As a result, a high correlation coefficient (r=0.8303) between the ATP bioluminescence assay and Escherichia coli 0157:H7 count was found as an acceptable value for such a study.
📌 3 Key Takeaways
🎯
Objective & Hypothesis:In this study, immunomagnetic separation and ATP bioluminescence methods have been combined and used in enumeration of the bacteria Escherichia coli 0157:H7 in sterile buffer solutions.
📊
Key Findings & Data:In this study, immunomagnetic separation and ATP bioluminescence methods have been combined and used in enumeration of the bacteria Escherichia coli 0157:H7 in sterile buffer solutions.
💡
Conclusion & Impact:As a result, a high correlation coefficient (r=0.8303) between the ATP bioluminescence assay and Escherichia coli 0157:H7 count was found as an acceptable value for such a study.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
In this study, immunomagnetic separation and ATP bioluminescence methods have been combined and used in enumeration of the bacteria Escherichia coli 0157:H7 in sterile buffer solutions. As a result, a high correlation coefficient (r=0.8303) between the ATP bioluminescence assay and Escherichia coli 0157:H7 count was found as an acceptable value for such a study.
Özet
Bu çalışmada; immunomanyetik ayırma yöntemi ile ATP biyolüminesans yöntemi birlikte kullanılarak, Escherichia coli 0157:H7 bakterisinin tampon ortamlarda sayımı yapılmıştır. Çalışma sonucunda, ATP biyolüminesans ölçümü ile Escherichia coli 0157:H7 sayımı arasında yüksek bir korelasyon katsayısı (r=0.8303) bulunmuştur.
🔖
Cite This Article
ÖZBAŞ, Z. Y., MERCANOĞLU, B., AYTAÇ, S. A. (2022). Enumeration of Escherichia coli 0157:H7 by using immunomagnetic separation and ATP bioluminescence in buffer solution. Türk Hijyen ve Deneysel Biyoloji Dergisi, Cilt: 58 - Sayı: 2, 49–52. https://ajindex.com/articles/625280
Özbaş ZY, Aytaç SA. Escherichia coli 0157:H7: Epidemiyolojisi, gıdalarla ilişkisi, patojenitesi ve izolasyon yöntemleri. Türk Hij ve Den Biyol Derg 1995; 52: 47-53
Bautista DA. ATP Bioluminescence. In: Robinson RK, Batt CA, Patel PD, eds. Encyclopedia of food microbiology. Great Britain: Academic Press, 1999: 80-8
Cutter CN, Dorsa WJ, Siragusa GR. A rapid microbial ATP bioluminescence assay for meat carcasses. Dairy, Food and Environmental Sanitation 1996; 16: 726-36
Anonymous Dynabeads magnetic separation. Cells and proteins, nucleic acids, microorganisms. Dynal Product Catalogue, Oslo, 1998: 5
Poggemann HM, Baumgart J. Hygiene monitoring by ATP-determination with the Hy-LiteTM system.Fleischwirtschaft 1996; 76: 272-3
Waes G, Van Crombrugge J, Reybroeck W. The ATP-F test for estimation of bacteriological quality of raw milk.Modern Microbiological Methods for Dairy Products, IDF, Brussels, 1989: 279-86.