Journals / Türkiye Parazitoloji Dergisi / 2005 / Cilt: 29 - Sayı: 3

Molecular genetic characterization of genus echinococcus

Echinococcus cinsinin moleküler genetik karakterizasyonu

Pages
171–176
Publication Date
2022-11-04
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this review, various data concerning molecular typing methods of the genus Echinococcus have been summarized.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Morphological, biological and epidemiological features of the adult and metacestode stage of the genus Echinococcus have been the most frequently used criteria for the characterization of species.
📊
Key Findings & Data:But strain characterization is more complex and is based on the morphological, biological, biochemical, epidemiological and molecular features.
💡
Conclusion & Impact:In this review, various data concerning molecular typing methods of the genus Echinococcus have been summarized.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Morphological, biological and epidemiological features of the adult and metacestode stage of the genus Echinococcus have been the most frequently used criteria for the characterization of species. But strain characterization is more complex and is based on the morphological, biological, biochemical, epidemiological and molecular features. Although morphological and biological studies have provided extremely useful information for strain characterization, that these features may be variable must be taken into consideration. They may be influenced by host and environmental factors and may not reflect distinctness at a genetic level. Although, conventional techniques may be affected by the environment or host, molecular techniques allow a direct characterization of the genome of the parasite and they are not affected by environmental factors. In this review, various data concerning molecular typing methods of the genus Echinococcus have been summarized.

Özet

Echinococcus cinsinin, erişkin ve metacestod safhalarının; morfolojik, biyolojik ve epidemiyolojik özellikleri tür karakterizasyonunda en çok kullanılan kriterlerdir. Fakat suş tayini daha karmaşık olup morfolojik, biyolojik, biyokimyasal, epidemiyolojik ve moleküler kriterlere bağlıdır. Morfolojik ve biyolojik çalışmalar suş tayininde çok faydalı bilgiler sağlamasına rağ¬men, bu özelliklerin değişken olabileceği göz önüflde bulundurulmalıdır. Bunlar konak ve çevresel faktörlerden etkilenebilir ve genetik düzeydeki farklılığı yansıtmayabilirler. Klasik teknikler çevreden ve konaktan etkilenmesine rağmen, moleküler teknikler parazit genomunun direk karakterizasyonuna olanak sağlar ve çevresel faktörlerden etkilenmezler. Bu derlemede Echinococcus cinsinin tiplendirilmesinde kullanılan moleküler teknikler özetlenmiştir.

🔖

Cite This Article

ÜTÜK, A. E., KÖROĞLU, E., ŞİMŞEK, S. (2022). Molecular genetic characterization of genus echinococcus. Türkiye Parazitoloji Dergisi, Cilt: 29 - Sayı: 3, 171–176. https://ajindex.com/articles/630857

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Amman RW, Eckcrt J, 1995. Clinical diagnosis and treatment of echinococcosis in humans. Thompson RCA. Lymbery AJ Eds. Echinococcus and Hydatid Disease. Wallingford, Oxon:CAB International, p. 411-463
  2. Anon. RAPDs. İnternet erişim adresi. http://www.usask.ca/agriculture/plantsci/classes/plsc416/projects _2002/pawlin/resources 3. Anon. SSCP (Single Stranded Conformational Polymorphism). Internet erişim adresi. http//www.egn.wageningenur.nl/pgr/research/molgen/sses.htm 4. Arda M, 1994. Biyoteknoloji (Bazı Temel İlkeler). İkinci baskı. Ankara: Kükem Derneği Bilimsel Yayınlan. No:2 5. Bowles J, Blair D, McManus DP, 1992. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol., 54: 165-173
  3. Bowles J, Blair D, McManus DP, 1994. Molecular genetic characterization of the cervid strain (" northern form") of Echinococcus granulosus. Parasitology, 109(2): 215-221
  4. Bowles J, McManus DP, 1993. Molecular variation in Echinococcus. Açta Trop., 53: 291-305
  5. Bowles J, McManus DP, 1993. Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-. based RFLP method. Mol Biochem Parasitol., 57: 231-239
  6. Bowles J, McManus DP, 1993. NADH dehydrogenase I gene sequences compared for species and strains of the genus Echinococcus. hit J Parasitol.,23: 969-972
  7. Cotton RGH, 1997. Mutation Detection. Oxford: Oxford University Pres
  8. Eckert J, Thompson RCA, 1997. Intraspesific variation of Echinococcus granulosus and- related species with emphises on their infecdvity to humans. Acta Tropica, 64: 19-34
  9. Ellsworth DL, Rittenhouse KD, Honeycutt RL 3. Artifactual variation in randomly amplified polymorphic UNA banding patterns. Bio Techniques, 14(2): 214-217
  10. Gasser RB, 1997. Mutation scaning methods for the analysis of parasite genes, bit J Parasitol., 27: 1449-1463
  11. Gasser RB, 1999. PCR-based technology in veterinary parasitology. Vet Parasitol., 84: 229-258
  12. Gasser RB, Monti JR, 1997. Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA. Mol Cell Prob 11,201-209
  13. Gasser RB, Zhu X, McManus DP, 1998. Dideoxy fingerprinting: application to the genotyping of Echinococcus.Int J Parasitol, 28: 1775-1779
  14. Gasser RB, Zhu X, McManus DP, 1998. Display of sequence variation in PCR-amplified mitochondrial DNA regions of Echinococcus by single-strand conformation polymorphism. Acta Tropica, 71: 107-115
  15. Gonzalez LM, Daniel-Mwambete K, Montero E,