Journals / Türkiye Parazitoloji Dergisi / 2018 / Cilt: 42 - Sayı: 4

In-vitro Anthelmintic Effects of Aqueous and Ethanolic Extracts of Marrubium vulgare Leaves Against Bovine Digestive Strongyles

Marrubium vulgare (karaderme) Yapraklarının Sulu ve Etanolik Ekstraktlarının Sığır Sindirim Strongilozuna Karşı in-vitro Antelmintik Etkileri

Pages
262–267
Publication Date
2022-11-05
DOI
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The larval mortality rate of both AE and EE from M. vulgare showed that the extracts at 50 mg/ml exhibited 45.8±1.99%and 51±2.53%, respectively, at 24h.Conclusion: The findings of the present study showed that AE and EE of M. vulgare leaves have a potential anthelmintic activity on eggs and larvaeof bovine strongyles parasites in vitro.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of the present study was to evaluate in vitro the anthelmintic activity of Marrubium vulgare L. growing in Algeria against digestivestrongyles in naturally infected bovine.Methods: The anthelmintic activities of the extracts were evaluated using the egg hatch assay and larval mortality assay.
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Key Findings & Data:Ethanolic (EE) and aqueous extracts (AE) were tested at 0.78, 1.55, 3.1, 6.2, 12.5, 25, and 50 mg/ml.
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Conclusion & Impact:The larval mortality rate of both AE and EE from M. vulgare showed that the extracts at 50 mg/ml exhibited 45.8±1.99%and 51±2.53%, respectively, at 24h.Conclusion: The findings of the present study showed that AE and EE of M. vulgare leaves have a potential anthelmintic activity on eggs and larvaeof bovine strongyles parasites in vitro.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of the present study was to evaluate in vitro the anthelmintic activity of Marrubium vulgare L. growing in Algeria against digestivestrongyles in naturally infected bovine.Methods: The anthelmintic activities of the extracts were evaluated using the egg hatch assay and larval mortality assay. Leaves powder of M. vulgareas extracted by maceration. Ethanolic (EE) and aqueous extracts (AE) were tested at 0.78, 1.55, 3.1, 6.2, 12.5, 25, and 50 mg/ml. Albendazole anddimethyl sulfoxide were used as positive and negative controls at concentrations20 mg/ml and 3%, respectively.Results: The mean embryonation rate was maximum in AE and EE (48.4±3.47% and 54.2±2.87%, respectively) of M. vulgare leaves. The extracts of M.vulgare leaves high effects were observed with 50 mg/ml, but the lowest reduction on parasite eggs hatchability was observed in cultures exposed to0.78 mg/ml to both extracts. The larval mortality rate of both AE and EE from M. vulgare showed that the extracts at 50 mg/ml exhibited 45.8±1.99%and 51±2.53%, respectively, at 24h.Conclusion: The findings of the present study showed that AE and EE of M. vulgare leaves have a potential anthelmintic activity on eggs and larvaeof bovine strongyles parasites in vitro.

Özet

Amaç: Bu çalışmanın amacı Cezayir’de yetişen Marrubium vulgare yapraklarının, doğal yollarla enfekte olmuş sığırlarda sindirim strongilozuna karşı in vitro antelmintik etkisini değerlendirmektir. Yöntemler: Ekstraktların antelmintik aktiviteleri yumurtadan çıkma testi (egg hatch assay) ve larva mortalite testi (larval mortality assay) kullanılarak değerlendirildi. M. vulgare yaprak tozları maserasyon ile ekstrakte edildi. 0.78, 1.55, 3.1, 6.2, 12.5, 25 ve 50 mg/mL ölçülerinde etanolik (EE) ve sulu ekstraktlar (AE) test edildi. Sırasıyla 20 mg/mL ve 3% konsantrasyonlarında albendazol and dimetil sülfoksit, pozitif ve negatif kontroller olarak kullanıldılar. Bulgular: M. vulgare yapraklarının ortalama embriyonlaşma oranı AE ve EE’de maksimum idi (sırasıyla %48,4±3,47 ve %54,2±2,87). M. vulgare ekstraktlarının yapraklarının 50 mg/mL ile yüksek etki gösterdiği gözlenmiştir, ancak her iki ekstrakta da, 0,78 mg/mL’ye maruz bırakılan kültürlerde en düşük parazit yumurta kuluçka randımanı gözlenmiştir. M. vulgare’den elde edilen AE ve EE’nin larva mortalite oranları, 24 saatte 50 mg/mL’deki ekstraktlar için sırasıyla %45,8±1,99 ve %51±2,53 olarak gözlenmiştir. Sonuç: Bu çalışmanın bulguları, M. vulgare yapraklarının AE ve EE’sinin, in vitro olarak sığır strongil parazitlerinin yumurtaları ve larvaları üzerinde potansiyel bir antelmintik etkiye sahip olduğunu göstermiştir.

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Cite This Article

MOUSSOUNI, L., BENHANIFA, M., AYAD, A. (2022). In-vitro Anthelmintic Effects of Aqueous and Ethanolic Extracts of Marrubium vulgare Leaves Against Bovine Digestive Strongyles. Türkiye Parazitoloji Dergisi, Cilt: 42 - Sayı: 4, 262–267. https://ajindex.com/articles/630054

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