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Journals / Turkish Journal of Veterinary and Animal Sciences / 2001 / Cilt: 25 - Sayı: 5

Rabid identification of avian Salmonella isolates by air thermal cycler amplification and capillary gel electrophoresis

Kanatlı Salmonella izolatlarının air thermal cycler amplifikasyonu ve kapiller jel elektroforezi ile hızlı identifikasyonu

Vildan CANER
K. Tayfun ÇARLI
Ayşegül EYİGÖR
Pages
717–720
Publication Date
2022-11-04
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Thus, the genus-specific capillary PCR for the identification of a colony of Salmonellae from a selective agar plate was performed within approximately 2 h and the total time required for definitive diagnosis of infection was 2 days using primary enrichment (PE) and 7 days using delayed secondary enrichment (DSE).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The possible use of a genus-specific polymerase chain reaction (PCR) for the identification of Salmonella colonies was tested on 14 reference strains and 38 clinical Salmonella strains isolated from chickens by the standard method of the National Poultry Improvement Plan and Auxiliary Provisions (NPIP), USDA, U.S.A.
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Key Findings & Data:All the PCRs using primer pairs InvA1 and InvA2 (5) gave 281 bp amplification product specific for the Salmonella genus and produced results identical (100%) to those obtained with biochemical and serological methods.
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Conclusion & Impact:Thus, the genus-specific capillary PCR for the identification of a colony of Salmonellae from a selective agar plate was performed within approximately 2 h and the total time required for definitive diagnosis of infection was 2 days using primary enrichment (PE) and 7 days using delayed secondary enrichment (DSE).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The possible use of a genus-specific polymerase chain reaction (PCR) for the identification of Salmonella colonies was tested on 14 reference strains and 38 clinical Salmonella strains isolated from chickens by the standard method of the National Poultry Improvement Plan and Auxiliary Provisions (NPIP), USDA, U.S.A. All the PCRs using primer pairs InvA1 and InvA2 (5) gave 281 bp amplification product specific for the Salmonella genus and produced results identical (100%) to those obtained with biochemical and serological methods. Thus, the genus-specific capillary PCR for the identification of a colony of Salmonellae from a selective agar plate was performed within approximately 2 h and the total time required for definitive diagnosis of infection was 2 days using primary enrichment (PE) and 7 days using delayed secondary enrichment (DSE).

Özet

The possible use of a genus-specific polymerase chain reaction (PCR) for the identification of Salmonella colonies was tested on 14 reference strains and 38 clinical Salmonella strains isolated from chickens by the standard method of the National Poultry Improvement Plan and Auxiliary Provisions (NPIP), USDA, U.S.A. All the PCRs using primer pairs InvA1 and InvA2 (5) gave 281 bp amplification product specific for the Salmonella genus and produced results identical (100%) to those obtained with biochemical and serological methods. Thus, the genus-specific capillary PCR for the identification of a colony of Salmonellae from a selective agar plate was performed within approximately 2 h and the total time required for definitive diagnosis of infection was 2 days using primary enrichment (PE) and 7 days using delayed secondary enrichment (DSE).

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Cite This Article

CANER, V., ÇARLI, K. T., EYİGÖR, A. (2022). Rabid identification of avian Salmonella isolates by air thermal cycler amplification and capillary gel electrophoresis. Turkish Journal of Veterinary and Animal Sciences, Cilt: 25 - Sayı: 5, 717–720. https://ajindex.com/articles/615700

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References (1)

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