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Journals / Türkiye Parazitoloji Dergisi / 2005 / Cilt: 29 - Sayı: 2

Comparison of microscopy and EIA in the diagnosis of giardia intestinalis in stool specimens

Giardiasisin tanısında enzym immun assay (EIA) ve direkt inceleme yöntemlerinin karşılaştırılması

Tuncer ÖZEKİNCİ
Adnan SUAY
Saffet ELÇİ
Aslıhan UZUN
Nezahat AKPOAT
Selahattin ATMACA
Pages
89–92
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

When the patient and control groups were compared, a significant difference was observed between the two methods (p

📌 3 Key Takeaways

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Objective & Hypothesis:In this study; we compared the direct microscopic method and EIA test in the investigation of the stools of patients with gastrointestinal symptoms who presented at clinics.
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Key Findings & Data:A total of 188 stool specimens collected from clinics were investigated by direct microscopy using native-Lugol preparations.
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Conclusion & Impact:When the patient and control groups were compared, a significant difference was observed between the two methods (p
🔬Design:Research Article
📍Scope:141 specimens
⚙️Variables:Domain Parameters

Abstract

In this study; we compared the direct microscopic method and EIA test in the investigation of the stools of patients with gastrointestinal symptoms who presented at clinics. A total of 188 stool specimens collected from clinics were investigated by direct microscopy using native-Lugol preparations. Giardia cysts and/or trophozoites were observed in 141 specimens. There were no Giardia cysts and/or trophozoites or any other intestinal parasites detected in the other 47 stool specimens. The RIDASCREENR EIA kit procedure was applied in all specimens. Out of 141 specimens positive with direct microscopy, 136 specimens were positive with the EIA test and 5 specimens, negative. Parasites were not found in 47 stool specimens with direct microscopy. Of these, 38 specimens were negative with the EIA test and 9 specimens, positive. When the patient and control groups were compared, a significant difference was observed between the two methods (p

Özet

Bu çalışmada, çeşitli gastrointestinal şikayetlerle hastanemiz polikliniklerine başvuru yapan hastaların dışkı örneklerinin incelenmesinde, rutin mikroskobik yöntem ve EIA testi karşılaştırılmıştır. Toplanan 188 dışkı örneğinde, nativ-lugol ile direkt mikroskobik inceleme yapılmış, 141 örnekte Giardia intestinalis kist ve/veya trofozoiti bulunurken, 47 dışkı örneğinde herhangi bir parazit tespit edilememiştir. Tüm örneklere, RIDASCREEN EIA kit prosedürü uygulanmıştır. Direkt mikroskobisi pozitif bulunan 141 örneğin 136 'sı EIA testi ile pozitif; 5'i negatif olarak saptanmıştır. Direkt mikroskobide parazit saptanmayan 47 dışkı örneğinin 38'i EIA ile negatif; 9'u pozitif olarak belirlenmiş, iki yöntem hasta ve kontrol grupları göz önüne alınarak karşılaştırıldığında istatiksel olarak anlamlı fark bulunmuştur (p

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Cite This Article

ÖZEKİNCİ, T., SUAY, A., ELÇİ, S., UZUN, A., AKPOAT, N., ATMACA, S. (2022). Comparison of microscopy and EIA in the diagnosis of giardia intestinalis in stool specimens. Türkiye Parazitoloji Dergisi, Cilt: 29 - Sayı: 2, 89–92. https://ajindex.com/articles/630867

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