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Journals / Turkish Journal of Veterinary and Animal Sciences / 2007 / Cilt: 31 - Sayı: 4

Preparation of neuraminidase- specific antiserum from the H9N2 subtype avian influenza virus

Ali Nazari SHIRVAN
Mehran MORADI
Mehdi AMINIAN
Rasool MADANI
Pages
219–223
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

No reaction was observed with neuraminidase-specific antiserum or H9-HA of the same virus.

📌 3 Key Takeaways

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Objective & Hypothesis:Abstract: Avian influenza virus stocks (A/chicken/Iran/259/1998/(H9N2)) were propagated in the allantoic cavities of 10-day-old embryonated chicken eggs.
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Key Findings & Data:The harvested suspension was concentrated by polyethylene glycol 6000.
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Conclusion & Impact:No reaction was observed with neuraminidase-specific antiserum or H9-HA of the same virus.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Abstract: Avian influenza virus stocks (A/chicken/Iran/259/1998/(H9N2)) were propagated in the allantoic cavities of 10-day-old embryonated chicken eggs. The harvested suspension was concentrated by polyethylene glycol 6000. Concentrated samples were layered onto sucrose gradient (30%-60%). Both hemagglutinin and neuraminidase were solubilized from purified viruses with Triton X-100 across 30% sucrose gradient. NA was isolated from HA and other viral proteins by affinity chromatography on N-(paminophenyl) oxamic acid. Fractions that had high NA activity and did not show HA activity were pooled and analyzed by neuraminidase inhibition and SDS-PAGE. No viral protein bands were detected, except for a single band in the position of NA. NA activity of purified protein was 3.8 x 104 NA units. Enzymatic activity of neuraminidase purified by this procedure decreased sharply above 48 °C. For preparation of antisera, rabbits were immunized with purified NA and Freund's adjuvant at 3-week intervals, and sera were collected 7 days after boosting. The purified neuraminidase produced a significant antibody response in agar gel precipitation. No reaction was observed with neuraminidase-specific antiserum or H9-HA of the same virus.

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Cite This Article

SHIRVAN, A. N., MORADI, M., AMINIAN, M., MADANI, R. (2022). Preparation of neuraminidase- specific antiserum from the H9N2 subtype avian influenza virus. Turkish Journal of Veterinary and Animal Sciences, Cilt: 31 - Sayı: 4, 219–223. https://ajindex.com/articles/615426

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