SavedAdd your journal

Journals / Türk Hijyen ve Deneysel Biyoloji Dergisi / 2013 / Cilt: 70 - Sayı: 2

Investigation of cross-reactions with Francisella tularensis antibodies to Brucella

Francisella tularensis antikorları ile Brucella çapraz reaksiyonlarının araştırılması

Burak ÇİTİL
Yasemin BAYRAM
Selçuk KILIÇ
Bekir ÇELEBİ
Pages
65–70
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: The cross-reaction titers determined with MA test in both infections were reduced to 40 or less by 2-ME, suggesting that the titers are due to immunoglobulin M antibody.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Cross-reactions with Francisella tularensis antibodies to Brucella, Legionella, Yersinia, and Mycoplasma species have been described.
📊
Key Findings & Data:Method: The aim of this study was to assess cross reactions and whether IgM or IgG antibodies are responsible for Brucella and F.tularensis cross- reactions, by the use of microagglutination,Brucella ELISA and 2-mercaptoethanol microagglutination test Results: Cross-reaction agglutinin titers to B.abortus antigen were found in 49 of 260 (18.8%) tularemia positive serum specimens, and cross-reaction titers to F.tularensis antigen were found in 23 of 252 (9.1%) Brucella positive serum specimens.
💡
Conclusion & Impact:Conclusion: The cross-reaction titers determined with MA test in both infections were reduced to 40 or less by 2-ME, suggesting that the titers are due to immunoglobulin M antibody.
🔬Design:Research Article
📍Scope:13 samples
⚙️Variables:Domain Parameters

Abstract

Objective: Cross-reactions with Francisella tularensis antibodies to Brucella, Legionella, Yersinia, and Mycoplasma species have been described. F.tularensis LPS is able to induce serological cross- reactions indistinguishable from brucellosis due to a similar immunodominant epitope in the Brucella O-polysaccharide. In agglutination tests, cross-reaction occurred only between F.tularensis and Brucella abortus, cross reactivity with Brucella can confuse interpretation of the results. Method: The aim of this study was to assess cross reactions and whether IgM or IgG antibodies are responsible for Brucella and F.tularensis cross- reactions, by the use of microagglutination,Brucella ELISA and 2-mercaptoethanol microagglutination test Results: Cross-reaction agglutinin titers to B.abortus antigen were found in 49 of 260 (18.8%) tularemia positive serum specimens, and cross-reaction titers to F.tularensis antigen were found in 23 of 252 (9.1%) Brucella positive serum specimens. While thirty six (73.5%) tularemia positive serum titers had Brucella cross reactivity at the titers of 1:20-1:80, cross-reaction with ≥1:160 titers was found in 13 samples (26.5%). The cross-reaction titers to B.abortus antigen were reduced to equal or less 1:40 titers by 2-ME. Cross- reactivity at the titers of 1:20-1:80 was determined in 22 of 23 Brucella positive samples. Conclusion: The cross-reaction titers determined with MA test in both infections were reduced to 40 or less by 2-ME, suggesting that the titers are due to immunoglobulin M antibody.

Özet

Amaç: Francisella tularensis antikorları ile Brucella, Legionella, Yersinia ve Mycoplasma türleri arasında çapraz reaksiyonlar bildirilmiştir.F.tularensis liposakkarit yapısında bulunanBrucellaO-polisakkaritindeki benzer antijenik yapılar serolojiktestlerde çapraz reaksiyonlara neden olabilmektedir.Aglütinasyon testlerinde F.tularensis ve Brucella abortus arasındaki çapraz reaksiyonlar sonuçlarınyorumlanmasını güçleştirebilir.Yöntem: Brucella ve F.tularensis arasındaki çapraz reaksiyonların sıklığı ve bu reaksiyonlardan sorumluolan antikorlar mikroaglütinasyon, Brucella ELISAve 2-merkaptoetanol mikroaglütinasyon testleri ilearaştırılmıştır.Bulgular: 260 tularemi pozitif örneğin 49 (%18.8)’unda B.abortus antijeniyle çapraz reaksiyonlar saptanırken, bruselloz pozitif 252 örneğin 23(%9.1)’ünde F.tularensis antijeni ile çapraz reaksiyongözlenmiştir. Otuzaltı tularemi (%73.5) pozitif serumdaBrucella çapraz reaksiyon titreleri 1:20-1:80 iken, 13örnekte (%26.5) ise ≥1:160 titrelerde çapraz reaksiyongözlenmiştir. 2-ME testi ile

🔖

Cite This Article

ÇİTİL, B., BAYRAM, Y., KILIÇ, S., ÇELEBİ, B. (2022). Investigation of cross-reactions with Francisella tularensis antibodies to Brucella. Türk Hijyen ve Deneysel Biyoloji Dergisi, Cilt: 70 - Sayı: 2, 65–70. https://ajindex.com/articles/625133

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (18)

  1. Kılıç S. A General Overview of Francisella tularensis and the Epidemiology of Tularemia in Turkey. Flora, 2010; 15(2): 37-58
  2. Kılıç S, Yeşilyurt M. Tularemi: Güncel Tedavi Seçeneklerine Genel Bir Bakış. Klimik Dergisi, 2011; 24(1): 2-10
  3. Yüce A, Çavuş SA. Türkiye'de Bruselloz: Genel Bakış. Klimik Dergisi, 2011; 19(3): 87-97
  4. Francis E, Evans AC. Agglutination, cross agglutination, and agglutin absorption in tularemia. Public Health Rep, 1926; 41: 1273-95
  5. Ransmeier JC, Ewing CL. The agglutination reaction in tularemia. J Infect Dis, 1941;69: 193–205
  6. Sjoested A. Genus I. Francisella. In: George MG, eds. Bergey’s Manual of Systematic Bacteriology, Vol 2, 2005: 200-9
  7. Corbel MJ. Recent advances in brucellosis. J Med Microbiol, 1997; 46(2): 101-3
  8. Stanfield CA, Taylor PW, Morgan HR. Some important antigenic relationships in the serological diagnosis of brucellosis. Am J Clin Pathol, 1952; 22: 211-7
  9. Behan KA, Klein GC. Reduction of Brucella species nd Francisella tularensis cross-reacting agglutinins by dithiothreitol. J Clin Microbiol, 1982; 16(4): 756–7
  10. Saslaw S, Carlisle HN. Studies with tularemia vaccines in volunteers. IV. Brucella agglutinins in vaccinated and non-vaccinated volunteers challenged with Pasteurella tularensis. Am J Med Sci, 1961; 242: 166–72
  11. Hornick RB. Tularemia. In: Evans AS, Brachman PS, eds. Bacterial Infections of Humans- Epidemiology and Control. New York. Plenum Medical Book Company, 1998: 823-37
  12. WHO guidelines on Tularaemia. Geneva: WHO Press, 2007
  13. Koskela P, Salminen A. Humoral immunity against Francisella tularensis after natural infection. J Clin Microbiol, 1985; 22(6): 973–9
  14. Lindquist D, Chu MC, Probert WS. Francisella and Brucella. In: Murray PR, Baron EJ, Jorgensen JH, eds. Manual of Clinical Microbiology, 9th ed. Washington. American Society for Microbiology Press, 2007: 815-34
  15. Tarnvik A, Berglund L. Tularemia. Eur Respir J. 2003; 21: 361-73
  16. Ulu Kılıç A, Kılıç S, Şencan I et al. İç Anadolu Bölgesinde Francisella tularensis alt tür halorctica’ya bağlı su kaynaklı bir Tularemi salgını. Mikrobiyol Bul, 2011; 45 (2): 234-47
  17. Marrodan T, Nenova-Poliakova R, Rubio M, Ariza J, Clavijo E, Smits HL, Diaz R. Evaluation of three methods to measure anti-Brucella IgM antibodies and interference of IgA in the interpretation of mercaptan-based tests. J Med Microbiol, 2001; 50: 663-6
  18. Ericsson M, Sandstrom G, Sjostedt A,Tarnvik A. Persistence of cell-mediated immunity an