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Journals / Turkish Journal of Veterinary and Animal Sciences / 2014 / Cilt: 38 - Sayı: 3

Te efects of vincristine sulfate on expression of galectin-3, Bcl-2, and carbohydrate structures specifc for EEL, GSL-1, and RCA-1 lectins in bitches with naturally occurring canine transmissible venereal tumor

Hasan AKŞİT
Ender UZABACI
Deniz NAK
Yavuz NAK
Kamil SEYREK
Gözde ŞİMŞEK
Musa Özgür ÖZYİĞİT
Sevda ÖZTÜRKOĞLU İNAN
Pages
331–338
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Tese results show that galectin-3, Bcl-2, and lectins (EEL, GSL-1, and RCA-1) are suitable indicators/markersfor CTVT tumor regression afer treatment with vincristine sulfate.

📌 3 Key Takeaways

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Objective & Hypothesis:Vincristine sulfate is one of the most efective chemical agents used in canine transmissible venereal tumor (CTVT)chemotherapy.
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Key Findings & Data:Te present study was designedto investigate vincristine sulfate treatment and the relationship between CTVT tumor regression and apoptosis [estimated by TdT-mediated dUTP-biotin nick end labeling (TUNEL)], galectin-3 (H-160), Bcl-2 (N-9), and lectin [EEL (B-135), GSL I (L-1100), andRCA I (BA-0084)] expression using immunohistochemical staining in 20 bitches with naturally occurring CTVTs.
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Conclusion & Impact:Tese results show that galectin-3, Bcl-2, and lectins (EEL, GSL-1, and RCA-1) are suitable indicators/markersfor CTVT tumor regression afer treatment with vincristine sulfate.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Vincristine sulfate is one of the most efective chemical agents used in canine transmissible venereal tumor (CTVT)chemotherapy. Its therapeutic efectiveness is pronounced and signifcant at the beginning of treatment. Te present study was designedto investigate vincristine sulfate treatment and the relationship between CTVT tumor regression and apoptosis [estimated by TdT-mediated dUTP-biotin nick end labeling (TUNEL)], galectin-3 (H-160), Bcl-2 (N-9), and lectin [EEL (B-135), GSL I (L-1100), andRCA I (BA-0084)] expression using immunohistochemical staining in 20 bitches with naturally occurring CTVTs. Evaluation wassemiquantitatively performed by HSCORE values using light microscopy. All observed staining was intracytoplasmic and increasedendothelial staining was noted with the Bcl-2 marker in tumor vessels. Apoptosis, TUNEL, and lectin scoring was higher in vincristinesulfate-treated tumors than in untreated tumors, while expression of antiapoptotic factors galectin-3 and Bcl-2 were lower in treatedthan in untreated CTVTs. Tese results show that galectin-3, Bcl-2, and lectins (EEL, GSL-1, and RCA-1) are suitable indicators/markersfor CTVT tumor regression afer treatment with vincristine sulfate.

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Cite This Article

AKŞİT, H., UZABACI, E., NAK, D., NAK, Y., SEYREK, K., ŞİMŞEK, G., ÖZYİĞİT, M. Ö., İNAN, S. Ö. (2022). Te efects of vincristine sulfate on expression of galectin-3, Bcl-2, and carbohydrate structures specifc for EEL, GSL-1, and RCA-1 lectins in bitches with naturally occurring canine transmissible venereal tumor. Turkish Journal of Veterinary and Animal Sciences, Cilt: 38 - Sayı: 3, 331–338. https://ajindex.com/articles/615262

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References (13)

  1. Stockmann D, Ferrari HF, Andrade AL, Cardoso TC, Luvizotto MCR. Detection of the tumour suppressor gene TP53 and expression of p53, Bcl-2 and p63 proteins in canine transmissible venereal tumour. Vet Comp Oncol 2011; 9: 251–259
  2. Nak D, Nak Y, Cangul IT, Tuna B. A clinico-pathological study on the efect of vincristine on transmissible venereal tumour in dogs. J Vet Med A Physiol Pathol Clin Med 2005; 52: 366–370 3. Rui K, Eimi S, Okamura T, Watanabe S, Hasegawa A. Expression of Bcl-2 in feline lymphoma cell lines. Vet Clin Pathol 2008; 37: 57–60
  3. Coli A, Bigotti G, Zucchetti F, Negro F, Massi G. Galectin-3, a marker of well-diferentiated thyroid carcinoma, is expressed in thyroid nodules with cytological atypia. Histopathology 2002; 40: 80–87
  4. Ishibashi H, Suzuki T, Suzuki S, Moriya T, Kaneko C, Takizawa T, Sunamori M, Handa M, Kondo T, Sasano H. Sex steroid hormone receptors in human thymoma, J Clin Endocrinol Metab 2003; 88: 2309–2317
  5. Özalp GR, Zik B, Bastan A, Peker S, Özdemir-Salci ES, Bastan I, Darbaz I, Salar S, Karakas K. Vincristine modulates the expression of Ki67 and apoptosis in naturally occurring canine transmissible venereal tumor (TVT). Biotech Histochem 2012; 87: 325–330
  6. Boscos CM, Ververidis HN. Canine TVT: clinical fndings, diagnosis and treatment. In: Proceedings of the WSAVA- FECAVA-HVMS World Congress. Rhodes, Greece: WSAVA- FECAVA-HVMS; 2004. pp. 758–761
  7. Cohen D. Te canine transmissible venereal tumor: a unique result of tumor progression. Adv Cancer Res 1985; 43: 75–112
  8. Pir Yağci İ, Kalender H. Bir erkek köpekte transmissible venereal tümör (TVT) olgusunun vincristine sulphate ile sağaltımı. Kafas Üniv Vet Fak Derg 2008; 14: 105–108 (in Turkish)
  9. Varughese EE, Singla VK, Ratnakaran U, Gandotra VK. Successful management of metastatic transmissible venereal tumour to skin of mammary region. Reprod Domest Anim 2012; 47: 366–369
  10. Jeon BS, Yoon BI. Altered expression of cellular Bcl-2 in the progression of hamster cholangiocarcinogenesis. ScientifcWorldJournal 2012; 2012: 385840
  11. Goping IS, Gross A, Lavoie JN, Nguyen M, Jemmerson R, Roth K, Korsmeyer SJ, Shore GC. Regulated targeting of Bax to mitochondria. J Cell Biol 1998; 143: 207–215
  12. Kirkin V, Joos S, Zörnig M. Te role of Bcl-2 family members in tumorigenesis. Biochim Biophys Acta 2004; 1: 229–249
  13. Eissa S, Labib R, Khalifa A, Swelam N, Khalil F, El-Shenawy AM. Regulators of apoptosis in human breast cancer. Clin Biochem 1999; 32: 321–32