Journals / Türk Hijyen ve Deneysel Biyoloji Dergisi / 2017 / Cilt: 74 - Sayı: 4

Investigation of the presence of rickettsiae in ticks parasitizing on humans in Çorum region

Çorum yöresinde insanlar üzerinde parazitlenen kenelerde riketsiya varlığının araştırılması

Pages
293–298
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Therefore, it suggested that the healthcare staff working in this region should be careful against the possibiliy that tick-borne rickettsioses may develop in people infected by tick in Çorum region

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this study is to determine the rickettsiae in ticks collected on human in Çorum Province by using the Polymerase Chain Reaction (PCR).Methods: A total of 1010 tick samples which were collected on humans identified to species level in terms of the morphological characters.
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Key Findings & Data:Total DNA’s individually extracted from ticks were screened for the presence of Spotted Fever Group rickettsiae using the PCR targeting rickettsial citrate synthase (gltA, 381 bp) and outer membrane protein (ompA, 532 bp) genes.Results: Out of 741 Hyalomma marginatum ticks collected from humans in Çorum Province, 51 (6.88%) were infected Rickettsia aeschlimannii, 3 (0.4%) were infected Rickettsia sibirica mongolitimonae.
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Conclusion & Impact:Therefore, it suggested that the healthcare staff working in this region should be careful against the possibiliy that tick-borne rickettsioses may develop in people infected by tick in Çorum region
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study is to determine the rickettsiae in ticks collected on human in Çorum Province by using the Polymerase Chain Reaction (PCR).Methods: A total of 1010 tick samples which were collected on humans identified to species level in terms of the morphological characters. Total DNA’s individually extracted from ticks were screened for the presence of Spotted Fever Group rickettsiae using the PCR targeting rickettsial citrate synthase (gltA, 381 bp) and outer membrane protein (ompA, 532 bp) genes.Results: Out of 741 Hyalomma marginatum ticks collected from humans in Çorum Province, 51 (6.88%) were infected Rickettsia aeschlimannii, 3 (0.4%) were infected Rickettsia sibirica mongolitimonae. Out of 32 Dermacentor marginatus ticks, 3 (9.4%) were infected Rickettsia raoultii and 3 (9.4%) were infected Rickettsia slovaca. In addition, rickettsial DNA was not found in Hyalomma aegyptium, Hyalomma excavatum, Hyalomma parva, Haemaphysalis punctata, Ixodes ricinus, Rhipicephalus bursa and Rhipicephalus turanicus ticks.Conclusion: In the present study, rickettsiae in ticks collected from Çorum Province were examined for the first time. According to our results, 4 different rickettsiae, R. aeschlimannii, R. sibirica mongolitimonae, R. raoultii and R. slovaca were commonly found in these ticks. Therefore, it suggested that the healthcare staff working in this region should be careful against the possibiliy that tick-borne rickettsioses may develop in people infected by tick in Çorum region

Özet

Amaç: Bu çalışmada, Çorum yöresinde insanlarda parazitlenen kenelerde riketsiya varlığının Polimeraz Zincir Reaksiyonu (PZR) yöntemiyle araştırılması amaçlanmıştır. Yöntem: Çorum yöresinde insanlar üzerinden toplanan 1.010 adet kene toplanarak morfolojik karakterlerine göre tür teşhisleri yapılmıştır. Bu örneklerden bireysel olarak elde edilen total DNA'lar riketsiyal sitrat sentaz (gltA, 381 bp) ve dış membran protein A (ompA, 532 bp) gen bölgelerini hedefleyen primer setleri kullanılarak PZR yöntemi ile taranmıştır. Bulgular: Çorum ilinde insanlar üzerinden toplanan 741 Hyalomma marginatum örneğinin 51 (%6,88)'inde Rickettsia aeschlimannii, 3 (%0,4)'ünde Rickettsia sibirica mongolitimonae; 32 Dermacentor marginatus örneğinin 3 (%9,4)'ünde Rickettsia raoultii, 3 (%9,4)'ünde Rickettsia slovaca varlığı tespit edilmiştir. Çalışma kapsamında incelenen Hyalomma aegyptium, Hyalomma excavatum, Hyalomma parva, Haemaphysalis punctata, Ixodes ricinus, Rhipicephalus bursa ve Rhipicephalus turanicus türlerine ait kenelerde riketsiyal DNA varlığına rastlanmamıştır.Sonuç: Bu çalışmada, Çorum yöresindeki kenelerde riketsiya varlığı ilk kez araştırılmış ve R. aeschlimannii, R. sibirica mongolitimonae, R. raoultii ve R. slovaca olmak üzere dört farklı patojenik riketsiyanın bölge kenelerinde yaygın olarak bulunduğu tespit edilmiştir. Bu nedenle, Çorum yöresinde keneler tarafından enfeste edilen kişilerde kene kaynaklı riketsiyozların gelişebileceği ihtimaline karşı bölge sağlık personelinin dikkatli olması tavsiye edilmektedir.

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Cite This Article

KUL-KÖPRÜLÜ, T., BURSALI, A., TEKIN, Ş., KESKIN, A., KESKIN, A. (2022). Investigation of the presence of rickettsiae in ticks parasitizing on humans in Çorum region. Türk Hijyen ve Deneysel Biyoloji Dergisi, Cilt: 74 - Sayı: 4, 293–298. https://ajindex.com/articles/625004

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