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Journals / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 2

Apseli sığır böbreklerinden Arcanobacterium (Actinomyces) pyogenes izolasyonu ve PCR ile identifikasyonu

Isolation of Arcanobacterium (Actinomyces) pyogenes from abscessed catle kidney and identification by PCR

Ayşe KILIÇ
Adile MUZ
Hasan Basri ERTAŞ
Gökben ÖZEY
Pages
455–459
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle.
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Key Findings & Data:A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected.
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Conclusion & Impact:It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.
🔬Design:Research Article
📍Scope:100 samples
⚙️Variables:Domain Parameters

Abstract

In this study, the presence of Arcanobacterium spp., which causes a variety of purulent infections involving the skin, joints and visceral organs, was investigated in abscessed kidney samples of cattle. A total of 500 cattle were examined at postmortem and 100 samples with abscess were collected. Arcanobacterium pyogenes was isolated in 40 (40%) of the samples examined. DNA extracted from the isolates were amplified by Polymerase Chain Reaction (PCR) using specific primers derived from plo gene of A. pyogenes and all the isolates were determined to be positive by PCR. It was concluded that PCR employed in this study may be applied for identification of A. pyogenes.

Özet

Bu çalışmada, deri, eklem ve iç organlarda çeşitli prulent infeksiyonlara sebep olan Arcanobacterium spp.'nin apseli sığır böbreklerindeki varlığı araştırıldı. Toplam 500 sığır postmortem olarak muayene edildi ve apseli olan 100 örnek toplandı. Arcanobacterium pyogenes 40 (% 40) numuneden izole edildi. Izolatlardan elde edilen DNA'lar A. pyogenes'in plo geninden türetilen spesifik primerler kullanılarak Polimeraz Zincir Reaksiyonunda (PZR) çoğaltıldılar ve tüm izolatların PZR'da pozitif olduğu saptandı. Bu sonuç çalışmada kullanılan PZR yönteminin A. pyogenes'in identifikasyonunda uygulanabileceğini göstermiştir.

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Cite This Article

KILIÇ, A., MUZ, A., ERTAŞ, H. B., ÖZEY, G. (2022). Apseli sığır böbreklerinden Arcanobacterium (Actinomyces) pyogenes izolasyonu ve PCR ile identifikasyonu. Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 2, 455–459. https://ajindex.com/articles/615460

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References (11)

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