SavedAdd your journal

Journals / Türkderm-Deri Hastalıkları ve Frengi Arşivi / 2006 / Cilt: 40 - Sayı: 1

The effect of narrowband UVB phototherapy on the expression of bcl-2 and p53: A preliminary study

Darbant UVB fototerapinin Bcl-2 ve P53 ekspresyonlarına etkisi: Pilot çalışma

Ayşe DURSUN
Nilsel İLTER
Özlem ERDEM
Esra ADIŞEN
Mehmet Ali GÜRER
Pages
23–26
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

CONCLUSIONS: High cumulative UVB doses could be neccessary to induce p53 and bcl-2 expression detectable by immunohistochemical staining and perhaps repetetive exposure to UVB is a better model for cutaneous photocarcinogenesis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:BACKGROUND: Carcinogenicity of ultraviolet B (UVB) therapy is driving attention increasingly.
📊
Key Findings & Data:Numerous genes that regulates apopitosis have been identified, among them p53 and bcl-2 are well defined.
💡
Conclusion & Impact:CONCLUSIONS: High cumulative UVB doses could be neccessary to induce p53 and bcl-2 expression detectable by immunohistochemical staining and perhaps repetetive exposure to UVB is a better model for cutaneous photocarcinogenesis.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

BACKGROUND: Carcinogenicity of ultraviolet B (UVB) therapy is driving attention increasingly. Numerous genes that regulates apopitosis have been identified, among them p53 and bcl-2 are well defined. In this study, we aimed to document the impact of the increasing cumulative doses on p53 and bcl-2 expression in order to define a relationship between narrowband UVB and carcinogenesis.METHODS: 42 patients receiving narrowband UVB treatment for various primary cutaneous disorders were enrolled in the study. Avidin-biotin-peroxidase method was used for the immunohistochemical evaluation. Nuclear staining for p53 and cytoplasmic staining for bcl-2 were evaluated as positive. RESULTS: No correlation was found between the cumulative doses of NB-UVB and p53 expression. The statistical analysis between the cumulative doses of NB-UVB and bcl-2 expression showed a weak correlation and this was not statistically significant. CONCLUSIONS: High cumulative UVB doses could be neccessary to induce p53 and bcl-2 expression detectable by immunohistochemical staining and perhaps repetetive exposure to UVB is a better model for cutaneous photocarcinogenesis.

Özet

Ultraviyole B (UVB)'nin karsinojenik etkisi giderek daha çok ilgi çekmektedir. Apopitozisi regüle eden genler içinde en önemlileri p53 ve bcl-2'dir. Biz bu çalışmada, UVB ve kutanöz fotokarsinogenez arasındaki olası ilişkiyi tespit edebilmek için kümülatif UVB dozlarının p53 ve bcl-2 ekspresyonlarına etkisini araştırdık. Çalışma grubunu çeşitli primer kutanöz hastalıklar için darbant-UVB fototerapisi alan 42 hasta oluşturdu. İmmünohistokimyasal değerlendirme için Avidin-biyotin-peroksidaz metodu kullanıldı. p53 için nükleer boyanma ve bcl-2 için ise sitoplazmik boyanma esas alındı.Kümülatif darbant-UVB dozlarıyla p53 ekspresyonları arasında istatistiksel olarak anlamlı bir ilişki saptanmadı. Kümülatif darbant UVB dozlarıyla ile bcl-2 ekspresyonları arasında istatistiksel olarak anlamlı olmayan zayıf bir korelasyon tespit edildi.Biz bu sonuçlarla, p53 ve bcl-2 gen ekspresyonlarının immünohistokimyasal olarak tespit edilmesi için daha yüksek kümülatif dozların gerekli olduğunu ya da tekrarlayan UVB dozlarının kutanöz karsinogenezisi değerlendirmede daha iyi bir model oluşturabileceğini düşünmekteyiz.

🔖

Cite This Article

DURSUN, A., İLTER, N., ERDEM, Ö., ADIŞEN, E., GÜRER, M. A. (2022). The effect of narrowband UVB phototherapy on the expression of bcl-2 and p53: A preliminary study. Türkderm-Deri Hastalıkları ve Frengi Arşivi, Cilt: 40 - Sayı: 1, 23–26. https://ajindex.com/articles/622159

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (16)

  1. Abel EA: Phototherapy: UVB and PUVA. Cutis 1999; 64: 339-342
  2. Hönigsmann H: Phototherapy for psoriasis. Clin Dermatol 2001; 26: 343-350
  3. Assefa Z, Garmyn M, Vantieghem A: Ultraviolet B radiation induced apopitosis in human keratinocytes: cytosolic activation of procaspase-8 and the role of bcl-2. FEBS Letters 1999; 540: 123- 132
  4. Nataraj AJ, Wolf P, Cerroni L, Ananthaswamy HN: P53 mutation in squamous cell carcinomas from psoriasis patients treated with psoralen+UVA (PUVA). J Invest Dermatol 1997; 109: 238-243
  5. Wang XM, McNiff JM, Klump V: An unexpected spectrum of p53 mutations from squamous cell carcinomas in psoriasis patients treated with PUVA. Photochem Photobiol 1997; 66: 294-299
  6. Isoherranen K, Sauroja I, Jansen C, Punnonen K: UV irradiation induces downregulation of bcl-2 expression in vitro and in vivo. Arch Dermatol Res 1999; 291(4): 212-216
  7. Mass P, Hoffmann K, Gambichler T, Altmeyer P, Mannherz HG: Premature keratinocyte death and expression of marker proteins of apopitosis in human skin after UVB exposure. Arch Dermatol Res 2003; 295(2): 71-79
  8. Soini Y, Kamel D, Paakko P: Aberrant accumulation of p53 associates with ki-67 and mitotic count in benign skin conditions. Br J Dermatol 1994; 131: 514-520
  9. Helander SD, Peters MS, Pittelkow MR: Expression of p53 protein in benign and malignant epidermal pathologic conditions. J Am Acad Dermatol 1993;29:741-748
  10. Washio F, Ueda M, Ito A, Ichihashi M: Higher susceptibility to apopitosis following ultraviolet B irradiation of xeroderma pigmentosum fibroblasts is accompanied by upregulation of p53 and downregulation of bcl-2. Br J Dermatol 1999; 140(6): 1031- 1037
  11. Moles JP, Moyret C, Guillot B: P53 gene mutations in human epithelial skin cancers. Oncogene 1993; 8: 583-588
  12. Sellheyer K, Krahl D, Ratech H: Distribution of bcl-2 and bax in embryonic and fetal human skin. Am J Dermatopathol 2001; 23: 1-7
  13. Wrone-smith T, Bergstrom J, Quevodo ME, Reddy V, Steril GC, Nickoloff B: Differential expression of cell survival and cdell cycle regulotory proteins in cutaneous squamoproliferative lesions. J Dermatol Sci 1999; 19: 53-67
  14. Wikonkal NM, Berg RJW, van Haselen CW: Bcl-2 vs p53 protein expression and apoptotic rate in human nonmelanoma skin cancers. Arch Dermatol 1997; 133: 599-602
  15. Morales-Ducret CRJ, van de Rijn M, Leprun DP: Bcl-2 expression in primary malignancies of the skin. Arch Dermatol 1995; 131: 909-912
  16. Mcdonnel TJ, Troncoso P, Brisbay SM: Expre