N., BABAOĞLU, A., ŞAKRU, N., KORKMAZ, M., DİRİM, D. (2022). Investigation of the validity of Western blotting for the serological diagnosis of fasciolasis. Türkiye Parazitoloji Dergisi, Cilt: 26 - Sayı: 2, 161–165. https://ajindex.com/articles/630975

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Altıntaş K, Kurt N, Kıyan M, 1991. Fasciola hepatica'ya. bağlı bir kolesistit olgusu. T Parazitol Derg, 15(1): 39-42
  2. Arafa MS, Abaza SM, El-Shewy KA, Mohareb EW, El- Moamly AA, 1999. Detection of Fasciola- specific excretory/secretory (E/S) protein fraction bant (49.5 kDa) and its utilization in diagnosis of early fascioliasis using different diagnostic techniques. J Egypt Soc Parasitol, 29(3): 911 -926 3. Atalay F, Kırımlıoğlu V, Dağlı Ü, Akıncıoğlu T, Akaoğlu M, Seven C, 1993. Human fascioliasis. Surgery Today, 23: 366-369
  3. Boyacıoğlu S, Dalay R, Hilmioğlu F, Akoğlu M, Şahin M, 1991. The Cholangiographic Findings of Human Fascioliasis: A report Of Two Cases. Gastroenteroloji, 2 (2): 130-132
  4. Büyükbaba Ö, Özkan E, Büyükuncu Y, Büğet E, 1996. Fasciola hepatica'ya bağlı bir kolesistit olgusu. Klimik Derg, 9(2): 98-99
  5. Demir A, Cümşüdov C, Topgül K, Baydar B, Hasıroğlu F, Taner Ş, Şahin HC, 1996. Koledokta Fasciola hepatica. Gastroenteroloji, 7(l-ek): 90 7. Demirci M. 1999. İsparta yöresinde Eosinofıli saptanan hastalarda fascioliosis. Ege Üniversitesi Sağlık Bilimleri Enstitüsü, Doktora Tezi
  6. Diaz A, Li-elias O, Knoll O, Garcias C, Espino AM, 1998. Identification of the immunogenecity of Fasciola hepatica by Western Blot, recognized by sera of experimentally infected rats, (ingilizce özet). Cuban Rev Med Trop, 50(l):12-7
  7. Doğan N, Akgün Y, 1998. Eskişehir yetiştirme yurdunda barsak parazitleri prevalansı. TParazitol Derg, 22(3): 282-286
  8. Ersoy N, Tınar R, 1992. Bursa sosyal sigortalar hastanesinde bir distomatoz (Fasciola hepatica ) olgusu. T Parazitol Derg, 16(3-4): 73-79
  9. Espino AM, Dumenigo BE, Fernandez R, Finlay CM, 1987. Immunodiagnosis of human fascioliasis by Enzyme-linked immunosorbent assay using excretory-secretory products. Am J Trop Med Hyg, 37(3): 605-608
  10. Güçlü F, Dik B, Ağaoğlu M, 1995. Bir kadında Fasciola hepatica''dan ileri gelen fascioliasis olgusu. T Parazitol Derg,.19(3): 370-374
  11. Hammami H, Ayadi A, Camus D, Dutoit E, 1997. Diagnostic value of the demonstration os specific antigens of Fasciola hepatica by Western Blot technique (İngilizce özet). Parasit,4(3): 291-5
  12. Han JK, Han D, Choi BI, Han MC, 1996. MR findings in human fascioliasis. Trop Med Int Health, 1(3): 367-372
  13. Hillyer GV, Galanes MS, Rodriguez-Perez J, Bjorland J, Lagrava MS, Guzman SR, Bryan RT, 1992. Use of FAST- ELISA and EITB to determine the prevalence of human fascioliasis in the Bolivian Altiplano. Am J Trop Med Hyg,46(