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Journals / Turkish Journal of Veterinary and Animal Sciences / 2016 / Cilt: 40 - Sayı: 4

Isolation of virulent Rhodococcus equi from Arabian horses in Şanlıurfa, Turkey

Sevil GÜRBİLEK ERDENLİĞ
Osman Yaşar TEL
Oktay KESKİN
Pages
417–420
Publication Date
2022-11-03
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Because of the existence of virulent R. equi in the environment, preventive measures should be taken against the risk of infection.

📌 3 Key Takeaways

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Objective & Hypothesis:The purposes of this study were to investigate plasmid profiles of the isolated virulent (VapA positive) agents by RFLP and to determine the quantitative aspects of Rhodococcus equi in the feces of adult horses and foals, as well as in soil.
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Key Findings & Data:The frequency of detection of R. equi and virulent R. equi in fecal samples and soil was studied from 20 farms in the Şanlıurfa region.
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Conclusion & Impact:Because of the existence of virulent R. equi in the environment, preventive measures should be taken against the risk of infection.
🔬Design:Research Article
📍Scope:315 samples
⚙️Variables:Domain Parameters

Abstract

The purposes of this study were to investigate plasmid profiles of the isolated virulent (VapA positive) agents by RFLP and to determine the quantitative aspects of Rhodococcus equi in the feces of adult horses and foals, as well as in soil. The frequency of detection of R. equi and virulent R. equi in fecal samples and soil was studied from 20 farms in the Şanlıurfa region. A total of 315 samples were examined. Nasal swabs from 25 foals with pneumonia were also analyzed. R. equi was isolated and identified from 263 (83.4%) samples originating from 15 out of 20 farms. From 105 samples each of soil and equine and foal feces, R. equi was isolated in 91 (86.6%), 94 (89.5%), and 78 (74.2%) samples, respectively. A total of 789 colonies isolated and identified as R. equi were examined by PCR for the VapA gene. As a result of PCR, 32 (4.0%) agents were identified as virulent R. equi. All virulent R. equi isolates contained 85 kb type-I plasmid. Additionally, R. equi could not be isolated from nasal swabs in foals with pneumonia. Because of the existence of virulent R. equi in the environment, preventive measures should be taken against the risk of infection.

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Cite This Article

ERDENLİĞ, S. G., TEL, O. Y., KESKİN, O. (2022). Isolation of virulent Rhodococcus equi from Arabian horses in Şanlıurfa, Turkey. Turkish Journal of Veterinary and Animal Sciences, Cilt: 40 - Sayı: 4, 417–420. https://ajindex.com/articles/615300

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