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Journals / Turkish Journal of Veterinary and Animal Sciences / 2016 / Cilt: 40 - Sayı: 2

Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate

Caner ÖZTÜRK
Mustafa Numan BUCAK
Şükrü GÜNGÖR
Kenan ÇOYAN
Pınar AKALIN PEKER
Nuri BAŞPINAR
Pages
195–199
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to describe an optimal sonication procedure for sperm cells.
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Key Findings & Data:Therefore, we used several parameters such as damaged spermatozoa rate (%), mitochondrial activity rate (%), levels of lipid peroxidation, and total antioxidant potential.
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Conclusion & Impact:The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to describe an optimal sonication procedure for sperm cells. Therefore, we used several parameters such as damaged spermatozoa rate (%), mitochondrial activity rate (%), levels of lipid peroxidation, and total antioxidant potential. Ejaculates were collected from rams (n = 3) and were divided into aliquots and 3-, 6-, and 10-s duration times; 1, 3, 5, and 8 repetitive application groups were established. In the groups with 3-, 6- and 10-s duration times, with the increasing number of repeated applications, damaged spermatozoa rates increased (P < 0.05) while mitochondrial activity rates decreased (P < 0.05). In relation with sonication duration time, total antioxidant potential levels increased (P < 0.05) in single-application groups compared to those in control groups and gradually decreased as the repetitions increased. The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate.

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Cite This Article

ÖZTÜRK, C., BUCAK, M. N., GÜNGÖR, Ş., ÇOYAN, K., PEKER, P. A., BAŞPINAR, N. (2022). Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate. Turkish Journal of Veterinary and Animal Sciences, Cilt: 40 - Sayı: 2, 195–199. https://ajindex.com/articles/615088

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References (16)

  1. Çoyan K, Başpınar N, Bucak MN, Akalın PP, Ataman MB, Ömür AD, Güngör Ş, Küçükgünay S, Özkalp B, Sarıözkan S. Influence of methionine and dithioerythritol on sperm motility, lipid peroxidation and antioxidant capacities during liquid storage of ram semen. Res Vet Sci 2010; 89: 426–431
  2. Bucak MN, Başpınar N, Tuncer PB, Sarıözkan S, Akalın PP, Çoyan K. Effects of curcumin and dithioerythritol on frozen-thawed bovine semen. Andrologia 2012; 44: 102–109
  3. Nyborg WL. Acoustic Streaming. Vol 2B. New York, NY, USA: Academic Press; 1965
  4. Suslick KS. Ultrasound: Its Chemical, Physical, and Biological Effects. New York, NY, USA: VCH Publishers; 1988
  5. Kuldiloke J. Effect of ultrasound, temperature and pressure treatments on enzyme activity and quality indicators of fruit and vegetable juices. PhD, Technische Universität, Berlin, Germany, 2002
  6. Burden DW. Guide to the disruption of biological samples. Random Primers 2012; 12: 1–25
  7. Halliday D, Resnick R, 1992. Fiziğin Temelleri (Translator: Cengiz Yalçın). 3rd ed. Ankara, Turkey: Arkadaş Press (in Turkish)
  8. Thompson LH, Doraiswamy LK. Sonochemistry: science and engineering. Ind Eng Chem Res 1999; 38: 1215–1249
  9. Mercan U. Toksikolojide serbest radikallerin önemi. YYU Vet Fak Derg 2004; 15: 91–96 (in Turkish)
  10. Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 1995; 4: 1819–1828
  11. Karabiga M, Kiriş İ, Yılmaz N, Altuntaş İ, Karahan N, Okutan H. Aprotinin’in deneysel aortik iskemi reperfüzyon modelinde böbrek hasarı üzerine etkisi. Turkish J Vasc Surg 2007; 165: 9–18
  12. Yajima D, Motani H, Hayakawa M, Sato Y, Sato K, Iwase H. The relationship between cell membrane damage and lipid peroxidation under the condition of hypoxia-reoxygenation: analysis of the mechanism using antioxidants and electron transport inhibitors. Cell Biochem Funct 2009; 27: 338–343
  13. Wong-Ekkabut J, Xu Z, Triampo W, Tang IM, Tieleman DP, Monticelli L. Effect of lipid peroxidation on the properties of lipid bilayers: A molecular dynamics study. Biophys J 2007; 12: 4225–4236
  14. Tateno H, Kimura Y, Yanagimachi R. Sonication per se is not as deleterious to sperm chromosomes as previously inferred. Biol Reprod 2000; 63: 341–346
  15. Yamamoto T, Yoneyama M, Imanishi M, Takeuchi M. Flow cytometric detection and analysis of tailless sperm caused by sonication or a chemical agent. Toxicol Sci 2000; 25: 41–48
  16. Baker SS, Cardullo RA, Thaler CD. Sonication of mouse sperm membran