Journals / Turkish Journal of Veterinary and Animal Sciences / 2004 / Cilt: 28 - Sayı: 5

E. coli 0157:H7, E. coli ve doğal kontaminant koliformların kıymada ışınlama ve depolama sırasında ölüm kinetiği

Death kinetics of E. coli 0157: H7, E. coli and natural contaminant coliforms in minced beef during irradiation treatment and storage

Pages
915–920
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

There was no significant change in numbers of bacteria during frozen storage.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The death kinetics of Escherichia coli0157:H7, E. coli type 1 and total conforms after irradiation treatment in minced beef were evaluated to analyze the effect of increasing irradiation doses.
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Key Findings & Data:Irradiation doses ranging from 0.0 kGy to 1.5 kGy were evaluated for reducing numbers of E.coli during frozen storage conditions at -18 °C for 30 days.
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Conclusion & Impact:There was no significant change in numbers of bacteria during frozen storage.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The death kinetics of Escherichia coli0157:H7, E. coli type 1 and total conforms after irradiation treatment in minced beef were evaluated to analyze the effect of increasing irradiation doses. Irradiation doses ranging from 0.0 kGy to 1.5 kGy were evaluated for reducing numbers of E.coli during frozen storage conditions at -18 °C for 30 days. D10 values of E.coli0157:H7, E. coli type 1, and total coliforms were 0.245 kGy, 0.552 kGy and 0.293 kGy, respectively. An irradiation dose of 1.5 kGy was shown to inactivate 10sMPN/g of serotype 0157:H7and 103MPN/gof E.coli type 1. This inactivation level might be considered safe for the consumption of minced beef. Finally, E.coli type 1 was found to be a suitable indicator for assessing the impact of irradiation on E.coli 0157:H7 serotype. There was no significant change in numbers of bacteria during frozen storage.

Özet

Kıymada 0.0 - 1.5 kGy arasında uygulanan ışınlama sonrasında ve -18 °C'da 30 güne kadar yapılan depolama sırasında Escherichia coli 0157:H7 ve E. coli tip 1'in ölüm kinetiği incelenmiştir. E. coli 0157:H7, E. coli tip l ve doğal kontaminant koliformların D10 değerleri sırasıyla, 0.245 kGy, 0.552 kGy ve 0.293 kGy olarak belirlenmiştir. Bulgular, 1.5 kGy ışınlamanın 105EMS/g 0157:H7 serotipi ve 103EMS/g E.coli tip 1'i inaktive ettiğini ve kıymanın güvenilir bir biçimde tüketilebilmesi için bu inaktivasyon değerinin yeterli olduğunu, E. coli tip 1'in, E.coli 0157:H7 için iyi bir ışınlama indikatörü olduğunu göstermiştir. Donmuş depolama boyunca bakteri sayılarında istatistik açıdan önemli bir değişme olmamıştır.

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Cite This Article

HALKMAN, D. H. B. (2022). E. coli 0157:H7, E. coli ve doğal kontaminant koliformların kıymada ışınlama ve depolama sırasında ölüm kinetiği. Turkish Journal of Veterinary and Animal Sciences, Cilt: 28 - Sayı: 5, 915–920. https://ajindex.com/articles/615508

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References (14)

  1. Mariani-Kurkdjian, P. Bingen, E.: Escherichia coli 0157:H7, an emerging pathogen. Presse Medicale., 1999; 28: 2067-2074
  2. Noveir, M.R., Halkman, A.K.: A study on selective broths and agar media for the isolation of E. coli 0157:H7 serotype. Turk. J. Vet. Anim. Sci., 2000; 24: 459-464
  3. Morrison, D.M., Tyrrell, D.L.J., Jewell, L.D.: Colonic biopsy in verotoxin-induced hemorrhagic colitis and thrombotic thrombocytopenic purpura (TTP). Am. J. Clin. Pathol., 1986; 86: 108-112
  4. Ryan, C.A., Tauxe, R.V., Hosek, G.W., Wells, J.G., Stoesz, P.A., McFadden, H.W., Smith, P.W., Wright, G.F., Blake, P.A.: Escherichia coli 0157:H7 diarrhea in a nursing home: clinical, epidemiological, and pathological findings. J. Infect. Dis., 1986; 154: 631-638
  5. Vernozy-Rozand, C, Ray-Gueniot, S., Ragot, C, Bavai, C, Montet, M.P., Bouvet, J., Richard, Y.: Prevalence of Escherichia coli 0157:H7 in industrial minced beef. Lett. Appl. Microbiol., 2002:35:7-11
  6. Clavero, M.R., Monk, J.D. Beuchat, L.R., Doyle, M.P., Bracket, R.E.: Inactivation of Escherichia coli 0157:H7, Salmonella, Campylobacter jejuni in raw ground beef by gamma irradiation. Appl. Environ. Microbiol., 1994; 60: 2069-2075
  7. Çabalar, M., Boynukara, B., Gülhan, T., Ekin, H.: Prevalence of rotavirus, Escherichia coli K99, and 0157:H7 in healthy dairy cattle herds in Van, Turkey. Turk. J. Vet. Anim. Sci., 2001; 25: 191-196
  8. Walls, I., Scott, V.N.: Validation of predictive mathematical models describing growth of Staphylococcus aureus. J. Food Prot, 1996; 59: 11-15
  9. Buchanan, R.L., Bagi, L.K.: Effect of water activity and humectants identity on the growth kinetics of Escherichia coli 0157:H7. Food Microbiol., 1996; 14: 413-423
  10. Farkas, J.: Physical Methods of Food Microbiology. In: Doyle, M.P., Beuchat, L.R., Montville, TJ. Eds. Food Microbiology Fundamentals and Frontiers. American Society for Microbiology, Washington D.C. 1997; 497-519
  11. Lacroix, M., Quattara, B.: Combined industrial process with irradiation to assure inequity and preservation of food products- a review. Food Res. Int., 2000; 33: 719-724
  12. Hitchins, A.D., Feng, P., Watkins, W.D., Rippey, S.R., Chandler, L.A.: Escherichia coli and the Coliform Bacteria. Bacteriological Analytical Manual. 8th edn. Revision A. A0AC International, Gaithersburg, MD, USA, 2000; 4.01-4.29
  13. Satin, M.: Use of irradiation for microbial decontamination of meat: situation and perspectives. Meat Sci., 2002; 62: 277-283
  14. Thayer, D.W., Boyd, G.: Com