Journals / Türkiye Parazitoloji Dergisi / 2010 / Cilt: 34 - Sayı: 4

Diagnosis of protozoa by loop-mediated isothermal amplification: (LAMP)

Protozoonların tanısında ilmiğe dayalı izotermal çoğaltma yöntemi (LAMP)

Pages
207–211
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The aim of this collation is to give information about the usage of the technique of LAMP in the identification of the protozoa which are important for human health and the comparison between the technique of LAMP and other molecular methods

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Plasmodium, Isospora, Toxoplasma ,Babesia and Cryptosporidium are parasites which have a significant role in human health.
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Key Findings & Data:The location in which the protozoon settles in the body, the way that it leaves the host, and the sample which is send from the clinic are the elements of the general methods used in the diagnosis of the ailments caused from protozoan.
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Conclusion & Impact:The aim of this collation is to give information about the usage of the technique of LAMP in the identification of the protozoa which are important for human health and the comparison between the technique of LAMP and other molecular methods
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Plasmodium, Isospora, Toxoplasma ,Babesia and Cryptosporidium are parasites which have a significant role in human health. The location in which the protozoon settles in the body, the way that it leaves the host, and the sample which is send from the clinic are the elements of the general methods used in the diagnosis of the ailments caused from protozoan. Classical methods are not adequate for the identification of some protozoon; also, molecular methods have to be used in designating the distinctions between the species. In recent years, one of the molecular methods which has been used frequently in the identification of protozoon is the technique of LAMP. With the aid of the LAMP technique, from which it is possible to obtain reliable outcomes without the contribution of technical skills and professional equipment, in constant temperature it is possible to have a great number of copies from the targeted DNA in a short period. The aim of this collation is to give information about the usage of the technique of LAMP in the identification of the protozoa which are important for human health and the comparison between the technique of LAMP and other molecular methods

Özet

Protozoanlardan Apicomplexan şubesinin insan sağlığı yönünden çok önemli parazit cinsleri Plasmodium, Isospora, Toxoplasma, Babesia ve Cryptosporidium'dur. Protozoer hastalıkların tanısında kullanılan genel yöntemler, protozoonun vücutta yerleştiği yere, konağı terk etme yoluna ve klinikten gönderilen örneğe dayanır. Bazı protozoanların tanımlanmasında tek başına klasik yöntemler yeterli olmayıp türler arasındaki farklılığı belirlemek için moleküler metotlar kullanılmak zorundadır. Son yıllarda protozoanların tanımlanmasında sıklıkla kullanılan moleküler yöntemlerden biri de LAMP tekniğidir. Çok fazla teknik beceri ve profesyonel ekipmanlara gerek olmayan ancak güvenilir sonuçların elde edildiği lamp tekniğiyle sabit sıcaklıkta hedef DNA'dan kısa bir sürede çok fazla sayıda kopya elde etmek mümkündür. Bu çalışmanın amacı insan sağlığı yönünden önemli olan protozoanların tanımlanmasında lamp tekniğinin kullanılması ve diğer moleküler tekniklerle kıyaslanması hakkında bilgi vermektir.

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Cite This Article

KOLÖREN, Z., AVŞAR, C., ŞEKEROĞLU, Z. A. (2022). Diagnosis of protozoa by loop-mediated isothermal amplification: (LAMP). Türkiye Parazitoloji Dergisi, Cilt: 34 - Sayı: 4, 207–211. https://ajindex.com/articles/630533

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References (13)

  1. Notomi T, Okayama H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop- mediated isothermal amplification of DNA. Nucleic Acids Res, 2000; 28: 63
  2. Nagamine K, Hase T, Notomi T. Accelerated reaction by Loopmediated isothermal amplification using loop primers. Mol Cell Probes, 2002; 16: 223-9
  3. Paris DH, Blacksell SD, Newton PN, Day NP. Simple, rapid and sensitive detection of Orientia tsutsugamushi by loop-isothermal DNA amplification. Trans. R. Soc. Trop. Med. Hyg. 2008; 102: 1239-46
  4. Karanis P. European symposium on waterborne pathogens in surface water, groundwater and drinking water. April, 2007 in Luxemburg
  5. Zhang H, Thekisoe OMM, Aboge GO, Kyan H, Yamagishi J, Inoue J, et al. Sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method of Toxoplasma gondii. Experimental Parasitology, 2009; 122: 47-50
  6. Mori Y, Hirano T, Notomi T. Sequence specific visual detection of LAMP reactions by addition of cationic polymers. BMC Biotechnol, 2006; 6: 3
  7. Soliman H, El-Matbouli M. An inexpensive and rapid diagnostic method of the koi herpesvirus (KHV) infection by loop-mediated isothermal amplification. Virol J, 2005; 2: 83
  8. Mohammed A, Bakheit DT, Lily AP, Thekisoe OMM, Mbati PA, Ongerth Jerry, et al. Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing. Veterinary parasitology, 2008; 158: 11-22
  9. Karanis P, Thekisoe O, Kiouptsi K, Ongerth J, Igarashi I, Inoue N. Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples. Appl. Environ. Microbiol. 2007; 73: 5660-2
  10. Liu A, Yin H, Guan GQ, Schnittger L, Liu ZJ, Ma ML, et al. At least two genetically distinct large Babesia species infective to sheep and goats in China. Veterinary Parasitology, 2007; 147: 246-51
  11. Guan G, Chauvin A, Luo J, Inoue N, Moreau E, Liu Z, et al. The development and evaluation of a loop-mediated isothermal amplification (LAMP) method for detection of Babesia spp. infective to sheep and goats in China. Experimental Parasitology, 2006; 120: 39-44
  12. Sotiriadou I, Kartashev V, Kourenti C, Tsvetkova N, Stojanova K. Investigations on Giardia and Cryptosporidium in drinking water supplies of Rostov region (Southern Russia) and Sofia (Bulgaria). Environ Res. 2006; 102: 475-81
  13. Alhassan