Journals / Turkish Journal of Zoology / 2021 / Cilt: 45 - Sayı: 6

Molecular identification of eriophyoid mites in Thrace using the 28S and COI genes

Pages
479–485
Publication Date
2022-11-06
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Further studies are urgently needed to reveal genetic variation within and between eriophyoid species to make accurate species identification using molecular technics.Key words: Eriophyoidea, cytochrome c oxidase subunit I, 28S rRNA, DNA taxonomy

📌 3 Key Takeaways

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Objective & Hypothesis:Abstract: Eriophyoid mites are recognized as the second most economically important group among the Acari following spider mites.
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Key Findings & Data:In this study, we used DNA-based identification for five eriophyoid species collected from the Thrace region of Turkey (Aceria erinea, Rhyncaphytoptus ficifoliae, Eriophyes pyri, Aceria massalongoi, Colomerus vitis collected from Juglans regia L., Ficus carica L., Pyrus communis L., Vitex-agnus castus L., Vitis vinifera L., respectively) using both cytochrome oxidase subunit I (MW396744-MW396748) and 28S rRNA (MW396565-MW396571) in combination with morphological taxonomy.
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Conclusion & Impact:Further studies are urgently needed to reveal genetic variation within and between eriophyoid species to make accurate species identification using molecular technics.Key words: Eriophyoidea, cytochrome c oxidase subunit I, 28S rRNA, DNA taxonomy
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Abstract: Eriophyoid mites are recognized as the second most economically important group among the Acari following spider mites. The identification of eriopyhoid mites traditionally based on morphological characters. However, the size of these diagnostic structures is very small and this often causes misidentification of species. In this study, we used DNA-based identification for five eriophyoid species collected from the Thrace region of Turkey (Aceria erinea, Rhyncaphytoptus ficifoliae, Eriophyes pyri, Aceria massalongoi, Colomerus vitis collected from Juglans regia L., Ficus carica L., Pyrus communis L., Vitex-agnus castus L., Vitis vinifera L., respectively) using both cytochrome oxidase subunit I (MW396744-MW396748) and 28S rRNA (MW396565-MW396571) in combination with morphological taxonomy. A phylogenetic tree has also been constructed for each gene to get a deeper understanding of the evolutionary history of Eriophyoidea. Besides all the molecular data herein obtained are the first eriophyoidea sequences for the country, some of the sequences have been submitted to the public GenBank database for the first time. Further studies are urgently needed to reveal genetic variation within and between eriophyoid species to make accurate species identification using molecular technics.Key words: Eriophyoidea, cytochrome c oxidase subunit I, 28S rRNA, DNA taxonomy

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Cite This Article

ÖZDEMİR, E., İNAK, E., DENİZHAN, E. (2022). Molecular identification of eriophyoid mites in Thrace using the 28S and COI genes. Turkish Journal of Zoology, Cilt: 45 - Sayı: 6, 479–485. https://ajindex.com/articles/615928

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References (5)

  1. Amrine JW, Manson DCM (1996).
  2. Preparation, Mounting and Descriptive Study of Eriophyoid Mites. pp. 383-396. In: Lindquist, E. E., M. W. Sabelis and J. Bruin (Eds.). Eriophyoid Mites -Their Biology, Natural Enemies and Control. Elsevier. World Crop Pests 6, 785pp Amrine JW, Stasny TA, Flechtman, HWC (2003). Revised keys to world genera of Eriophyidea (Acari: Prostigmata). Indira Publishing House, West Bloomfield, MI. Arribas P, Andújar C, Moraza ML, Linard B, Emerson BC, Vogler AP (2020). Mitochondrial metagenomics reveals the ancient origin and phylodiversity of soil mites and provides a phylogeny of the Acari. Molecular biology and evolution 37 (3): 683-694. doi: 10.1093/molbev/msz255 Castalanelli MA, Severtson DL, Brumley CJ, Szito A, Foottit RG et al. (2010). A rapid non-destructive DNA extraction method for insects and other arthropods. Journal of Asia-Pacific Entomology 13 (3): 243-248. doi:10.1016/j.aspen.2010.04.003 Chetverikov PE, Cvrković T, Makunin A, Sukhareva S, Vidović B et al. (2015). Basal divergence of Eriophyoidea (Acariformes, Eupodina) inferred from combined partial COI and 28S gene sequences and CLSM genital anatomy. Experimental and Applied Acarology 67 (2): 219-245. doi: 10.1007/s10493-015-9945-9
  3. Cruickshank RH (2002). Molecular markers for the phylogenetics of mites and ticks. Systematic and Applied Acarology, 7 (1): 3-14. doi: 10.11158/saa.7.1.1 Dabert M (2006). DNA markers in the phylogenetics of the Acari. Biological Letters 43 (2): 97-107.
  4. De Lillo E, Craemer C, Amrine JW, Nuzzaci G (2009). Recommended procedures and techniques for morphological studies of Eriophyoidea (Acari: Prostigmata). In Eriophyoid Mites: Progress and Prognoses, Springer, Dordrecht. de Lillo E, Fanelli E, Valenzano D, Monfreda R, Troccoli A et al. (2020). Characterisation of Aceria massalongoi and a histopathological study of the leaf galls induced on chaste trees. Experimental and Applied Acarology 82 (1): 33-57. doi:10.1007/s10493-020-00518- x Denizhan E, Çobanoğlu S (2010). Van Gölü havzasında Ulmus campestris L. (Ulmaceae) üzerinde tespit edilen eriophyoid akarlar (Acari: Prostigmata: Eriophyoidea). Türkiye Entomoloji Dergisi 34 (4): 543-549.
  5. Denizhan E, Monfreda R, Cobanoglu S, de Lillo E (2006). Three new Aceria species (Acari: Eriophyoidea) from Turkey. International Journal of Acarology 32 (2): 179-184. doi: 10.1080/01647950608684458 Denizhan E, Monfreda R, De Lillo E, Cobanoglu S (2008). Two new species of eriophyoid mites (Acari: Eriophyoidea) ass