Journals / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 3

Ankara tavşanlarının perifer kan lökositlerinde alfa naftil asetat esteraz aktivitesinin belirlenmesi

Determination of alpha naphtyl acetate asterase activity in the peripheral blood leukocytes in Angora rabbits

Pages
881–884
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The ANAE positive lymphocyte rate was found to be 68.2%.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to determine the alpha naphthyl acetate esterase (ANAE) activity in the peripheral blood leukocytes in Angora rabbits.
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Key Findings & Data:The heparinized blood sample's obtained from 10 healthy adult Angora rabbits were used.
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Conclusion & Impact:The ANAE positive lymphocyte rate was found to be 68.2%.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to determine the alpha naphthyl acetate esterase (ANAE) activity in the peripheral blood leukocytes in Angora rabbits. The heparinized blood sample's obtained from 10 healthy adult Angora rabbits were used. ANAE stainings were applied at different pH values for the determination of ANAE activities. The ANAE positive reactions were observed in the T lymphocytes, monocytes, neutrophils, eosinophils and platelets. The ANAE positive lymphocyte rate was found to be 68.2%.

Özet

Bu çalışma Ankara tavşanlarının perifer kan lökositlerinde alfa naftil asetat esteraz aktivitesinin belirlenmesi amacıyla yapıldı. Çalışmada materyal olarak 10 adet sağlıklı ve erişkin Ankara tavşanlarından alınan heparinize kan örnekleri kullanıldı. ANAE aktivitesinin belirlenmesi için değişik pH' larda ANAE boyaması uygulandı. İncelenen preparatlarda T lenfosit, monosit, nötrofil ve eozinofil granulositler ile kan pulcuklarinda ANAE'ye karşı pozitif reaksiyona rastlandı. ANAE pozitif lenfosit oranı % 68,2 olarak saptandı.

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Cite This Article

ÖZCAN, Z. (2022). Ankara tavşanlarının perifer kan lökositlerinde alfa naftil asetat esteraz aktivitesinin belirlenmesi. Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 3, 881–884. https://ajindex.com/articles/615472

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References (15)

  1. Barret, T.J.: Basic immunology and its medical application. The C.V. Mosby Company, London. 1980; 48-65
  2. Fudenberg, H., Stites, D., Caldwell, J., Wells, V.: Basic clinical immunology. Lang Medical Publication, California. 1978; 78-95
  3. Higgy, K.E., Burns, G.F., Hayhoe, F.G.: Discrimination of B, T and Null lymphocytes by esterase cytochemistry. Scand. J. Haematol., 1977; 18: 437-448
  4. Kajikawa, 0., Koyama, H., Yoshikawa, T., Tsubaki, S., Saito, H.: Use of Alpha-naphthyl acetate esterase staining to identify T lymphocytes in cattle. Am. J. Vet. Res., 1983; 44: 1549-1552
  5. Knowles, D.M., Hoffman, T., Ferrarini, M., Kunkel, H.G.: The demonstration of acid alpha naphthyl acetate esterase activity in human lymphocytes usefulness as a T cell marker. Cell. Immun., 1978:35:112-123
  6. Mueller, J., Brundel, Re, G., Buerki, HL, Keller, H.U., Hess, M.W., Cottier, H.: Nonspecific acid esterase activity* A criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eur. J. Immunol., 1975; 5: 270-274
  7. Basso, G., Cocito, M.G., Samenzato, G., Pezzutto, A., Zanesco, L.: Cytochemical study of thymocytes and T lymphocytes. Br. J. Haematol., 1980; 44: 577-582
  8. Mueller, J., Keller, H.U., Hagmann, J.D., Cornidey, R.J., Ruchti, C, Cottier, H.: Nonspecific esterase in human lymphocytes. Int. Arch. Allergy. Appl., 1981; 64: 410-421
  9. Yang, T.J., Jantzen, P.A., Williams, LF.: Acid alpha naphthyl acetate esterase: presence of activity in bovine and human T and B lymphocytes. Immunology, 1979; 38: 85-92
  10. Kulenkampff, J., Janossy, G., Greaves, M.F.: Acid esterase in human lymphoid cells and leukaemic blasts: A marker for T lymphoctyes. Br. J. Haematol. 1977; 36: 231-240
  11. Ramos, J.A., Ramis, A.J., Marco, A., Domingo, M., Rabanal, R., Ferrer, L.: Histochemical and immunohistochemical study of the mucosal lymphoid system in swine. Am. J. Vet. Res., 1992; 53:1418-1426
  12. Osbaldiston, G.W., Sullivan, R.J.: Cytochemical demonstration of esterases in peripheral blood leucocytes. Am. J. Vet. Res., 1978; 39: 683-685
  13. Aştı, R.N., Alabay, B., Kurtdede, N., Altunay, H., Ergün, L: Farklı hayvan türlerinin perifer kan lökositlerinde alfa naftil asetat esteraz aktivitesinin belirlenmesi. Ankara Üniv. Vet. Fak. Derg., 1996:43: 129-133
  14. Maiti, N.K., Saini, S.S., Sharma, S.N.: Histochemical studies on chicken peripheral blood lymphocytes. Vet. Res. Commun., 1990;14: 207-210
  15. Knowles, D.M., Hoick, S.: Tissue localization of T- lymphocytes by the h