SavedAdd your journal

Journals / Türkiye Parazitoloji Dergisi / 2020 / Cilt: 44 - Sayı: 1

Molecular Prevalence and Phylogenetic Characterization of Enterocytozoon bieneusi in Healthy Cattle

Sağlıklı Sığırlarda Enterocytozoon bieneusi’nin Moleküler Prevalansı ve Filogenetik Karakterizasyonu

Mübeccel OKUR
Alparslan YILDIRIM
Tuğba BİLGİN
Sadullah ULUĞ
Gupse Kübra KARADEMİR
Gamze YETİŞMİŞ
Pages
36–42
Publication Date
2022-11-06
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: First molecular epidemiological data on E. bieneusi in cattle from Turkey were obtained with this study

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: In this study, it was aimed to determine the molecular prevalence and genotypes of Enterocytozoon in healthy cattle.
📊
Key Findings & Data:Methods: Fecal samples were collected from 50 cattle in Sivas between October 2017 and March 2018 and genomic DNA (gDNA) isolations were performed. gDNA isolates were processed by Nested PCR specifically amplifying ITS rRNA gene region to identify E. bieneusi.
💡
Conclusion & Impact:Conclusion: First molecular epidemiological data on E. bieneusi in cattle from Turkey were obtained with this study
🔬Design:Research Article
📍Scope:2017 Verileri
⚙️Variables:Domain Parameters

Abstract

Objective: In this study, it was aimed to determine the molecular prevalence and genotypes of Enterocytozoon in healthy cattle. Methods: Fecal samples were collected from 50 cattle in Sivas between October 2017 and March 2018 and genomic DNA (gDNA) isolations were performed. gDNA isolates were processed by Nested PCR specifically amplifying ITS rRNA gene region to identify E. bieneusi. ITS rRNA region of E. bieneusi positive isolates were sequenced for genotyping and phylogenetic analyzes. Obtained sequences were assembled with appropriative genetic software, then phylogenetic relationships were revealed. Results: According to Nested PCR analyses, 29 (19.3%) out of totally examined samples were found positive for E. bieneusi. As a result of the sequence analyses, five distinct genotypes were determined. The most frequent genotype ERUSS1 and the other ERUSS2-4 genotypes were characterized as close to each other, which was reported for the first time in the world. Two isolates were determined in N genotype that was reported from cattle in Germany and were more different from the other genotypes. Phylogenetic analysis revealed that all the genotypes characterized in the study belonged to the genogroup 2. Conclusion: First molecular epidemiological data on E. bieneusi in cattle from Turkey were obtained with this study

Özet

Amaç: Bu çalışmada sağlıklı sığırlarda Enterocytozoon bieneusi’nin moleküler prevalansının ve genotiplerinin belirlenmesiamaçlanmıştır.Yöntemler: Çalışmada, Ekim 2017 ve Mart 2018 tarihleri arasında Sivas yöresindeki sağlıklı görünümlü 150 sığırdan dışkıörnekleri toplanmış ve genomik DNA (gDNA) izolasyonları yapılmıştır. Elde edilen gDNA izolatları, E. bieneusi’nin identifikasyonuamacıyla, ITS rRNA gen bölgesini amplifiye eden Nested PZR ile işlenmiştir. E. bieneusi pozitif izolatların ITS rRNA gen bölgesiamplikonları genotiplendirme ve filogenetik analizler için sekanslanmıştır. Elde edilen sekanslar uygun genetik yazılımlarlaişlenerek genotipik yapıları ve sonrasında da filogenetik ilişkileri ortaya çıkarılmıştır.Bulgular: Nested PZR ile incelemesi yapılan örneklerden 29’u (%19,3) E. bieneusi yönünden pozitif bulunmuştur. Sekansanalizleri sonucu beş ayrı genotip belirlenmiştir. En yaygın genotip olarak bulunan ERUSS1 genotipi ile ERUSS2-4 genotipleriyakın genotipler olarak karakterize edilmiş ve ilk kez rapor edilmiştir. İki izolatın ise Almanya’da bir sığırdan rapor edilmiş olan Ngenotipinde olduğu belirlenmiş ve diğer genotiplerden daha farklı olduğu görülmüştür. Filogenetik analizler çalışmada karakterizeedilen tüm genotiplerin genogrup 2’de yer aldığını ortaya koymuştur.Sonuç: Bu çalışma ile Türkiye’de sığırlarda E. bieneusi enfeksiyonları üzerine ilk moleküler epidemiyolojik veriler elde edilmiştir.

🔖

Cite This Article

OKUR, M., YILDIRIM, A., BİLGİN, T., ULUĞ, S., KARADEMİR, G. K., YETİŞMİŞ, G. (2022). Molecular Prevalence and Phylogenetic Characterization of Enterocytozoon bieneusi in Healthy Cattle. Türkiye Parazitoloji Dergisi, Cilt: 44 - Sayı: 1, 36–42. https://ajindex.com/articles/629974

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Mathis A, Weber R, Deplazes P. Zoonotic potential of the microsporidia. Clin Microbiol Rev. 2005;18:423-445
  2. Vávra J, Lukeš J. Microsporidia and ‘the art of living together’. Adv Parasitol. 2013;82:253-319
  3. Keeling P. Five questions about microsporidia. PLoS Pathogens. 2009;5:e1000489
  4. Santin M, Fayer R. Microsporidiosis: Enterocytozoon bieneusi in domesticated and wild animals. Res Vet Sci. 2011;90:363-371
  5. Matos O, Lobo ML, Xiao L. Epidemiology of Enterocytozoon bieneusi Infection in Humans. J Parasitol Res. 2012;2012:981424
  6. Thellier M, Breton J. Enterocytozoon bieneusi in human and animals, focus on laboratory identification and molecular epidemiology. Parasite. 2008;15:349-358
  7. Santin M, Fayer R. Enterocytozoon bieneusi genotype nomenclature based on the internal transcribed spacer sequence: a consensus. J Eukar Microbiol. 2009;56:34-8
  8. Jiang Y, Tao W, Wan Q, Li Q, Yang Y, Lin Y, et al. Zoonotic and potentially host-adapted Enterocytozoon bieneusi genotypes in sheep and cattle in northeast China and an increasing concern about the zoonotic importance of previously considered ruminant-adapted genotypes. Appl Environ Microbiol. 2015;81:3326-3335
  9. Zhao W, Yu S, Yang Z, Zhang Y, Zhang L, Wang R, et al. Genotyping of Enterocytozoon bieneusi (Microsporidia) isolated from various birds in China. Infec Gen Evol. 2016;40:151-154
  10. Karim MR, Dong H, Li T, Yu F, Li D, Zhang L, et al. Predomination and new genotypes of Enterocytozoon bieneusi in captive nonhuman primates in zoos in China: high genetic diversity and zoonotic significance. PloS One. 2015;10:e0117991
  11. Deng L, Li W, Zhong Z, Gong C, Liu X, Huang X, et al. Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China. Parasit Vectors. 2016;9:561
  12. Li W, Deng L, Yu X, Zhong Z, Wang Q, Liu X, et al. Multilocus genotypes and broad host-range of Enterocytozoon bieneusi in captive wildlife at zoological gardens in China. Parasit Vectors. 2016;9:395
  13. Karim MR, Wang R, Dong H, Zhang L, Li J, Zhang S, et al. Genetic polymorphism and zoonotic potential of Enterocytozoon bieneusi from nonhuman primates in China. Appl Environ Microbiol. 2014;80:1893-8
  14. Li N, Xiao L, Wang L, Zhao S, Zhao X, Duan L, et al. Molecular surveillance of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi by genotyping and subtyping parasites in wastewater. PLoS Neg Trop Dis. 2012;6:e1809
  15. Jiang Y, Tao W, Wan Q, Li Q, Y