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Journals / Turkish Journal of Veterinary and Animal Sciences / 2016 / Cilt: 40 - Sayı: 4

Molecular characterization of the bovine noroviruses from diarrheic calves in Turkey

İbrahim SÖZDUTMAZ
Hakan IŞIDAN
İrem GÜLAÇTI
Pages
428–433
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, BNoV is one of the pathogens that contributes to neonatal calf diarrhea cases in Turkey.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, we performed a survey to detect bovine noroviruses (BNoVs) in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by nested reverse-transcription RT-PCR, using primers located in the consensus sequences of the BNoV RNA-dependent RNA polymerase (RdRp) gene.
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Key Findings & Data:The BNoV RdRp gene was detected in 1.7% (4/235) of samples by nested RT-PCR.
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Conclusion & Impact:In conclusion, BNoV is one of the pathogens that contributes to neonatal calf diarrhea cases in Turkey.
🔬Design:Survey & Empirical Study
📍Scope:2009 Verileri
⚙️Variables:Domain Parameters

Abstract

In this study, we performed a survey to detect bovine noroviruses (BNoVs) in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by nested reverse-transcription RT-PCR, using primers located in the consensus sequences of the BNoV RNA-dependent RNA polymerase (RdRp) gene. The BNoV RdRp gene was detected in 1.7% (4/235) of samples by nested RT-PCR. The nucleotide sequences of partial RdRp fragments from the BNoV isolates including the newly identified Turkish isolates showed more than 86.3% nt and 96.8% aa identity with each other. Interestingly, in a comparison of the nt and aa sequences, one of the Turkish BNoV strains was found to be 100% aa identical with some Italian and Tunisian strains. In conclusion, BNoV is one of the pathogens that contributes to neonatal calf diarrhea cases in Turkey.

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Cite This Article

SÖZDUTMAZ, İ., IŞIDAN, H., GÜLAÇTI, İ. (2022). Molecular characterization of the bovine noroviruses from diarrheic calves in Turkey. Turkish Journal of Veterinary and Animal Sciences, Cilt: 40 - Sayı: 4, 428–433. https://ajindex.com/articles/615281

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References (13)

  1. Ando T, Noel JS, Fankhauser RL. Genetic classification of Norwalk-like viruses. J Infect Dis 2000; 181: 336-348
  2. Green KY, Chanock RM, Kapikian AZ. Human caliciviruses. In: Knipe DM, Howley PM, editors. Fields Virology. Philadelphia, PA, USA: Lippincott Williams and Wilkins; 2001. pp. 841–874
  3. Jor E, Myrmel M, Jonassen CM. SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway. J Virol Methods 2010; 169: 1-7
  4. Oliver SL, Dastjerdi AM, Wong S, El-Attar L, Gallimore C, Brown DW, Green J, Bridger JC. Molecular characterization of bovine enteric caliciviruses: a distinct third genogroup of noroviruses (Norwalk-like viruses) unlikely to be of risk to humans. Virol J 2003; 77: 2789-2798
  5. Zaafrane MH, Kaplon J, Loulizi KS, Aouni Z, Pothier P, Aouni M, Balay KA. Molecular prevalence of bovine noroviruses and neboviruses detected in central-eastern Tunisia. Arch Virol 2012; 157: 1599-1604
  6. Woode GN, Bridger JC. Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. J Med Microbiol 1978; 11: 441-452
  7. Van der Poel WH, Van der HR, Verschoor F, Gelderblom H, Vinje J, Koopmans MP. Epidemiology of Norwalk-like virus infections in cattle in the Netherlands. Vet Microbiol 2003; 92: 297-309
  8. Dastjerdi AM, Green J, Gallimore CI, Brown DW, Bridger JC. The bovine Newbury agent-2 is genetically more closely related to human SRSVs than to animal caliciviruses. Virol J 1999; 254: 1-5
  9. Yilmaz H, Turan N, Altan E, Bostan K, Yilmaz A, Helps CR, Cho KO. First report on the phylogeny of bovine norovirus in Turkey. Arch Virol 2011; 156: 143-147
  10. Mauroy A, Scipioni A, Mathijs E, Saegerman C, Mast J, Bridger JC, Ziant D, Thys C, Thiry E. Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA. Vet Microbiol 2009: 137: 243-251
  11. Park SI, Jeong C, Kim HH, Park SH, Park SJ, Hyun BH, Yang DK, Kim SK, Kang MI, Cho KO. Molecular epidemiology of bovine noroviruses in South Korea. Vet Microbiol 2007; 124: 125-133
  12. Smiley JR, Hoet AE, Traven M, Tsunemitsu H, Saif LJ. Reverse transcription-PCR assays for detection of bovine enteric caliciviruses (BEC) and analysis of the genetic relationships among BEC and human caliciviruses. J Clin Microbiol 2003; 41: 3089-3099
  13. Kumar S, Stecher G, Peterson D, Tamura K. MEGA-CC: Computing core of Molecular Evolutionary Genetics Analysis program fo