Journals / Türk Hijyen ve Deneysel Biyoloji Dergisi / 1999 / Cilt: 56 - Sayı: 3

Comparison of disc diffusion, agar screening and broth microdilution methods to identify methicillin-resistant Staphylococcus aureus

Staphylococcus aureus suşlarında metisilin direncinin saptanmasında disk difüzyon, agar tarama ve buyyon mikrodilüsyon yöntemlerinin karşılaştırılması

Pages
103–107
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The rate of resistance to methicillin in our hospital seems higher than other centers.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, methicillin resistance in nosocomial and community isolates of S. aureus was investigated by three different methods and the results were compared.
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Key Findings & Data:All methods displayed same rate of detection. 7 (%14.3) of 49 community acquired and 15 (%48.4) of 31 nosocomial isolates were methicillin resistant.
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Conclusion & Impact:The rate of resistance to methicillin in our hospital seems higher than other centers.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, methicillin resistance in nosocomial and community isolates of S. aureus was investigated by three different methods and the results were compared. Disc diffusion, agar screening and broth microdilution methods were carried out to detect the methicillin resistance according to the current guidelines of the NCCLS. All methods displayed same rate of detection. 7 (%14.3) of 49 community acquired and 15 (%48.4) of 31 nosocomial isolates were methicillin resistant. The rate of resistance to methicillin in our hospital seems higher than other centers.

Özet

Bu çalışmada hastane ve toplum kaynaklı S. aureus suşlarında metisilin direnci üç farklı yöntemle araştırılıp, sonuçları karşılaştırılmıştır. Metisilin direncinin araştırılmasında NCCLS'in önerdiği şekilde disk difüzyon, agar tarama ve buyyon mikrodilüsyon yöntemleri kullanılmıştır. Her üç yöntem de birbiriyle % 100 uyumlu bulunmuştur. 49 toplum kaynaklı suşun 7'si (%14.3) ve 31 hastane kaynaklı suşun 15'i (%48.4), metisiline dirençli olarak tanımlanmıştır. Hastanemizde MRSA oranı diğer çalışmalara göre yüksek bulunmuştur.

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Cite This Article

KESKİN, Y., TÜLEK, N., MERT, A. (2022). Comparison of disc diffusion, agar screening and broth microdilution methods to identify methicillin-resistant Staphylococcus aureus. Türk Hijyen ve Deneysel Biyoloji Dergisi, Cilt: 56 - Sayı: 3, 103–107. https://ajindex.com/articles/625286

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