Journals / Turkish Journal of Veterinary and Animal Sciences / 2019 / Cilt: 43 - Sayı: 6

Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue

Pages
834–845
Publication Date
2022-11-04
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Inconclusion, ADSCs were reported in detail here based on surface markers and differentiation potentials, growth curves, and populationdoubling times during the first, second, and third passages.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period.
📊
Key Findings & Data:Stem cells were characterized by immunostaining ofCD90, CD105, CD11b, and CD45 surface markers.
💡
Conclusion & Impact:Inconclusion, ADSCs were reported in detail here based on surface markers and differentiation potentials, growth curves, and populationdoubling times during the first, second, and third passages.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period. Adiposetissue-derived mesenchymal stem cells (ADSCs) are known to differentiate into many different organ and tissue cells. The purposeof this study was to characterize adipose tissue-derived mesenchymal stem cells in detail based on the expression status of certainsurface markers and differentiation stages for clinical and laboratory applications. The migrating phase and mitotic property of the cellsfrom adipose tissue were assessed under a microscope. Cell counting was performed during the first, second, and third passages. Thecells were differentiated into osteogenic, chondrogenic, and adipogenic lineages. Stem cells were characterized by immunostaining ofCD90, CD105, CD11b, and CD45 surface markers. ADSC growth kinetics were determined at the first, second, and third passages. Inconclusion, ADSCs were reported in detail here based on surface markers and differentiation potentials, growth curves, and populationdoubling times during the first, second, and third passages.

🔖

Cite This Article

ÇERÇİ, E., ERDOST, H. (2022). Phenotypic characterization and differentiation of mesenchymal stem cells originating from adipose tissue. Turkish Journal of Veterinary and Animal Sciences, Cilt: 43 - Sayı: 6, 834–845. https://ajindex.com/articles/614888

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Rodbell M. Metabolism of isolated fat cells. Journal of Biological Chemistry 1967; 242: 5751-5756
  2. Liu L, Cheung TH, Charville GW, Rando TA. Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting. Nature Protocols 2015; 10: 1612-1624
  3. Hayashi O, Katsube Y, Hirose M, Ohgushi H, Ito H. Comparison of osteogenic ability of rat mesenchymal stem cells from bone marrow, periosteum, and adipose tissue. Calcified Tissue International 2008; 82: 238-247
  4. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F et al. Minimal criteria for defining multipotent mesenchymal stromal cells. Cytotherapy 2006; 8: 315-317
  5. Miguélez-Rivera L, Pérez-Castrillo S, González-Fernández ML, Prieto-Fernández JG, López-González ME et al. Immunomodulation of mesenchymal stem cells in discogenic pain. North American Spine Society 2018, 2: 330-342
  6. Nancy P, Shilpita S, Sen M, Swathi S. Explant culture: a simple, reproducible, efficient and economic technique for isolation of mesenchymal stromal cells from human adipose tissue and lipoaspirate. Journal of Tissue Engineering and Regenerative Medicine 2012; 4: 524-531
  7. Braun J, Hack A, Weis-Klemm M, Conrad S, Treml S et al. Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells. American Journal of Veterinary Research 2010; 71: 1228- 1236
  8. Mizuno H, Tobita M, Uysal AC. Concise review: adipose-derived stem cells as a novel tool for future regenerative medicine. Stem Cells 2013; 30: 804-810
  9. Szöke K, Daňková J, Buzgo M, Amler E, Brinchmann JE et al. The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow. Process Biochemistry 2017; 59: 321-328
  10. Tajima S, Tobita M, Mizuno H. Bone regeneration with a combination of adipose-derived stem cells and platelet-rich plasma. Methods in Molecular Biology 2018; 97331: 372-387
  11. Tropel P, Noël D, Platet N, Legrand P, Benabid AL et al. Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Experimental Cell Research 2004; 295: 395-406
  12. Bonab MM, Alimoghaddam K, Talebian F, Ghaffari SH, Ghavamzadeh A et al. Aging of mesenchymal stem cell in vitro. BMC Molecular and Cell Biology 2006; 7: 14
  13. Kobolak J, Dinnyes A, Memic A, Khademhosseini A, Mobasheri A. Mesenchymal stem cells: identification, phenotypic characteriz