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Journals / Turkish Neurosurgery / 2018 / Cilt: 28 - Sayı: 4

Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin

Ece ERDAG
Nail IZGI
Alican TAHTA
Tugba BAGCI-ONDER
Cetin GENC
Yavuz ARAS
Pages
571–581
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Atorvastatin enhanced the therapeutic potency of NPCs, including neurological improvement.

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Objective & Hypothesis:AIM: To assess the efficacy of Neural progenitor cell (NPC) transplantation in ischemic stroke, and to investigate whether atorvastatinenhances therapeutic potency of NPC after stroke.MATERIAL and METHODS: The focal cerebral ischemia-reperfusion model was performed by transient occlusion of middlecerebral artery.
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Key Findings & Data:Adhesive removal, rotarod, cylinder tests, and magneticresonance imaging (MRI) were used for assessment of rats during 4 weeks.
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Conclusion & Impact:Atorvastatin enhanced the therapeutic potency of NPCs, including neurological improvement.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

AIM: To assess the efficacy of Neural progenitor cell (NPC) transplantation in ischemic stroke, and to investigate whether atorvastatinenhances therapeutic potency of NPC after stroke.MATERIAL and METHODS: The focal cerebral ischemia-reperfusion model was performed by transient occlusion of middlecerebral artery. Rats were assigned randomly to receive intracerebral transplantation of mouse NPC alone (mNPC), human NPCalone (hNPC), mouse NPC plus oral atorvastatin (mNPC+A), human NPC plus oral atorvastatin (hNPC+A), oral atorvastatin alone, orintracerebral Dulbecco’s Modified Eagle’s medium injection (control group). Adhesive removal, rotarod, cylinder tests, and magneticresonance imaging (MRI) were used for assessment of rats during 4 weeks. After sacrification on 28th day, rats were investigated byimmunofluorescent staining.RESULTS: The hNPC and mNPC groups showed significantly improved functional outcome and reduced infarct area ratio comparedwith the control group. The hNPC group had significantly better performance and lower infarct area ratio than the mNPC group.Addition of atorvastatin to stem cell therapy significantly improved functional outcome, although it did not affect the infarct area ratioon MRI. Anti-inflammatory response in the infarct area was higher in the mNPC group. NPC transplantation significantly reducedthe amount of microglia and a significant increase in the amount of astrocytes. CD8a+ T lymphocyte and granzyme B activities werenot detected in any of the subjects.CONCLUSION: Both hNPC and mNPC treatments significantly improved functional outcome, and reduced infarct area ratio afterstroke. Atorvastatin enhanced the therapeutic potency of NPCs, including neurological improvement.

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Cite This Article

ERDAG, E., IZGI, N., TAHTA, A., BAGCI-ONDER, T., GENC, C., ARAS, Y. (2022). Assessment of the MRI and Behavioral Test Results in a Focal Cerebral Ischemia-Reperfusion Model in the Rat after Separate and Combined Use of Mouse-Derived Neural Progenitor Cells, Human-Derived Neural Progenitor Cells and Atorvastatin. Turkish Neurosurgery, Cilt: 28 - Sayı: 4, 571–581. https://ajindex.com/articles/616965

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