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Journals / Turkish Journal of Veterinary and Animal Sciences / 2016 / Cilt: 40 - Sayı: 2

High level fluoroquinolone resistance in Escherichia coli isolatedfrom animals in Turkey is due to multiple mechanisms

Figen ERSOY
Esra BÜYÜKCANGAZ
Songül SONAL
Pınar ŞAHİNTÜRK
Erdem ARSLAN
Ayşin ŞEN
Murat CENGİZ
A. Mark WEBBER
J V. Laura PIDDOCK
Pages
214–218
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results of this study show a wide range of different mechanisms of fluoroquinolone resistance in E. coli isolates in Turkey.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to determine the molecular mechanisms of fluoroquinolone resistance in E. coli isolated from cattle, goats, sheep, cats, and dogs in Turkey.
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Key Findings & Data:To identify phenotypic differences between isolates, the sum of the MIC values of 14 antimicrobials was calculated; values ranged from 565 to 2520 μg/mL, indicating the diversity in antimicrobial resistance present in the panel of isolates.
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Conclusion & Impact:The results of this study show a wide range of different mechanisms of fluoroquinolone resistance in E. coli isolates in Turkey.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to determine the molecular mechanisms of fluoroquinolone resistance in E. coli isolated from cattle, goats, sheep, cats, and dogs in Turkey. Twenty nonreplicate E. coli isolates (chosen on the basis of RAPD pattern) from food-producing animals were selected for the study. To identify phenotypic differences between isolates, the sum of the MIC values of 14 antimicrobials was calculated; values ranged from 565 to 2520 μg/mL, indicating the diversity in antimicrobial resistance present in the panel of isolates. PCR and qRT-PCR were used to characterize the presence and expression levels of known molecular mechanisms of fluoroquinolone resistance. The number of E. coli isolates having single, double, and triple topoisomerase mutations was 2, 10, and 5, respectively. Moreover, the number of qnrA - , qnrS - , oqxB -, and aac(6 )Ib-cr-containing E. coli isolates was 1, 4, 1, and 17, respectively. Increased expression of acrB and soxS was detected in 2 and 9 isolates, respectively. The results of this study show a wide range of different mechanisms of fluoroquinolone resistance in E. coli isolates in Turkey.

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Cite This Article

ERSOY, F., BÜYÜKCANGAZ, E., SONAL, S., ŞAHİNTÜRK, P., ARSLAN, E., ŞEN, A., CENGİZ, M., WEBBER, A. M., PIDDOCK, J. V. L. (2022). High level fluoroquinolone resistance in Escherichia coli isolatedfrom animals in Turkey is due to multiple mechanisms. Turkish Journal of Veterinary and Animal Sciences, Cilt: 40 - Sayı: 2, 214–218. https://ajindex.com/articles/615283

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