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Journals / Turkish Journal of Veterinary and Animal Sciences / 2007 / Cilt: 31 - Sayı: 4

Subklinik inek mastitis'lerinden izole edilen staphylococcus aureus suşlarinin koagulaz gen polimorfizmi

Coagulase gene polymorphism of staphylococcus aurreus isolated from subclinical bovine mastitis

Özkan ASLANTAŞ
Gökhan DOĞRUER
Cemil DEMİR
Yaşar EGÜN
Hülya TÜRÜTOĞLU
Zafer CANTEKİN
Pages
253–257
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.

📌 3 Key Takeaways

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Objective & Hypothesis:Abstract: This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur).
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Key Findings & Data:Amplification of the coagulase gene from 80 5. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons.
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Conclusion & Impact:The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Abstract: This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 5. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the studied regions and only 1 or 2 genotypes predominated.

Özet

Özet: Bu çalışma, üç farklı şehirden (Hatay, Gaziantep ve Burdur) toplanan mastitisli sığır sütlerinden izole edilen 80 Staphylococcus aureus susunun koagulaz gen polimorfizmini araştırmak amacıyla yapıldı. Seksen S. aureus susu 5 farklı polymerase chain reaction (PCR) amplikonu oluştururken, 79 izolat 1 amplikon ve 1 izolat 2 amplikon oluşturdu. İzolatlar, koagulaz geninin restriction fragment length polymorphism (RFLP) analizi ile 9 genotipe ayrıldı. İki yaygın genotip izolatların % 73,8'ini oluşturdu. Sonuçlar, çalışılan bölgelerde farklı genotiplerin olduğunu ve bunlardan sadece bir veya iki genotipin dominant olduğunu göstermektedir

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Cite This Article

ASLANTAŞ, Ö., DOĞRUER, G., DEMİR, C., EGÜN, Y., TÜRÜTOĞLU, H., CANTEKİN, Z. (2022). Subklinik inek mastitis'lerinden izole edilen staphylococcus aureus suşlarinin koagulaz gen polimorfizmi. Turkish Journal of Veterinary and Animal Sciences, Cilt: 31 - Sayı: 4, 253–257. https://ajindex.com/articles/615411

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References (13)

  1. Watts, J.L.: Etiological agents of bovine mastitis. Vet. Microbiol., 1988;16:41-66
  2. Kapur, V., Sischo, W.M., Greer, R.S., Whittam, T.S., Musser, J.M.: Molecular population genetic analysis of Staphylococcus aureus recovered from cows. J. Clin. Microbiol., 1995; 33: 376- 380
  3. Tenover, F.C., Arbeit, R., Archer, G., Biddle, J., Byrne, S., Goering, R., Hancock, G., Hebert, G.A., Hill, B., Hollis, R., Jarvis, W.R., Kreiswirt, B., Eisner, W., Maslow, J., McDougal, L.K., Miller, J.M., Mulligan, M., Pfaller, M.A.: Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J. Clin. Microbiol., 1994; 32: 407-415
  4. Rodrigues da Silva, E., da Silva, N.: Coagulase gene typing of Staphylococcus aureus isolated from cows with mastitis in southern Brazil. Can. J. Vet. Res., 2005; 69: 260-264
  5. Schlegelovâ, J., Dendis, M., Benedik J, Babâk, V., Rysânek, D.: Staphylococcus aureus isolates from dairy cows and humans on a farm differ in coagulase genotype. Vet. Microbiol., 2003; 92: 327-334
  6. Lange, C, Cardoso, M., Senczek, D., Schwarz, S.: Molecular subtyping of Staphylococcus aureus isolates from cases of bovine mastitis in Brazil. Vet. Microbiol., 1999; 67: 127-141
  7. Raimundo, 0., Deighton, M., Capstick, J., Gerraty, N.: Molecular typing of Staphylococcus aureus of bovine origin by polymorphisms of the coagulase gene. Vet. Microbiol.,-1999; 66: 275-284
  8. Annemdiler, C, Lâmmler, Ch., Zschöck, M.: Genotyping of Staphlycoccus aureus isolated from bovine mastitis. Vet. Microbiol., 1999; 69: 217-224
  9. Su, C, Herbelin, C, Frieze, N., Skardova, O., Sordillo, L.M.: Coagulase gene polymorphism of Staphylococcus aureus isolates from dairy cattle in different geographical areas. Epidemiol. Infect, 1999; 122: 329-336
  10. Hookey, J.V., Richardson, J.F., Cookson, B.D.: Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene. J. Clin. Microbiol., 1998; 36: 1083-1089
  11. Aarestrup, F.M., Dangler, C.A., Sordillo, L.M.: Prevalence of coagulase gene polymorphism in Staphylococcus aureus isolates causing bovine mastitis. Can. J. Vet. Res., 1995; 59: 124-128
  12. Goh, S.H., Byrne, S.K., Zhang, J.L., Chow, A.W.: Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. J. Clin. Microbiol., 1992; 30: 1642-1645
  13. Aarestrup, F.M., Scott, N.L., Sordillo, L.M.: Ability of Staphylococcus aureus coagulase genotypes to resist n