Detection of Anaplasma / Ehrlichia Species of Cattle and Ticks in Aydın Region

Objective & Hypothesis:Objective: The aim of this study is to detect the Anaplasma/Ehrlichia species of cattle and ticks and to provide knowledge on the prevalence of these species during sampling periods.Methods: A total of 679 blood and 186 tick samples were collected from the Osmanbükü, Akçaova, Dalama, and Söke districts of Aydın.

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Key Findings & Data:Mixed infections were detected in 50 blood samples.

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Conclusion & Impact:The analysis indicated that collected ticks were infected with different Anaplasma/Ehrlichia species

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study is to detect the Anaplasma/Ehrlichia species of cattle and ticks and to provide knowledge on the prevalence of these species during sampling periods.Methods: A total of 679 blood and 186 tick samples were collected from the Osmanbükü, Akçaova, Dalama, and Söke districts of Aydın. The samples were screened with genus polymerase chain reaction (PCR) for Anaplasma/Ehrlichia spp., species-specific polymerase chain reaction for Anaplasma marginale and A. centrale, and nested PCR for A. bovis and A. phagocytophilum.Results: A. centrale was detected in Söke during September and in Dalama and Akçaova during March, June, September, and December. A. marginale was detected in Osmanbükü during June; in Söke during March and December; in Akçaova during June, September, and March; and in Dalama during the entire sampling period. A. phagocytophilum was detected in all regions during the entire sampling period. None of the samples were positive for A. bovis. Mixed infections were detected in 50 blood samples. A. marginale and A. phagocytophilum were detected in the tick samples.Conclusion: In this study, A. phagocytophilum was abundantly detected compared with A. marginale and A. centrale. A. phagocytophilum and A. centrale were extensively found in Akçaova and A. marginale was mostly seen in Dalama. Parasites were extensively detected in September and March. The analysis indicated that collected ticks were infected with different Anaplasma/Ehrlichia species

Özet

Amaç: Bu çalışmada, sığır ve kenelerde Anaplasma/Ehrlichia türlerinin belirlenmesi ve bu türlerin örnekleme dönemlerindeki yaygınlığı hakkında bilgi edinilmesi amaçlanmıştır. Yöntem: Aydın'a bağlı Osmanbükü, Akçaova, Dalama ve Söke'den sığırlardan toplam 679 kan ve 186 kene örneği toplanmıştır. Toplanan örneklerde Anaplasma/Ehrlichia türlerini soy düzeyinde tespit eden polimeraz zincir reaksiyonu (PZR) yapılmış, ayrıca A. marginale ile A. centrale türleri, tür spesifik PZR ve A. bovis ile A. phagocytophilum türleri ise nested PZR ile belirlenmiştir. Bulgular: Söke'de sadece Eylül, Dalama ve Akçaova'da ise Mart, Haziran, Eylül ve Aralık'ta A. centrale tespit edilmiştir. A. marginale Osmanbükü'nde sadece Haziran'da, Söke'de Aralık ve Mart, Akçaova'da Haziran, Eylül ve Mart, Dalama'da ise tüm örnekleme dönemlerinde belirlenmiştir. A. phagocytophilum ise tüm bölgelerde örnekleme yapılan bütün dönemlerde tespit edilmiştir. İncelenen örneklerde A. bovis'e rastlanmamıştır. Toplam 50 kan örneğinde miks enfeksiyona rastlanmıştır. Kene havuzlarında A. marginale ve A. phagocytophilum tespit edilmiştir.Sonuç: Bu çalışmada, A. phagocytophilum türünün A. marginale ve A. centrale'ye oranla daha yüksek olarak bulunduğu belirlenmiştir. A. phagocytophilum ile A. centrale en yoğun olarak Akçaova'da tespit edilirken, A. marginale Dalama'da en yoğun olarak tespit edilmiştir. Etkenler en yoğun Eylül ve Mart'ta tespit edilmiştir. Analizler kenelerin farklı Anaplasma/Ehrlichia türleri ile enfekte olduğunu göstermiştir

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Cite This Article

BAKIRCI, S., BİLGİÇ, H. B., ÜNLÜ, A. H., HOŞGÖR, M., EREN, H., KARAGENÇ, T. (2022). Detection of Anaplasma / Ehrlichia Species of Cattle and Ticks in Aydın Region. Türkiye Parazitoloji Dergisi, Cilt: 39 - Sayı: 4, 291–298. https://ajindex.com/articles/630193

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