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Journals / Turkish Journal of Veterinary and Animal Sciences / 2019 / Cilt: 43 - Sayı: 4

In vitro effect of recombinant human gonadotropins on meiotic competence of dog oocytes

Mithat EVECEN
Kamber DEMİR
Kemal AK
Sema BİRLER
Ramazan ARICI
Serhat PABUCCUOĞLU
Hatem ATALLA
Nilhan ÇOŞKUN
Nur ERSOY
Selin YAĞCIOĞLU
Pages
469–473
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion,recombinant human gonadotropins could be applied for IVM of dog oocytes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The reproductive biology of the domestic dog is unique among mammalian species; because of this, in vitro maturation (IVM)rate is still very low compared with other domestic animals in spite of attempts at improvement.
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Key Findings & Data:A total of 845 cumulus–oocyte complexes were used in this study.
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Conclusion & Impact:In conclusion,recombinant human gonadotropins could be applied for IVM of dog oocytes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The reproductive biology of the domestic dog is unique among mammalian species; because of this, in vitro maturation (IVM)rate is still very low compared with other domestic animals in spite of attempts at improvement. The aim of this study was to consider theuse of recombinant human gonadotropins as a replacement for pituitary gonadotropins for IVM of dog oocytes. A total of 845 cumulus–oocyte complexes were used in this study. To determine the effects of human recombinant gonadotropins, maturation medium wassupplemented with two different concentrations (0.05 or 0.1 IU/mL) of pituitary (pFSH, pLH) and human recombinant (rhFSH andrhLH) gonadotropins. After the IVM period, the maturation rate of the oocytes was investigated under an epifluorescence microscope.Our findings showed no significant difference in maturation rate using either pituitary or human recombinant gonadotropin groups(P > 0.05). Applying 1.0 IU human recombinant gonadotropin caused the lowest maturation rate (34.57%; P < 0.05). In conclusion,recombinant human gonadotropins could be applied for IVM of dog oocytes. Moreover, 0.05 IU/mL rhFSH and rhLH can be successfullyused in place of biologically derived hormones.

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Cite This Article

EVECEN, M., DEMİR, K., AK, K., BİRLER, S., ARICI, R., PABUCCUOĞLU, S., ATALLA, H., ÇOŞKUN, N., ERSOY, N., YAĞCIOĞLU, S. (2022). In vitro effect of recombinant human gonadotropins on meiotic competence of dog oocytes. Turkish Journal of Veterinary and Animal Sciences, Cilt: 43 - Sayı: 4, 469–473. https://ajindex.com/articles/614835

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