Journals / Turkish Journal of Zoology / 2000 / Cilt: 24 - Sayı: supp

Histological and histochemical studies on the extensor muscle of the pectoal fin of rainbow trout (Oncorhynchus mykiss)

Gökkuşağı Alabalığı'nda (Oncorhynchus mykiss) pektoral yüzgecin ekstensör kası üzerinde histolojik ve histokimyasal çalışmalar

Pages
37–40
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, it was found that the diameter of red, intermediate and white fibres were 66.6 $mu$ m, 93.3 $mu$ m and 133.3 $mu$m, respectively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, taking the extensor muscle out of the pectoral fin of trout, lipid and mitochondria contents of muscle fibres were examined.
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Key Findings & Data:Succinic dehydrogenase and Sudan black B stain methods were applied on the sections removed by freezing microtomy.
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Conclusion & Impact:As a result, it was found that the diameter of red, intermediate and white fibres were 66.6 $mu$ m, 93.3 $mu$ m and 133.3 $mu$m, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, taking the extensor muscle out of the pectoral fin of trout, lipid and mitochondria contents of muscle fibres were examined. Succinic dehydrogenase and Sudan black B stain methods were applied on the sections removed by freezing microtomy. Haematoxylen and eosin staining were also applied on the sections obtained from paraffin blocks. It was observed histologically that nuclei of muscle fibre were located just under the sarcolemma. Three types of fibres red, intermediate and white were identified by the applied histochemical methods. It was observed that this muscle was rich in red and intermediate fibres. As a result, it was found that the diameter of red, intermediate and white fibres were 66.6 $mu$ m, 93.3 $mu$ m and 133.3 $mu$m, respectively.

Özet

Bu çalışmada, alabalığın pektoral yüzgecinden ekstensör kas çıkarılarak, kas liflerinin mitokondri ve lipid içerikleri incelenmiştir. Dondurma mikrotomuyla alınmış kesitlere, Suksinik dehidrogenaz ve Sudan siyahı B boyama yöntemleri uygulanmıştır. Aynı zamanda parafin bloklardan elde edilen kesitlere de Hematoksilen ve eozin boyaması yapılmıştır. Histolojik olarak enine kesitlerde, kas lifi çekirdeklerinin sarkolemmanın hemen altında yerleştiği gözlenmiştir. Uyguladığımız histokimyasal yöntemlerle kırmızı, ara ve beyaz olmak üzere üç lif tipi ayırtedilmiştir. Bu kasın kırmızı ve ara lif tipi yönünden zengin olduğu görülmüştür. Yapılan ölçümler sonucunda, kırmızı liflerin çapının 66.6 $mu$ m, ara liflerin çapının 93.3 $mu$ m ve beyaz liflerin çapının ise 133.3 $mu$ m olduğu bulunmuştur.

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Cite This Article

GEYİKOĞLU, F., ÖZKARAL, A. (2022). Histological and histochemical studies on the extensor muscle of the pectoal fin of rainbow trout (Oncorhynchus mykiss). Turkish Journal of Zoology, Cilt: 24 - Sayı: supp, 37–40. https://ajindex.com/articles/616669

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