Journals / Turkish Journal of Zoology / 2015 / Cilt: 39 - Sayı: 4

Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR RFLP

Pages
630–642
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These 3 A. mystacinusclades of Turkey were also supported by analysis of molecular variance (AMOVA) results, by revealing that genetic diversity was higheramong groups (79.55%) than within the groups.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The rocky mouse, Apodemus mystacinus, is a rodent species distributed in Anatolia.
📊
Key Findings & Data:A total of 108 specimens from 19 localitiesin Turkey were collected to study the molecular variation of A. mystacinus inferred from RFLP of mtDNA cytochrome b (cytb) andD-loop.
💡
Conclusion & Impact:These 3 A. mystacinusclades of Turkey were also supported by analysis of molecular variance (AMOVA) results, by revealing that genetic diversity was higheramong groups (79.55%) than within the groups.
🔬Design:Research Article
📍Scope:108 specimens
⚙️Variables:Domain Parameters

Abstract

The rocky mouse, Apodemus mystacinus, is a rodent species distributed in Anatolia. A total of 108 specimens from 19 localitiesin Turkey were collected to study the molecular variation of A. mystacinus inferred from RFLP of mtDNA cytochrome b (cytb) andD-loop. MboI, HaeIII, and RsaI from cytb digestion and MboI, Bf aI, and Hinf I from D-loop digestion showed differentiation among thestudied specimens. The UPGMA dendrogram of the combined data of cytb and D-loop indicated 3 main clades, A. m. euxinus, A. m.mystacinus, and A. m. smy rnensis, distributed in Turkey. The haplotype diversity observed in the present study was high. Furthermore,the fixation index values (FST) ranged from 0.75093 to 0.83384, which indicated high diversity among the clades. These 3 A. mystacinusclades of Turkey were also supported by analysis of molecular variance (AMOVA) results, by revealing that genetic diversity was higheramong groups (79.55%) than within the groups.

🔖

Cite This Article

ÇOLAK, E., ÇOLAK, R., OLGUN, G. K. (2022). Determination of genetic variations between Apodemus mystacinus populations distributed in Turkey inferred from mtDNA PCR RFLP. Turkish Journal of Zoology, Cilt: 39 - Sayı: 4, 630–642. https://ajindex.com/articles/616380

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (3)

  1. Bellinvia E (2004). A phylogenetic study of the genus Apodemus by sequencing the mitochondrial DNA control region. J Zool Syst Evol Res 42: 289–297. Bugarski-Stanojević V, Blagojević J, Adnaðević T, Jojić V, Vujośević M (2008). Molecular phylogeny and distribution of three Apodemus species (Muridae, Rodentia) in Serbia. J Zool Syst Evol Res 46: 278–286. Çıplak B (2003). Distribution of Tettigoniinae (Orthoptera, Tettigoniidae) bush-crickets in Turkey: the importance of the Anatolian Taurus Mountains in biodiversity and implications for conservation. Biodivers Conserv 12: 47–64. Çolak E, Yiğit N, Çolak R, Sözen M, Özkurt Ş, Kankılıç T (2004). Taxonomic status and distribution of Apodemus mystacinus (Danford and Alston, 1877) (Mammalia: Rodentia) in Turkey. Turk J Zool 28: 285–294. Çolak R, Çolak E, Yiğit N (2005). Morphometric, karyotypic, and electrophoretic analysis of the genus Apodemus Kaup, 1826 (Mammalia: Rodentia) in Thrace. Turk J Zool 29: 147–153. Çolak R, Kandemir İ, Çolak E, Yiğit N (2007).
  2. Esterase variation in rocky mouse, Apodemus mystacinus (Danford and Alston, 1877) (Mammalia: Rodentia) in Turkey. Acta Zool Bulgar 59: 47–52. Darvish J, Tarahomi M, Akbarirad S, Dianat M (2012). A new record of Allactaga euphratica from Ilam province, West of Iran. Iran J Animal Biosyst 8: 75–77. Deffontaine V, Libois R, Kotlik P (2005). Beyond the Mediterranean peninsulas: Evidence of Central European glacial refugia for a temperate forest mammal species, the bank vole (Clethrionomys glareolus). Mol Ecol 14: 1727–1739. Doyle JJ, Doyle JL (1991). Isolation of plant DNA from fresh tissue. Focus 12: 13–15. Ellerman JR (1948). Key to the rodents of southwest Asia in the British Museum collection. P Zool Soc Lond 118: 765–816. Ellerman JR, Morrison-Scott TCS (1951). Checklist of Palaearctic and Indian Mammals 1758–1946.
  3. London, UK: British Museum of Natural History. Excoffier L, Lischer HEL (2010). Arlequin suite ver 3.5: a new series of programs to perform population genetic analysis under Linux and Windows. Mol Ecol Resour 10: 564–567. Felsenstein J (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791. Filippucci MG, Macholán M, Michaux JR (2002). Genetic variation and evolution in the genus Apodemus (Muridae, Rodentia). Biol J Linn Soc 75: 395–419. Fu YX (1997). Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147: 915–925. Gherman CM, Sándor AD, Ka