Journals / Türkiye Parazitoloji Dergisi / 2019 / Cilt: 43 - Sayı: 1

Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016

Ankara Yöresindeki Kedilerde 2016 Yılında Sabin- Feldman Dye Testi (SFDT) ile Anti-Toxoplasma gondii Antikorlarının Araştırılması

Pages
5–9
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Comparison of demographic data with SFDT results showed that positive test results did not differ according to gender and age (P=0.803 and P=0.991, respectively).

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Objective & Hypothesis:Objective: Toxoplasmosis, in which obligate intracellular protozoa Toxoplasma gondii (T.gondii) is the causative organism, is a multisystemic disease that can be seen all over the world and can impair all vertebrates.
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Key Findings & Data:Material of the study was obtained by taking blood samples from cats who were admitted to the clinics between March 2016 and October 2016 in Ankara.
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Conclusion & Impact:Comparison of demographic data with SFDT results showed that positive test results did not differ according to gender and age (P=0.803 and P=0.991, respectively).
🔬Design:Research Article
📍Scope:2016 Verileri
⚙️Variables:Domain Parameters

Abstract

Objective: Toxoplasmosis, in which obligate intracellular protozoa Toxoplasma gondii (T.gondii) is the causative organism, is a multisystemic disease that can be seen all over the world and can impair all vertebrates. The only hosts known for T.gondii are members of Felidae family. Our study aimed to determine anti-Toxoplasma gondii antibodies with Sabin-Feldman Dye Test (SFDT) in cats in Ankara. It’s aimed to evaluate the current situation in terms of Toxoplasmosis spread by comparing our findings with previous studies in the same region. Methods: Rh strain of Toxoplasma used in our study is maintained in our laboratory. SFDT is still accepted as the gold standard. Material of the study was obtained by taking blood samples from cats who were admitted to the clinics between March 2016 and October 2016 in Ankara. Blood samples were inactivated and measurements were done with SFDT 1/4, 1/16, 1/64, 1/256, 1/1024 titers. Results: SFDT resulted positive in 56 (43.4%) cats at a dilution of 1/16, in 7 (5.4%) cats at a dilution of 1/64, in 23 (17.8%) cats at a dilution of 1/256 and negative in 43 (33.3%) cats. Comparison of demographic data with SFDT results showed that positive test results did not differ according to gender and age (P=0.803 and P=0.991, respectively). Seropositivity was higher in stray cats than house cats (P

Özet

Amaç: Toksoplasmozis, zorunlu hücre içi protozoon olan Toxoplasma gondii’nin (T.gondii) etken olduğu, tüm dünyada yaygıngörülebilen ve tüm vertebralıları tutabilen multisistemik bir hastalıktır. T. gondii için bilinen tek kesin konak Felidae ailesininüyeleridir. Çalışmamızda Ankara’da kedilerde Sabin-Feldman Dye Testi (SFDT) ile anti-Toxoplasma gondii antikorlarının tespitiamaçlanmıştır. Aynı bölgede daha önceden yapılan çalışmalar ile karşılaştırmalar yapılarak Toxoplasmozun yayılımı açısındangünümüzdeki durumun değerlendirilmesi hedeflenmiştir.Yöntemler: Çalışmamızda kullanılan Toxoplasma Rh suşunun idamesi laboratuvarımızda sağlanmaktadır. T.gondiitanımlanmasında kullanılan SFDT serolojik bir testtir ve altın standart olarak kabul edilmektedir. Çalışmanın materyali Mart2016 - Ekim 2016 tarihleri arasında Ankara’da kliniklere müracaat eden kedilerden kan örnekleri alınarak sağlanmıştır. Kedilerdenalınan kan örnekleri inaktive edilerek SFDT 1/4, 1/16, 1/64, 1/256, 1/1024 titrelerde çalışılmıştır.Bulgular: Toxoplasma gondii araştırması yapılan kedilerin 56’sında (%43,4) SFDT 1/16 titrede, 7’sinde (%5,4) 1/64 titrede,23’ünde (%17,8) 1/256 titrede pozitif saptanırken, 43’ünde (%33,3) negatif çıkmıştır. Demografik bilgiler ile SFDT sonuçlarınınkarşılaştırılmasında; pozitif test sonuçlarının cinsiyet ve yaş ile ilişki göstermediği bulunmuştur (Sırasıyla P=0,803 ve P=0,991).Sokak kedilerinde ev kedilerine göre seropozitiflik fazladır (P

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Cite This Article

KOÇ, N., GÜRÜZ, Y., YÜCESAN, B., KILIÇ, S., SEZEN, F., BABÜR, C. (2022). Investigation of Anti-Toxoplasma gondii Antibodies in Cats Using Sabin- Feldman Dye Test in Ankara in 2016. Türkiye Parazitoloji Dergisi, Cilt: 43 - Sayı: 1, 5–9. https://ajindex.com/articles/630041

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